Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,761,718 T→C 7.5% F356L (TTC→CTC)  PP_3325 → ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,761,7180TC 7.5% 150.3 / 2.6 53F356L (TTC→CTC) PP_3325ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (23/26);  new base C (2/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

GCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAGCCA  >  NC_002947/3761570‑3761861
                                                                                                                                                      |                                                                                                                                               
gcgcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGt                                                                                                                                                 >  2:72736/1‑149 (MQ=255)
  gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                               >  7:290477/1‑149 (MQ=255)
  gcCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTc                                                                                                                                               >  3:30617/1‑149 (MQ=255)
        ccAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCggcctg                                                                                                                                         <  1:121047/149‑6 (MQ=255)
         cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTgg                                                                                                                                        >  2:217295/1‑149 (MQ=255)
         cAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGACCTgg                                                                                                                                        >  7:270663/1‑149 (MQ=255)
           aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCCCCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTg                                                                                                                                         <  3:4603/146‑1 (MQ=255)
           aCCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGCGCTGCACGGGCAGTTCGAGCTg                                                                                                                                         >  4:4603/1‑146 (MQ=255)
             cACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtgg                                                                                                                                    >  6:105419/1‑149 (MQ=255)
              aCTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggc                                                                                                                                   >  8:204138/1‑149 (MQ=255)
                  cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc                                                                                                                               <  6:81365/149‑1 (MQ=255)
                  cacCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctggc                                                                                                                               <  3:1106/149‑1 (MQ=255)
                   acCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgca                                                                                                                              <  2:66060/149‑1 (MQ=255)
                     ccAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGgcag                                                                                                                             >  2:99361/1‑148 (MQ=255)
                      cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga                                                                                                                                    >  2:231089/1‑139 (MQ=38)
                      cAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGtga                                                                                                                                    <  1:231089/140‑2 (MQ=38)
                       aGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACAACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCAt                                                                                                                          <  1:99361/149‑1 (MQ=255)
                        gggCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATc                                                                                                                         <  4:117218/149‑1 (MQ=255)
                              gCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGAccc                                                                                                                   <  8:290477/149‑1 (MQ=255)
                                   aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg                                                                                                              <  4:231460/149‑1 (MQ=255)
                                   aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtggtg                                                                                                              <  1:72736/149‑1 (MQ=255)
                                   aGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCtgggg                                                                                                              <  7:204138/149‑3 (MQ=255)
                                             cTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAAGGTGGACCGACAACATCACCCAGGCCGAGCTGCACGGGATGTTCGCGGTGGGTGGCTGGCAGCAACCGCCCCTGGTGGGGGTg                                                                                                        >  1:258054/1‑147 (MQ=255)
                                                                gTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAg                                                                                 >  5:38636/1‑149 (MQ=255)
                                                                        caccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAg                                                                          >  1:216265/1‑148 (MQ=255)
                                                                          ccaTCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAc                                                                       <  8:270663/149‑1 (MQ=255)
                                                                                cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc                                                                      >  1:133694/1‑144 (MQ=255)
                                                                                cgcgCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTAcc                                                                      <  2:133694/144‑1 (MQ=255)
                                                                                   gcTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAgc                                                              <  5:105419/149‑1 (MQ=255)
                                                                                       cTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCa                                                          >  7:47553/1‑149 (MQ=255)
                                                                                          tCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGt                                                       >  3:202847/1‑149 (MQ=255)
                                                                                            gcgAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTc                                                      <  4:74946/148‑1 (MQ=255)
                                                                                                  gcgACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCg                                               >  8:163039/1‑149 (MQ=255)
                                                                                                    gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                                <  2:317189/66‑1 (MQ=255)
                                                                                                    gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGtggctgg                                                                                                                                >  1:317189/1‑66 (MQ=255)
                                                                                                    gACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa                                             >  5:20336/1‑149 (MQ=255)
                                                                                                    gACTCCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTa                                             >  2:89168/1‑149 (MQ=255)
                                                                                                       ttCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGGGCTGGGTGGCTGGCAGCATCAGCGCCTGGTGGGGCTGGAGTACGAACACTACCGCGACAGCCAGAAGTACCCGCACAGCGCCCCGTCCGCAGCGTATgg                                          >  8:211661/1‑149 (MQ=255)
                                                                                                         cGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGc                                        >  8:59350/1‑149 (MQ=255)
                                                                                                            a‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTgg                                     <  1:89168/149‑1 (MQ=255)
                                                                                                                   aCGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATc                                <  4:202847/149‑1 (MQ=255)
                                                                                                                         acaTCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAAc                          <  1:217295/149‑1 (MQ=255)
                                                                                                                            tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg                                                                                                       >  4:107244/1‑69 (MQ=255)
                                                                                                                            tCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTgg                                                                                                       <  3:107244/69‑1 (MQ=255)
                                                                                                                             cACCCAGCTCGAACTGCCCGTGCAGCTCGGCCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCgg                      >  8:14469/1‑149 (MQ=38)
                                                                                                                                ccAGGGCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGta                    >  7:291050/1‑148 (MQ=255)
                                                                                                                                  aGGCCGCGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATAc                 <  7:211661/149‑1 (MQ=255)
                                                                                                                                   ggCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACg                >  1:105531/1‑149 (MQ=255)
                                                                                                                                        aGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAg           <  4:30617/149‑1 (MQ=255)
                                                                                                                                          cTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACTCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGc          <  8:47553/148‑1 (MQ=255)
                                                                                                                                             cACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCg        <  7:59350/147‑1 (MQ=255)
                                                                                                                                             cACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGa       <  2:105531/148‑1 (MQ=255)
                                                                                                                                              aCGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAag     <  8:291050/149‑1 (MQ=255)
                                                                                                                                                 ggCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAagcca  <  6:20336/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                               
GCGCCTGGCCAACCACTACACCCAGGGCACGCTCAAGGGCAACAGCTCCGAGCCCCAGGCCCTGGTGAGCACCACCATCACGCGCTTCTATCGCGAACGCGACTTCGAA‑‑TGGAACGACAACATCACCCAGGCCGAGCTGCACGGGCAGTTCGAGCTGGGTGGCTGGCAGCATCAGACCCTGGTGGGGCTGGAGTACGAAAACTACCGCAACAGCCAGAAGTACCCGCAAAGCGCCACGTCCGCAGCGTATGGGCTGGATATCTACAACCCGGTATACGGCAAGCCGAAGCCA  >  NC_002947/3761570‑3761861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑