Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,761,710	A→C	10.7%	H353P (CAC→CCC)	PP_3325 →	ferric siderophore receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,761,710	0	A	C	10.7%	144.8 / 9.7	56	H353P (CAC→CCC)	PP_3325	ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base C (3/3);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

GAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAG  >  NC_002947/3761564‑3761858                                                                                                                                                     |                                                                                                                                                     gAAGCTGCGCCTGGCCAACCACTACACCCAGCGCACGCTCAAGGGCCACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACAATCACGCGCTTCTATCGCGAACCCTCCTACGAA‑‑TGGAACGACAACATCACCCCGGCCGAGCTCCACg                                                                                                                                                    >  3:1605/1‑149 (MQ=255)     cTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACggg                                                                                                                                                  >  5:81365/1‑147 (MQ=255)       gcgcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGt                                                                                                                                              >  2:72736/1‑149 (MQ=255)         gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                            >  7:290477/1‑149 (MQ=255)         gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                            >  3:30617/1‑149 (MQ=255)               ccAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCggcctg                                                                                                                                      <  1:121047/149‑6 (MQ=255)                cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTgg                                                                                                                                     >  2:217295/1‑149 (MQ=255)                cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGACCTgg                                                                                                                                     >  7:270663/1‑149 (MQ=255)                  aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCCCCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTg                                                                                                                                      <  3:4603/146‑1 (MQ=255)                  aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGCGCTGCACGGGCAGTTCGAGCTg                                                                                                                                      >  4:4603/1‑146 (MQ=255)                    cACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtgg                                                                                                                                 >  6:105419/1‑149 (MQ=255)                     aCTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggc                                                                                                                                >  8:204138/1‑149 (MQ=255)                         cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc                                                                                                                            <  3:1106/149‑1 (MQ=255)                         cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc                                                                                                                            <  6:81365/149‑1 (MQ=255)                          acCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgca                                                                                                                           <  2:66060/149‑1 (MQ=255)                            ccAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcag                                                                                                                          >  2:99361/1‑148 (MQ=255)                             cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga                                                                                                                                 >  2:231089/1‑139 (MQ=38)                             cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga                                                                                                                                 <  1:231089/140‑2 (MQ=38)                              aGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCAt                                                                                                                       <  1:99361/149‑1 (MQ=255)                               gggCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATc                                                                                                                      <  4:117218/149‑1 (MQ=255)                                     gCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGAccc                                                                                                                <  8:290477/149‑1 (MQ=255)                                          aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg                                                                                                           <  4:231460/149‑1 (MQ=255)                                          aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg                                                                                                           <  1:72736/149‑1 (MQ=255)                                          aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtgggg                                                                                                           <  7:204138/149‑3 (MQ=255)                                           gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGc                                                                                                                                                 >  3:3912/1‑110 (MQ=255)                                           gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGc                                                                                                                                                 <  4:3912/110‑1 (MQ=255)                                                    cTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAAGGTGGACCGACAACATCACCCAGGCCGAGCTGCACGGGATGTTCGCGGTGGGTGGCTGGCAGCAACCGCCCCTGGTGGGGGTg                                                                                                     >  1:258054/1‑147 (MQ=255)                                                                       gTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAg                                                                              >  5:38636/1‑149 (MQ=255)                                                                               caccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAg                                                                       >  1:216265/1‑148 (MQ=255)                                                                                 ccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAc                                                                    <  8:270663/149‑1 (MQ=255)                                                                                       cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc                                                                   >  1:133694/1‑144 (MQ=255)                                                                                       cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc                                                                   <  2:133694/144‑1 (MQ=255)                                                                                          gcTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAgc                                                           <  5:105419/149‑1 (MQ=255)                                                                                              cTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCa                                                       >  7:47553/1‑149 (MQ=255)                                                                                                 tCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGt                                                    >  3:202847/1‑149 (MQ=255)                                                                                                   gcgAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTc                                                   <  4:74946/148‑1 (MQ=255)                                                                                                         gcgACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCg                                            >  8:163039/1‑149 (MQ=255)                                                                                                           gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                             <  2:317189/66‑1 (MQ=255)                                                                                                           gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                             >  1:317189/1‑66 (MQ=255)                                                                                                           gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa                                          >  5:20336/1‑149 (MQ=255)                                                                                                           gACTCCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa                                          >  2:89168/1‑149 (MQ=255)                                                                                                              ttCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGGGCTGGGTGGCTGGCAGCATCAGCGCCTGGTGGGGCTGGAGTACGAACACTACCGCGACAGCCAGAAGTACCCGCACAGCGCCCCGTCCGCAGCGTATgg                                       >  8:211661/1‑149 (MQ=255)                                                                                                                cGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGc                                     >  8:59350/1‑149 (MQ=255)                                                                                                                   a‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTgg                                  <  1:89168/149‑1 (MQ=255)                                                                                                                          aCGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATc                             <  4:202847/149‑1 (MQ=255)                                                                                                                                acaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAAc                       <  1:217295/149‑1 (MQ=255)                                                                                                                                   tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg                                                                                                    >  4:107244/1‑69 (MQ=255)                                                                                                                                   tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg                                                                                                    <  3:107244/69‑1 (MQ=255)                                                                                                                                    cACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCgg                   >  8:14469/1‑149 (MQ=38)                                                                                                                                       ccAGGGCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGta                 >  7:291050/1‑148 (MQ=255)                                                                                                                                         aGGCCGCGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATAc              <  7:211661/149‑1 (MQ=255)                                                                                                                                          ggCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACg             >  1:105531/1‑149 (MQ=255)                                                                                                                                               aGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAg        <  4:30617/149‑1 (MQ=255)                                                                                                                                                 cTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACTCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGc       <  8:47553/148‑1 (MQ=255)                                                                                                                                                    cACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCg     <  7:59350/147‑1 (MQ=255)                                                                                                                                                    cACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGa    <  2:105531/148‑1 (MQ=255)                                                                                                                                                     aCGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAag  <  8:291050/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GAAGCTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAG  >  NC_002947/3761564‑3761858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑