Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,757,027 T→G 10.4% M30L (ATG→CTG)  PP_3320 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,757,0270TG10.4% 126.3 / 6.0 48M30L (ATG→CTG) PP_3320hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/23);  new base G (3/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

TAGGCGTCAGCGTTGGATGCCAGCACGATATGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATGATTTGCAACCTGC  >  NC_002947/3756883‑3757173
                                                                                                                                                |                                                                                                                                                  
taGGCGTCAGCGTTGGATGCCAGCACGATATGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAg                                                                                                                                                 <  5:311267/148‑1 (MQ=255)
 aGGCGTCAGCGTTGGATGCCAGCACGATATGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGa                                                                                                                                               >  1:266159/1‑149 (MQ=255)
           gTTGGATGCCAGCACGATATGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGAt                                                                                                                                     <  2:266159/149‑1 (MQ=255)
              ggATGCCAGCACGATATGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGt                                                                                                                                   <  4:160147/148‑1 (MQ=255)
               gATGCCAGCACGATATGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCa                                                                                                                                 >  8:244214/1‑149 (MQ=255)
                 tGCCAGCACGATATGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCAtg                                                                                                                               <  7:244214/149‑1 (MQ=255)
                     aGCACGATATGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCAtgctgc                                                                                                                           >  4:265140/1‑149 (MQ=255)
                           atatGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCAGCATGACATCGGCGAAGTCCTGATGCTggcct                                                                                                                     <  5:88884/149‑4 (MQ=38)
                                cTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGc                                                                                                                >  1:218161/1‑149 (MQ=255)
                                   aCATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTAGCCGATGTCATGCTGCTGGGTGATGGCggg                                                                                                             >  8:201622/1‑149 (MQ=255)
                                     aTTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGAGGGGa                                                                                                           >  3:164822/1‑149 (MQ=255)
                                           ctctTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGcc                                                                                                     >  5:294947/1‑149 (MQ=255)
                                              ttctCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGccc                                                                                                    >  5:315040/1‑147 (MQ=255)
                                                    acaTCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGt                                                                                            >  4:286828/1‑149 (MQ=255)
                                                    acaTCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGt                                                                                            <  4:154605/149‑1 (MQ=255)
                                                         ccGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAgggg                                                                                              <  5:74961/142‑1 (MQ=255)
                                                         ccGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAgggg                                                                                              >  6:74961/1‑142 (MQ=255)
                                                         ccGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAg                                                                                       >  2:251172/1‑149 (MQ=255)
                                                          cGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAgg                                                                                      <  3:265140/149‑1 (MQ=255)
                                                                tCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTAtgt                                                                                >  2:195703/1‑149 (MQ=255)
                                                                  aTAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTAtgtg                                                                               <  1:251172/148‑1 (MQ=255)
                                                                  aTAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTgg                                                                              <  1:54293/149‑1 (MQ=255)
                                                                        tAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGc                                                                        <  1:195703/149‑1 (MQ=255)
                                                                            aaGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACAt                                                                    >  8:50891/1‑149 (MQ=255)
                                                                                gcCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGt                                                                <  4:164822/149‑1 (MQ=255)
                                                                                  ccAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGc                                                                        <  4:245771/139‑1 (MQ=255)
                                                                                  ccAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGc                                                                        >  3:245771/1‑139 (MQ=255)
                                                                                                 aCCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTc                                               >  5:283574/1‑149 (MQ=255)
                                                                                                 aCCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTc                                               >  8:197372/1‑149 (MQ=255)
                                                                                                  ccAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTcc                                              >  6:179508/1‑149 (MQ=255)
                                                                                                   cAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTccc                                             <  6:294947/149‑1 (MQ=255)
                                                                                                       aaTGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCt                                         <  2:61352/149‑1 (MQ=255)
                                                                                                           tACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGc                                     <  7:50891/149‑1 (MQ=255)
                                                                                                                aaGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCt                                >  2:6335/1‑149 (MQ=255)
                                                                                                                  gcagcGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTc                               >  7:35328/1‑148 (MQ=255)
                                                                                                                     gcgcCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAgg                           <  8:35328/149‑1 (MQ=255)
                                                                                                                     gcgcCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAgg                           <  6:315040/149‑1 (MQ=255)
                                                                                                                            ttCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAg                      <  7:197372/147‑1 (MQ=255)
                                                                                                                                      cAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATGATTTg          <  3:286828/149‑1 (MQ=255)
                                                                                                                                       aGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATGATTTGc         <  5:179508/149‑1 (MQ=255)
                                                                                                                                         gCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATg               <  2:165040/141‑1 (MQ=255)
                                                                                                                                         gCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATg               >  1:165040/1‑141 (MQ=255)
                                                                                                                                          ccATCATCAGGACTTCGCCGATGTCATGCTTCTGGGTGATGGCGGGGATCATCCCCAGGGGGTTCCAGTGTATGTTGCGAAGCACATCGGTCAATGCGAATCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATGATTTGCAAc      <  7:201622/149‑1 (MQ=255)
                                                                                                                                          ccAGCAGCATGACATCGGCGAAGTCCTGATGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATg               <  3:170680/140‑1 (MQ=38)
                                                                                                                                          ccAGCAGCATGACATCGGCGAAGTCCTGATGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATg               >  4:170680/1‑140 (MQ=38)
                                                                                                                                          ccAGCAGCATGACATCGGCGAAGTCCTGATGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATGATTTGCAAc      >  7:63811/1‑149 (MQ=39)
                                                                                                                                          ccAGCAGCATGACATCGGCGAAGTCCTGATGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATGATTTGCAAc      >  7:94899/1‑149 (MQ=39)
                                                                                                                                              cATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATGATTTGCAACCTGc  <  6:283574/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TAGGCGTCAGCGTTGGATGCCAGCACGATATGCTGACATTTCCCTCTTCTCCACATCCCGTTTGTCATAACGTAACAAGCGCCCAGAGTTGCTGAGCACCAGCAATGTACTGAAGCAGCGCCTTTTCGTTCATCCAGGCCAGCATCAGGACTTCGCCGATGTCATGCTGCTGGGTGATGGCGGGGATCAGCCCCAGGGGGTTCCAGGGTATGTGGCGAAGCACATCGGTCAAGGCGAAGCGGCTTCCCGCCTGTGCCTGCTCAAGGGTAAGGAACATGATTTGCAACCTGC  >  NC_002947/3756883‑3757173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑