| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,761,713 | G→T | 10.9% | G354V (GGG→GTG) | PP_3325 → | ferric siderophore receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,761,713 | 0 | G | T | 10.9% | 148.3 / 9.2 | 55 | G354V (GGG→GTG) | PP_3325 | ferric siderophore receptor |
| Reads supporting (aligned to +/- strand): ref base G (24/25); new base T (3/3); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-01 | |||||||||||
CTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAGCCA > NC_002947/3761568‑3761861 | cTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACggg > 5:81365/1‑147 (MQ=255) gcgcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGt > 2:72736/1‑149 (MQ=255) gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc > 7:290477/1‑149 (MQ=255) gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc > 3:30617/1‑149 (MQ=255) ccAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCggcctg < 1:121047/149‑6 (MQ=255) cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTgg > 2:217295/1‑149 (MQ=255) cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGACCTgg > 7:270663/1‑149 (MQ=255) aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCCCCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTg < 3:4603/146‑1 (MQ=255) aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGCGCTGCACGGGCAGTTCGAGCTg > 4:4603/1‑146 (MQ=255) cACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtgg > 6:105419/1‑149 (MQ=255) aCTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggc > 8:204138/1‑149 (MQ=255) cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc < 3:1106/149‑1 (MQ=255) cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc < 6:81365/149‑1 (MQ=255) acCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgca < 2:66060/149‑1 (MQ=255) ccAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcag > 2:99361/1‑148 (MQ=255) cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga > 2:231089/1‑139 (MQ=38) cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga < 1:231089/140‑2 (MQ=38) aGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCAt < 1:99361/149‑1 (MQ=255) gggCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATc < 4:117218/149‑1 (MQ=255) gCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGAccc < 8:290477/149‑1 (MQ=255) aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg < 1:72736/149‑1 (MQ=255) aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg < 4:231460/149‑1 (MQ=255) aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtgggg < 7:204138/149‑3 (MQ=255) gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGc < 4:3912/110‑1 (MQ=255) gggCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGc > 3:3912/1‑110 (MQ=255) cTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAAGGTGGACCGACAACATCACCCAGGCCGAGCTGCACGGGATGTTCGCGGTGGGTGGCTGGCAGCAACCGCCCCTGGTGGGGGTg > 1:258054/1‑147 (MQ=255) gTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAg > 5:38636/1‑149 (MQ=255) caccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAg > 1:216265/1‑148 (MQ=255) ccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAc < 8:270663/149‑1 (MQ=255) cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc < 2:133694/144‑1 (MQ=255) cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc > 1:133694/1‑144 (MQ=255) gcTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAgc < 5:105419/149‑1 (MQ=255) cTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCa > 7:47553/1‑149 (MQ=255) tCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGt > 3:202847/1‑149 (MQ=255) gcgAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTc < 4:74946/148‑1 (MQ=255) gcgACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCg > 8:163039/1‑149 (MQ=255) gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg > 1:317189/1‑66 (MQ=255) gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg < 2:317189/66‑1 (MQ=255) gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa > 5:20336/1‑149 (MQ=255) gACTCCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa > 2:89168/1‑149 (MQ=255) ttCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGGGCTGGGTGGCTGGCAGCATCAGCGCCTGGTGGGGCTGGAGTACGAACACTACCGCGACAGCCAGAAGTACCCGCACAGCGCCCCGTCCGCAGCGTATgg > 8:211661/1‑149 (MQ=255) cGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGc > 8:59350/1‑149 (MQ=255) a‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTgg < 1:89168/149‑1 (MQ=255) aCGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATc < 4:202847/149‑1 (MQ=255) acaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAAc < 1:217295/149‑1 (MQ=255) tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg > 4:107244/1‑69 (MQ=255) tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg < 3:107244/69‑1 (MQ=255) cACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCgg > 8:14469/1‑149 (MQ=38) ccAGGGCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGta > 7:291050/1‑148 (MQ=255) aGGCCGCGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATAc < 7:211661/149‑1 (MQ=255) ggCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACg > 1:105531/1‑149 (MQ=255) aGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAg < 4:30617/149‑1 (MQ=255) cTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACTCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGc < 8:47553/148‑1 (MQ=255) cACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCg < 7:59350/147‑1 (MQ=255) cACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGa < 2:105531/148‑1 (MQ=255) aCGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAag < 8:291050/149‑1 (MQ=255) ggCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAagcca < 6:20336/149‑1 (MQ=255) | CTGCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAGCCA > NC_002947/3761568‑3761861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |