Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,761,705	G→A	11.3%	E351E (GAG→GAA)	PP_3325 →	ferric siderophore receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,761,705	0	G	A	11.3%	137.9 / 6.7	53	E351E (GAG→GAA)	PP_3325	ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (26/21);  new base A (3/3);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-01

GAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAG  >  NC_002947/3761564‑3761852                                                                                                                                                |                                                                                                                                                    gAAGCTGCGCCTGGCCAACCACTACACCCAGCGCACGCTCAAGGGCCACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACAATCACGCGCTTCTATCGCGAACCCTCCTACGAA‑‑TGGAACGACAACATCACCCCGGCCGAGCTCCACg                                                                                                                                              >  3:1605/1‑149 (MQ=255)     cTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACggg                                                                                                                                            >  5:81365/1‑147 (MQ=255)       gcgcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGt                                                                                                                                        >  2:72736/1‑149 (MQ=255)         gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                      >  7:290477/1‑149 (MQ=255)         gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                      >  3:30617/1‑149 (MQ=255)               ccAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCggcctg                                                                                                                                <  1:121047/149‑6 (MQ=255)                cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTgg                                                                                                                               >  2:217295/1‑149 (MQ=255)                cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGACCTgg                                                                                                                               >  7:270663/1‑149 (MQ=255)                  aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCCCCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTg                                                                                                                                <  3:4603/146‑1 (MQ=255)                  aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGCGCTGCACGGGCAGTTCGAGCTg                                                                                                                                >  4:4603/1‑146 (MQ=255)                    cACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtgg                                                                                                                           >  6:105419/1‑149 (MQ=255)                     aCTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggc                                                                                                                          >  8:204138/1‑149 (MQ=255)                         cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc                                                                                                                      <  6:81365/149‑1 (MQ=255)                         cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc                                                                                                                      <  3:1106/149‑1 (MQ=255)                          acCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgca                                                                                                                     <  2:66060/149‑1 (MQ=255)                            ccAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcag                                                                                                                    >  2:99361/1‑148 (MQ=255)                             cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga                                                                                                                           >  2:231089/1‑139 (MQ=38)                             cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga                                                                                                                           <  1:231089/140‑2 (MQ=38)                              aGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCAt                                                                                                                 <  1:99361/149‑1 (MQ=255)                               gggCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATc                                                                                                                <  4:117218/149‑1 (MQ=255)                                     gCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGAccc                                                                                                          <  8:290477/149‑1 (MQ=255)                                          aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg                                                                                                     <  1:72736/149‑1 (MQ=255)                                          aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg                                                                                                     <  4:231460/149‑1 (MQ=255)                                          aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtgggg                                                                                                     <  7:204138/149‑3 (MQ=255)                                           gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGc                                                                                                                                           <  4:3912/110‑1 (MQ=255)                                           gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGc                                                                                                                                           >  3:3912/1‑110 (MQ=255)                                                    cTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAAGGTGGACCGACAACATCACCCAGGCCGAGCTGCACGGGATGTTCGCGGTGGGTGGCTGGCAGCAACCGCCCCTGGTGGGGGTg                                                                                               >  1:258054/1‑147 (MQ=255)                                                                       gTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAg                                                                        >  5:38636/1‑149 (MQ=255)                                                                               caccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAg                                                                 >  1:216265/1‑148 (MQ=255)                                                                                 ccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAc                                                              <  8:270663/149‑1 (MQ=255)                                                                                       cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc                                                             <  2:133694/144‑1 (MQ=255)                                                                                       cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc                                                             >  1:133694/1‑144 (MQ=255)                                                                                          gcTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAgc                                                     <  5:105419/149‑1 (MQ=255)                                                                                              cTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCa                                                 >  7:47553/1‑149 (MQ=255)                                                                                                 tCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGt                                              >  3:202847/1‑149 (MQ=255)                                                                                                   gcgAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTc                                             <  4:74946/148‑1 (MQ=255)                                                                                                         gcgACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCg                                      >  8:163039/1‑149 (MQ=255)                                                                                                           gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                       <  2:317189/66‑1 (MQ=255)                                                                                                           gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                       >  1:317189/1‑66 (MQ=255)                                                                                                           gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa                                    >  5:20336/1‑149 (MQ=255)                                                                                                           gACTCCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa                                    >  2:89168/1‑149 (MQ=255)                                                                                                              ttCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGGGCTGGGTGGCTGGCAGCATCAGCGCCTGGTGGGGCTGGAGTACGAACACTACCGCGACAGCCAGAAGTACCCGCACAGCGCCCCGTCCGCAGCGTATgg                                 >  8:211661/1‑149 (MQ=255)                                                                                                                cGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGc                               >  8:59350/1‑149 (MQ=255)                                                                                                                   a‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTgg                            <  1:89168/149‑1 (MQ=255)                                                                                                                          aCGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATc                       <  4:202847/149‑1 (MQ=255)                                                                                                                                acaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAAc                 <  1:217295/149‑1 (MQ=255)                                                                                                                                   tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg                                                                                              >  4:107244/1‑69 (MQ=255)                                                                                                                                   tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg                                                                                              <  3:107244/69‑1 (MQ=255)                                                                                                                                    cACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCgg             >  8:14469/1‑149 (MQ=38)                                                                                                                                       ccAGGGCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGta           >  7:291050/1‑148 (MQ=255)                                                                                                                                         aGGCCGCGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATAc        <  7:211661/149‑1 (MQ=255)                                                                                                                                          ggCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACg       >  1:105531/1‑149 (MQ=255)                                                                                                                                               aGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAg  <  4:30617/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                    GAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAG  >  NC_002947/3761564‑3761852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑