Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,781,955 T→A 7.1% M277K (ATG→AAG)  nikB → nickel ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,781,9550TA 7.1% 159.5 / 3.2 56M277K (ATG→AAG) nikBnickel ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (28/24);  new base A (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

GCCTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCATTGCT  >  NC_002947/3781813‑3782098
                                                                                                                                              |                                                                                                                                               
gCCTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTg                                                                                                                                           >  1:291690/1‑149 (MQ=255)
gCCTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGAAGCACTg                                                                                                                                           >  6:33928/1‑149 (MQ=255)
 ccTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGt                                                                                                                                            >  1:118117/1‑147 (MQ=255)
 ccTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGt                                                                                                                                            <  2:118117/147‑1 (MQ=255)
  cTGGCTGCCGCTGGTGCCGGCCACCGGCCTGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATg                                                                                                                                                >  3:10996/1‑142 (MQ=255)
  cTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATg                                                                                                                                                <  4:10996/142‑1 (MQ=255)
     gCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCa                                                                                                                                      <  5:228029/149‑1 (MQ=255)
         ccGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCAcc                                                                                                                                    <  3:173236/147‑1 (MQ=255)
         ccGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCAcc                                                                                                                                    >  4:173236/1‑147 (MQ=255)
         ccGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGAAGCACTGCATCAccgg                                                                                                                                  <  5:223777/149‑3 (MQ=255)
         ccGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCCGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGAAGCACTGCATCAcc                                                                                                                                    <  5:33928/147‑1 (MQ=255)
           gCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTg                                                                                                                                 >  8:1724/1‑148 (MQ=255)
                  aCGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCTGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGAc                                                                                                                         >  8:291575/1‑149 (MQ=255)
                           ggCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGt                                                                                                                                            >  2:235747/1‑121 (MQ=255)
                             cATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCtga                                                                                                              <  2:118211/149‑1 (MQ=255)
                                  acaTCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATc                                                                                                         >  2:101389/1‑149 (MQ=255)
                                    aTCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCt                                                                                                        >  2:146502/1‑148 (MQ=255)
                                    aTCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCt                                                                                                        <  1:146502/148‑1 (MQ=255)
                                         cGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCTGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCCGATGATCTGTAAcc                                                                                                  <  7:291575/149‑1 (MQ=255)
                                                cTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGt                                                                                           >  6:206526/1‑149 (MQ=255)
                                                   ggcggcGCACTGGTGCTCGAGACGCTCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGCCTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGCTCTGTAACCTGATAGTc                                                                                          >  1:282213/1‑147 (MQ=255)
                                                   ggcggcGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGa                                                                                        <  2:291690/149‑1 (MQ=255)
                                                   ggcggcGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGGTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGa                                                                                        >  1:277606/1‑149 (MQ=255)
                                                                  aTCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTg                                                                         >  3:298617/1‑149 (MQ=255)
                                                                   tCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTgg                                                                        >  4:168460/1‑149 (MQ=255)
                                                                     gagaCGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCt                                                                      >  8:136700/1‑149 (MQ=255)
                                                                        aCGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCt                                                                      <  1:266226/146‑1 (MQ=255)
                                                                        aCGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCt                                                                      >  2:266226/1‑146 (MQ=255)
                                                                             cTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTc                                                              >  5:90626/1‑149 (MQ=255)
                                                                                cGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTc                                                              <  5:206526/146‑1 (MQ=255)
                                                                                  ccTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGc                                                         <  7:1724/149‑1 (MQ=255)
                                                                                             gTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAgg                                              <  6:90626/149‑1 (MQ=255)
                                                                                              ttGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAggg                                             <  7:136700/149‑1 (MQ=255)
                                                                                                 gTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGt                                           >  8:9429/1‑148 (MQ=255)
                                                                                                 gTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGt                                           <  3:168460/148‑1 (MQ=255)
                                                                                                   cGTGTTGCGGTGTCGGCAGGGCGTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGaa                                        <  2:282213/149‑1 (MQ=255)
                                                                                                    ggtttGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGaa                                        <  2:277606/146‑1 (MQ=255)
                                                                                                     ttttGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCt                                                    <  5:331681/135‑1 (MQ=255)
                                                                                                     ttttGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCt                                                    >  6:331681/1‑135 (MQ=255)
                                                                                                       ttGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGc                                     <  3:172239/148‑1 (MQ=255)
                                                                                                       ttGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGc                                     <  1:101389/148‑1 (MQ=255)
                                                                                                          cGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGc                                     <  2:310894/145‑1 (MQ=255)
                                                                                                          cGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGc                                     >  1:310894/1‑145 (MQ=255)
                                                                                                          cGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCAt                                   >  5:28165/1‑147 (MQ=255)
                                                                                                             tgtCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGt                                           <  7:113569/136‑1 (MQ=255)
                                                                                                             tgtCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGt                                           >  8:113569/1‑136 (MQ=255)
                                                                                                                 ggCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGa                                 <  2:18099/142‑1 (MQ=255)
                                                                                                                 ggCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGa                                 >  1:18099/1‑142 (MQ=255)
                                                                                                                            aCCGCGACTTCCCGGTGATGCAGTGCTTCACCTTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGACTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGc               >  3:166656/1‑149 (MQ=255)
                                                                                                                            aCCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGc               <  4:298617/149‑1 (MQ=255)
                                                                                                                             ccGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCg              >  3:123434/1‑149 (MQ=255)
                                                                                                                             ccGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCg              >  7:181770/1‑149 (MQ=255)
                                                                                                                                   cTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGaa                                        <  2:313577/117‑1 (MQ=255)
                                                                                                                                   cTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGaa                                        >  1:313577/1‑117 (MQ=255)
                                                                                                                                      cagggTGAAGCACTGCATCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCAtt     >  7:186237/4‑149 (MQ=255)
                                                                                                                                         ggTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCATtgct  >  4:77024/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                               
GCCTGGCTGCCGCTGGTGACGGCCACCGGCATGCACATCGGCGAACTGCTCGGCGGCGCACTGGTGATCGAGACGATCTTCGCCTGGCCCGGCGTTGGTCGTTTTGCGGTGTCGGCAGTGCTTAACCGCGACTTCCCGGTGATGCAGTGCTTCACCCTGCTACTGACCGGCCTGCTGATGATCTGTAACCTGATAGTCGATGTGTGCTACGCCTGGCTTGATCCTCGCAGCCGGCTGGAAGGGGTGAACGCATGAGTGTGCACGCTGCCCTGCGTCAGCCATTGCT  >  NC_002947/3781813‑3782098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑