| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,711,911 | T→C | 14.3% | S24G (AGC→GGC) | paaB ← | ring 1,2‑phenylacetyl‑CoA epoxidase regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,711,911 | 0 | T | C | 14.3% | 62.2 / 4.2 | 28 | S24G (AGC→GGC) | paaB | ring 1,2‑phenylacetyl‑CoA epoxidase regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base T (12/12); new base C (2/2); total (14/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
GGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGTTGCTTGCGCGCATAGGCCACTGCGG > NC_002947/3711768‑3712052 | ggCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGa < 8:88944/149‑1 (MQ=255) gTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGt > 7:122787/1‑149 (MQ=255) tcgGGAGAGGTGGCGGTGATCAGCGCCGAAGGCACCACCCTCAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGa < 3:7022/127‑1 (MQ=255) tcgGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGATCTCGCTGGACTACTAAAAATAAATAACAACATCGGCGTCGTGCACGCTGCCGa > 4:7022/1‑127 (MQ=255) ttaTCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAac < 5:264426/147‑1 (MQ=255) gcgcCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCACGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCg < 1:264318/149‑1 (MQ=255) agccTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGGACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGCCAt > 5:82036/4‑149 (MQ=255) tCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGTCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTa < 6:82880/127‑1 (MQ=255) tCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGTCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTa > 5:82880/1‑127 (MQ=255) tCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTc > 1:185741/1‑149 (MQ=255) acacCTTCGCTACGGCGGGTGTAGAGCTCGCGTGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTc < 4:288410/147‑1 (MQ=255) acCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCTGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGAtc < 6:285642/149‑1 (MQ=255) gggCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCATGCGGCGt > 7:291721/1‑148 (MQ=255) tcGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTGGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCt > 5:281751/1‑149 (MQ=255) gCCATGGCGGCATCGGCTGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACAGTTCGTAGAGGTTCAAGAGAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGttgc < 1:98441/148‑1 (MQ=255) aTGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAggcg > 1:172799/1‑127 (MQ=255) aTGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAggcg < 2:172799/127‑1 (MQ=255) ggcggcGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAggcg > 1:34519/1‑116 (MQ=255) ggcggcGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAggcg < 2:34519/116‑1 (MQ=255) ggcagcGTGCACGCCGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCtt > 3:292685/1‑124 (MQ=255) ggcagcGTGCACGCCGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCtt < 4:292685/124‑1 (MQ=255) ggcagcGTGCACGCCGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCtt > 3:297311/1‑124 (MQ=255) ggcagcGTGCACGCCGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTAtt < 4:297311/124‑1 (MQ=255) gTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGTTGCTTGCGCGCATAGGCCACTGCg > 4:88245/1‑149 (MQ=255) gTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGTTGCTTGCGCGCATAGGCCACTGCg < 8:122787/149‑1 (MQ=255) tGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGTTGCTTGCGCGCATAGGCCACTGCgg > 5:56846/1‑149 (MQ=255) cACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTc > 2:282282/1‑79 (MQ=255) cACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTc < 1:282282/79‑1 (MQ=255) | GGCGAACAGCGGGTCTTTCTCGTCGGGAGAGGAGGCGGTGATCAGCGCCGAAGGCACCACCCACAGACTCACACCTTCGCTACGGCGGGTGTAGAGCTCGCGGGCGTTCTCGATGGCCATGGCGGCATCGGCGGCGTGCACGCTGCCGACATGTTTGTGGTTAAGGCCGTGCTTGCTGCGCACGAACACTTCGTAGAGGGTCCAGACAGACATTTTCGATCTCCGCATCTATCGCCGCTCAGGCGGCGTTCTTGTTCTGTTGCTTGCGCGCATAGGCCACTGCGG > NC_002947/3711768‑3712052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |