Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 326,068 T→G 7.7% A555A (GCA→GCC)  PP_0269 ← glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947326,0680TG 7.7% 219.8 / 7.9 75A555A (GCA→GCC) PP_0269glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base G (4/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGG  >  NC_002947/325927‑326214
                                                                                                                                             |                                                                                                                                                  
cTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCgggg                                                                                                                                             <  7:98110/149‑1 (MQ=255)
cTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCgggg                                                                                                                                             <  7:272959/149‑1 (MQ=255)
        cTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTTATCTGATGTATCGAACAGCCCCGGCCTATGCCGTGGCTGTTTCt                                                                                                                                     >  6:178050/1‑149 (MQ=255)
        cTCGTTCATGCGTACCAACAACGCAGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCCTAATCGTCGAGTACCCGATGAGCATCTTCAGATTCTGATCTGATGTATCGAACACCCCCGGCCGATGAGGGGGAAGTTTCt                                                                                                                                     >  8:129165/1‑149 (MQ=255)
         tCGTTCTTGCGTACCTACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCTTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtt                                                                                                                                    <  1:2109/149‑1 (MQ=255)
           gTTCTTGCGTACCAACAACGCCGTGCATCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtttt                                                                                                                                  <  3:108481/149‑1 (MQ=255)
           gTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtt                                                                                                                                    <  6:255965/147‑1 (MQ=255)
            ttCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTc                                                                                                                                 <  4:199152/149‑1 (MQ=255)
            ttCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTc                                                                                                                                 >  2:304434/1‑149 (MQ=255)
            ttCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTc                                                                                                                                 >  8:37902/1‑149 (MQ=255)
                 gCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGc                                                                                                                              <  7:9443/147‑1 (MQ=255)
                  cGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGaa                                                                                                                           >  4:131420/1‑149 (MQ=255)
                       caacaaCGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACACGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCGGTTTCTTTTCGGCGAACGGt                                                                                                                       >  5:182769/1‑148 (MQ=255)
                        aacaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGGATCTTCTGATTCTGATCTGATGTATCGAACATCCCCGGCCTATGCCGGGGCTGTTTGTTTTCGGCGAACGGt                                                                                                                       >  6:164730/1‑147 (MQ=255)
                         acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAc                                                                                                                    >  1:69580/1‑149 (MQ=255)
                          caacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACt                                                                                                                   >  8:132300/1‑149 (MQ=255)
                                  tGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGc                                                                                                           <  1:59588/149‑1 (MQ=255)
                                   gcagcaGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGcc                                                                                                          >  7:282991/1‑149 (MQ=255)
                                    cagcagAACGGATACAAAGACGACGGCAACGAAGTCCTCGTATTCGTCGAGTACCCGATCTGCATCTTCTGATTCTTATCTGATGTATCTAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGcc                                                                                                          <  7:107993/148‑1 (MQ=255)
                                     agcagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGtt                                                                                                                <  2:64694/141‑1 (MQ=255)
                                     agcagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGtt                                                                                                                >  1:64694/1‑141 (MQ=255)
                                     agcagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCgg                                                                                                        <  7:132300/149‑1 (MQ=255)
                                       cagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtt                                                                                                                                    <  4:230859/119‑1 (MQ=255)
                                       cagAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATAGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtt                                                                                                                                    >  3:230859/1‑119 (MQ=255)
                                           aCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTgg                                                                                                  <  2:295660/149‑1 (MQ=255)
                                           aCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTgg                                                                                                  <  2:300475/149‑1 (MQ=255)
                                                  cAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACg                                                                                           >  3:14134/1‑149 (MQ=255)
                                                       acgacgGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGtctc                                                                                      <  4:14134/149‑1 (MQ=255)
                                                                aCGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCaaa                                                                             <  3:131420/149‑1 (MQ=255)
                                                                 cGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAg                                                                            <  6:76735/149‑1 (MQ=255)
                                                                 cGAAGTCAGCGTATTCTTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAg                                                                            <  5:178050/149‑1 (MQ=255)
                                                                   aaGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCt                                                                          <  5:44170/149‑1 (MQ=255)
                                                                     gTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGt                                                                        <  1:304434/149‑1 (MQ=255)
                                                                     gTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGt                                                                        <  7:37902/149‑1 (MQ=255)
                                                                        cgcgTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGc                                                                     >  7:172991/1‑149 (MQ=255)
                                                                         gcgTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTc                                                                       >  2:250827/1‑146 (MQ=255)
                                                                         gcgTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTc                                                                       <  1:250827/146‑1 (MQ=255)
                                                                             aTTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTAcgc                                                                >  1:65195/1‑149 (MQ=255)
                                                                                    gAGTACCCGATCAGCATCTTCTGATTCTGATCTGTTTTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATc                                                          <  6:182769/148‑1 (MQ=255)
                                                                                        aCCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGcc                                                      <  8:282991/148‑1 (MQ=255)
                                                                                          ccGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGt                                                                                                                 <  6:113816/87‑1 (MQ=255)
                                                                                          ccGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGt                                                                                                                 >  5:113816/1‑87 (MQ=255)
                                                                                          ccGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCcaca                                                   >  3:168885/1‑149 (MQ=255)
                                                                                               ccgcctcttctGATTGTGCTCTGCTGTATCGACCAGCGCCCGCCTCTGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCcgc                                              <  8:172991/144‑1 (MQ=255)
                                                                                               cAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCcgc                                              >  6:323561/1‑149 (MQ=255)
                                                                                                 gCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCcgcg                                             >  2:96588/1‑148 (MQ=255)
                                                                                                  cATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCgcgc                                            >  2:244315/1‑148 (MQ=255)
                                                                                                  cATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCCGTCGCTACGCGCCATCCGCCACATCCgcgc                                            <  5:230718/148‑1 (MQ=255)
                                                                                                  cATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCCGTCGCTACGCGCCATCCGCCACATCCgcgc                                            >  6:230718/1‑148 (MQ=255)
                                                                                                      ttctgattctgaTCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCCAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATaa                                        >  6:301447/1‑148 (MQ=255)
                                                                                                       tctgattctgatCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATaaa                                       >  4:183316/1‑148 (MQ=255)
                                                                                                        ctgattctgatCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTACTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAAc                                     >  5:7845/1‑149 (MQ=255)
                                                                                                        ctgattcggatCTGCTGTATCGAACATCCCCTGCCTAGGCCGGGGATGTTTCTTTTCTGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTTGCTACGCGGCATCCGCCACATCCGCGCATaaat                                      <  7:129165/148‑2 (MQ=255)
                                                                                                         tgattctgatCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACt                                    <  1:244315/149‑1 (MQ=255)
                                                                                                             tctgatctgaTGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCt                                >  3:276036/1‑149 (MQ=255)
                                                                                                             tctgatctgaTGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCt                                >  1:77393/1‑149 (MQ=255)
                                                                                                               tgatctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCttt                              <  1:61091/149‑1 (MQ=255)
                                                                                                                 atctgatGTATCTAACAGCCCCGCCCTATGCCGGTGCTGTTTCTTTTCGGCGATCGGTTACAGTTTGAGCCGGCGCTGGGCTTAGCGTCGCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACACGCTTtc                             >  5:270162/1‑148 (MQ=255)
                                                                                                                 atctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCttt                              <  2:65195/147‑1 (MQ=255)
                                                                                                                 atctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCtt                               <  5:323561/146‑1 (MQ=255)
                                                                                                                     gatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatcg                        >  3:230261/1‑149 (MQ=255)
                                                                                                                      atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatcga                       <  4:276036/149‑1 (MQ=255)
                                                                                                                      atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgat                          <  2:69580/146‑1 (MQ=255)
                                                                                                                              aaCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACt                 >  2:250569/1‑147 (MQ=255)
                                                                                                                               aCAGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAg              >  2:202765/1‑149 (MQ=255)
                                                                                                                               aCAGCCCCAACATAGGCCAAGGCTGTTTCTTTTCAGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTACGGTg                                                                                 >  8:47564/1‑82 (MQ=255)
                                                                                                                                cAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAg              >  2:152089/1‑148 (MQ=255)
                                                                                                                                cAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAg              >  1:224565/1‑148 (MQ=255)
                                                                                                                                 aGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACg                                                                                           <  7:13877/70‑1 (MQ=255)
                                                                                                                                 aGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACg                                                                                           >  8:13877/1‑70 (MQ=255)
                                                                                                                                  gCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTaa           >  4:111443/1‑149 (MQ=255)
                                                                                                                                  gCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTa            >  4:106854/1‑148 (MQ=255)
                                                                                                                                   ccccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGa               <  6:194959/144‑1 (MQ=255)
                                                                                                                                   ccccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGa               >  5:194959/1‑144 (MQ=255)
                                                                                                                                      cGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCa       <  6:7845/149‑1 (MQ=255)
                                                                                                                                             tGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTgg  <  3:183316/147‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
CTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGG  >  NC_002947/325927‑326214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑