Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,723,196 G→A 7.1% A151A (GCG→GCA)  PP_3288 → universal stress protein family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,723,1960GA 7.1% 161.5 / 2.3 56A151A (GCG→GCA) PP_3288universal stress protein family protein
Reads supporting (aligned to +/- strand):  ref base G (30/22);  new base A (2/2);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

ACACTTGCGTGAGCAACCGTTTGCCATGCTGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTGTACGCCTACGATTTGGGTT  >  NC_002947/3723049‑3723344
                                                                                                                                                   |                                                                                                                                                    
acacTTGCGTGAGCAACCGTTTGCCATGCTGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGt                                                                                                                                                     >  4:28221/1‑149 (MQ=255)
              aaCCGTTTGCCATGCTGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCATTGCCACGCAgg                                                                                                                                          <  4:308682/146‑1 (MQ=255)
              aaCCGTTTGCCATGCTGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCATTGCCACGCAgg                                                                                                                                          <  4:313661/146‑1 (MQ=255)
              aaCCGTTTGCCATGCTGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCATTGCCACGCAgg                                                                                                                                          >  3:313661/1‑146 (MQ=255)
              aaCCGTTTGCCATGCTGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCATTGCCACGCAgg                                                                                                                                          >  3:308682/1‑146 (MQ=255)
              aaCCGTTTGCCATGCTGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCAAGCCATGCGTTGCCACGCAGGGtt                                                                                                                                       >  3:165546/1‑149 (MQ=255)
                   tttGCCATGCTGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTggc                                                                                                                                  >  6:68230/1‑149 (MQ=255)
                             tGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTGCTGGAGGCGGTGGa                                                                                                                         >  6:224338/1‑148 (MQ=255)
                               aTCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCt                                                                                                                      >  6:189474/1‑149 (MQ=255)
                                tCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCt                                                                                                                      <  4:208638/148‑1 (MQ=255)
                                 cAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGt                                                                                                                    >  8:88817/1‑149 (MQ=255)
                                  aaaGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTc                                                                                                                   <  5:189474/149‑1 (MQ=255)
                                    agagCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTc                                                                                                                   >  2:160631/1‑147 (MQ=255)
                                        cTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGcc                                                                                                             >  7:114751/1‑149 (MQ=255)
                                         tCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCg                                                                                                            >  4:177740/1‑149 (MQ=255)
                                          cGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGtt                                                                                                                                       >  2:42020/1‑121 (MQ=255)
                                          cGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGtt                                                                                                                                       <  1:42020/121‑1 (MQ=255)
                                           gAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCg                                                                                                            >  7:270512/1‑147 (MQ=255)
                                              cATGAGACTTTGTGGATGAGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGAGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCgtagga                                                                                                       >  6:189056/1‑149 (MQ=255)
                                               aTGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGAt                                                                                                      <  8:270512/149‑1 (MQ=255)
                                                   gCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGt                                                                                                  <  3:177740/149‑1 (MQ=255)
                                                        ggtggATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTg                                                                                               >  7:255572/1‑147 (MQ=255)
                                                             aTGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCtt                                                                                        <  1:160631/149‑1 (MQ=255)
                                                                    cAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGAc                                                                                 <  4:295845/149‑1 (MQ=255)
                                                                         ttCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGa                                                                             >  7:69088/1‑148 (MQ=255)
                                                                         ttCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGa                                                                             <  7:14505/148‑1 (MQ=255)
                                                                           cACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCCGAGGATCAGTTTGAAGGCTTCAACGACCAGatc                                                                           <  1:206042/148‑1 (MQ=255)
                                                                                     gATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAgc                                                                >  6:32825/1‑149 (MQ=255)
                                                                                      atatCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAgcg                                                               <  5:68230/149‑1 (MQ=255)
                                                                                          ccAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTg                                                            >  1:142404/1‑148 (MQ=255)
                                                                                          ccAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTg                                                            >  4:87010/1‑148 (MQ=255)
                                                                                            aGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATc                                                                                                     <  1:95138/105‑1 (MQ=255)
                                                                                            aGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATc                                                                                                     >  2:95138/1‑105 (MQ=255)
                                                                                                 ctgcGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGa                                                     <  8:142105/148‑1 (MQ=255)
                                                                                                 ctgcGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGAGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGa                                                     <  5:224338/148‑1 (MQ=255)
                                                                                                   gcgcGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACAt                                                  >  8:8045/1‑149 (MQ=255)
                                                                                                     gcgAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTg                                                <  7:88817/149‑1 (MQ=255)
                                                                                                         aaCGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCAGGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGt                                            <  5:189056/149‑1 (MQ=255)
                                                                                                           cgcgAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTg                                           >  4:190664/1‑148 (MQ=255)
                                                                                                                    ccTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCa                                              <  5:126232/136‑1 (MQ=255)
                                                                                                                    ccTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCa                                              >  6:126232/1‑136 (MQ=255)
                                                                                                                    ccTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGcc                                    >  4:55319/1‑146 (MQ=255)
                                                                                                                    ccTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGcc                                    >  3:224930/1‑146 (MQ=255)
                                                                                                                     cTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGcc                                    >  4:19638/1‑145 (MQ=255)
                                                                                                                             tGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAg                           <  7:249615/146‑1 (MQ=255)
                                                                                                                             tGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAg                           >  8:249615/1‑146 (MQ=255)
                                                                                                                               gTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCt                      <  8:69088/149‑1 (MQ=255)
                                                                                                                                tACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTg                     <  7:8045/149‑1 (MQ=255)
                                                                                                                                 acacAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGAt                               >  3:180561/1‑138 (MQ=255)
                                                                                                                                 acacAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGAt                               <  4:180561/138‑1 (MQ=255)
                                                                                                                                 acacAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTg                     >  3:117386/1‑148 (MQ=255)
                                                                                                                                   acaAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTGTAc                  >  6:97372/1‑149 (MQ=255)
                                                                                                                                       ggCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTGTACGcc               >  2:86225/1‑148 (MQ=255)
                                                                                                                                        gccagccaTGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTGTACGCCTa             >  3:95200/1‑149 (MQ=255)
                                                                                                                                               aTGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTGTACGCCTACGATTTg      <  3:19638/149‑1 (MQ=255)
                                                                                                                                                  cGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTGTACGCCTACGATTTggg    <  3:28221/148‑1 (MQ=255)
                                                                                                                                                   gTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTGTACGCCTACGATTTGGGtt  >  6:55914/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACACTTGCGTGAGCAACCGTTTGCCATGCTGATCAAAGAGCTCGAGCATGAGCCTTGGTGGATGCGCGCAATGTTCACTTCGCTGGATATCCAGTTGCTGCGCGAAACGCGAATACCCTTGCACCTGGTACACAAGGCCAGCCATGCGTTGCCACGCAGGGTTCTGGCGGCGGTGGACCTGTCCAGGCCGGAGGATCAGTTTGAAGGCTTCAACGACCAGATCATCAGCGAAGCGCTGAAGCTGACATTGCAGTGCAATGCCCAGATCGAGTTGCTGTACGCCTACGATTTGGGTT  >  NC_002947/3723049‑3723344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑