Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,761,701 C→T 11.7% A350V (GCC→GTC) ‡ PP_3325 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,761,7010CT11.7% 131.5 / 7.6 52A350V (GCC→GTC) ‡PP_3325ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base C (25/20);  new base T (3/3);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

GAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACG  >  NC_002947/3761564‑3761847
                                                                                                                                           |                                                                                                                                                  
gAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGcc                                                                                                                                                   <  8:302894/139‑1 (MQ=255)
gAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGcc                                                                                                                                                   >  7:302894/1‑139 (MQ=255)
gAAGCTGCGCCTGGCCAACCACTACACCCAGCGCACGCTCAAGGGCCACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACAATCACGCGCTTCTATCGCGAACCCTCCTACGAA‑‑TGGAACGACAACATCACCCCGGCCGAGCTCCACg                                                                                                                                         >  3:1605/1‑149 (MQ=255)
    cTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACggg                                                                                                                                       >  5:81365/1‑147 (MQ=255)
      gcgcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGt                                                                                                                                   >  2:72736/1‑149 (MQ=255)
        gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                 >  7:290477/1‑149 (MQ=255)
        gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                 >  3:30617/1‑149 (MQ=255)
              ccAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCggcctg                                                                                                                           <  1:121047/149‑6 (MQ=255)
               cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTgg                                                                                                                          >  2:217295/1‑149 (MQ=255)
               cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGACCTgg                                                                                                                          >  7:270663/1‑149 (MQ=255)
                 aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCCCCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTg                                                                                                                           <  3:4603/146‑1 (MQ=255)
                 aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGCGCTGCACGGGCAGTTCGAGCTg                                                                                                                           >  4:4603/1‑146 (MQ=255)
                   cACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtgg                                                                                                                      >  6:105419/1‑149 (MQ=255)
                    aCTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggc                                                                                                                     >  8:204138/1‑149 (MQ=255)
                        cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc                                                                                                                 <  6:81365/149‑1 (MQ=255)
                        cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc                                                                                                                 <  3:1106/149‑1 (MQ=255)
                         acCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgca                                                                                                                <  2:66060/149‑1 (MQ=255)
                           ccAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcag                                                                                                               >  2:99361/1‑148 (MQ=255)
                            cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga                                                                                                                      >  2:231089/1‑139 (MQ=38)
                            cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga                                                                                                                      <  1:231089/140‑2 (MQ=38)
                             aGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCAt                                                                                                            <  1:99361/149‑1 (MQ=255)
                              gggCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATc                                                                                                           <  4:117218/149‑1 (MQ=255)
                                    gCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGAccc                                                                                                     <  8:290477/149‑1 (MQ=255)
                                         aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg                                                                                                <  4:231460/149‑1 (MQ=255)
                                         aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg                                                                                                <  1:72736/149‑1 (MQ=255)
                                         aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtgggg                                                                                                <  7:204138/149‑3 (MQ=255)
                                          gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGc                                                                                                                                      >  3:3912/1‑110 (MQ=255)
                                          gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGc                                                                                                                                      <  4:3912/110‑1 (MQ=255)
                                                   cTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAAGGTGGACCGACAACATCACCCAGGCCGAGCTGCACGGGATGTTCGCGGTGGGTGGCTGGCAGCAACCGCCCCTGGTGGGGGTg                                                                                          >  1:258054/1‑147 (MQ=255)
                                                                      gTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAg                                                                   >  5:38636/1‑149 (MQ=255)
                                                                              caccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAg                                                            >  1:216265/1‑148 (MQ=255)
                                                                                ccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAc                                                         <  8:270663/149‑1 (MQ=255)
                                                                                      cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc                                                        >  1:133694/1‑144 (MQ=255)
                                                                                      cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc                                                        <  2:133694/144‑1 (MQ=255)
                                                                                         gcTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAgc                                                <  5:105419/149‑1 (MQ=255)
                                                                                             cTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCa                                            >  7:47553/1‑149 (MQ=255)
                                                                                                tCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGt                                         >  3:202847/1‑149 (MQ=255)
                                                                                                  gcgAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTc                                        <  4:74946/148‑1 (MQ=255)
                                                                                                        gcgACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCg                                 >  8:163039/1‑149 (MQ=255)
                                                                                                          gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                  <  2:317189/66‑1 (MQ=255)
                                                                                                          gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                  >  1:317189/1‑66 (MQ=255)
                                                                                                          gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa                               >  5:20336/1‑149 (MQ=255)
                                                                                                          gACTCCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa                               >  2:89168/1‑149 (MQ=255)
                                                                                                             ttCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGGGCTGGGTGGCTGGCAGCATCAGCGCCTGGTGGGGCTGGAGTACGAACACTACCGCGACAGCCAGAAGTACCCGCACAGCGCCCCGTCCGCAGCGTATgg                            >  8:211661/1‑149 (MQ=255)
                                                                                                               cGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGc                          >  8:59350/1‑149 (MQ=255)
                                                                                                                  a‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTgg                       <  1:89168/149‑1 (MQ=255)
                                                                                                                         aCGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATc                  <  4:202847/149‑1 (MQ=255)
                                                                                                                               acaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAAc            <  1:217295/149‑1 (MQ=255)
                                                                                                                                  tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg                                                                                         >  4:107244/1‑69 (MQ=255)
                                                                                                                                  tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg                                                                                         <  3:107244/69‑1 (MQ=255)
                                                                                                                                   cACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCgg        >  8:14469/1‑149 (MQ=38)
                                                                                                                                      ccAGGGCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGta      >  7:291050/1‑148 (MQ=255)
                                                                                                                                        aGGCCGCGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATAc   <  7:211661/149‑1 (MQ=255)
                                                                                                                                         ggCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACg  >  1:105531/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                  
GAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACG  >  NC_002947/3761564‑3761847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑