Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,798,704 A→T 11.1% W66R (TGG→AGG)  PP_3358 ← hydroxycinnamoyl‑CoA hydratase‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,798,7040AT11.1% 116.1 / 6.4 45W66R (TGG→AGG) PP_3358hydroxycinnamoyl‑CoA hydratase‑lyase
Reads supporting (aligned to +/- strand):  ref base A (19/21);  new base T (3/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.41e-01

CCGTTGACCATGGCGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAGGCGATGCCCGCT  >  NC_002947/3798556‑3798852
                                                                                                                                                     |                                                                                                                                                    
ccGTTGACCATGGCGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCa                                                                                                                                                      <  4:225534/149‑1 (MQ=255)
 cGTTGACCATGGCGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAg                                                                                                                                                     <  5:304440/149‑1 (MQ=255)
   ttGACCATGGCGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGa                                                                                                                                                   <  3:66902/149‑1 (MQ=255)
       ccATGGCGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCg                                                                                                                                               >  7:15875/1‑149 (MQ=255)
             cGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCGAGTCCTGGAC‑CGCCGGCa                                                                                                                                         <  5:39679/149‑1 (MQ=255)
                 ggTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCgg                                                                                                                                     >  4:85830/1‑149 (MQ=255)
                           gCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCa                                                                                                                            <  8:283694/148‑1 (MQ=255)
                             aTACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCGAGTCCTGGAC‑CGCCGGCa                                                                                                                                         >  5:3197/1‑133 (MQ=255)
                             aTACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCGAGTCCTGGAC‑CGCCGGCa                                                                                                                                         <  6:3197/133‑1 (MQ=255)
                             aTACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCa                                                                                                                         >  7:172013/1‑149 (MQ=255)
                             aTACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCa                                                                                                                         >  8:199052/1‑149 (MQ=255)
                                        gcaACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCcagcgtca                                                                                                              <  8:15875/149‑1 (MQ=255)
                                             ttCCATTGCCATTGCGAGGCTTCGCTACTAATCTTTTCCTTGAGTATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCTTGCCGGCG‑GTCCAGGACTCTCCGGCACCTGTAAATACCAGCACGCCagcttcagcgtc                                                                                                         <  8:257640/149‑1 (MQ=255)
                                             ttCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCcagcgtca                                                                                                              >  2:117770/1‑144 (MQ=255)
                                             ttCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCcagcgtca                                                                                                              <  1:117770/144‑1 (MQ=255)
                                                cattgccattgcGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTg                                                                                                      <  8:250437/149‑1 (MQ=255)
                                                   tgccattgcGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCt                                                                                                    >  4:136652/1‑148 (MQ=255)
                                                     ccattgcGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTc                                                                                                   >  7:146925/1‑147 (MQ=255)
                                                     ccattgcGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTc                                                                                                   >  1:79457/1‑147 (MQ=255)
                                                       attgcGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAgg                                                                                               >  2:174433/1‑149 (MQ=255)
                                                         tgcGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCATTACCAGCACGCCAGCGTCAGGGTCCTGCTCAAgg                                                                                               <  3:14342/147‑1 (MQ=255)
                                                            gAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTc                                                                                          <  3:85830/149‑1 (MQ=255)
                                                               gCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAg                                                                                       >  8:155823/1‑149 (MQ=255)
                                                                        cgaATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGCAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGATTCCAGCACgacgac                                                                              >  1:21540/1‑149 (MQ=255)
                                                                          attCTTTTCGTGGTGGATGGCCGGGCCGGCTTCCAGCTCGCGGAAGTACTCCTTCAGGTCCATGGCGGCG‑GTCCAGGACTCGCCGTCTCCTGTCAATACCAGCTCGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGAcaa                                                                            <  8:277379/147‑3 (MQ=255)
                                                                              ttttCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTc                                                                        <  8:172013/149‑1 (MQ=255)
                                                                                 tCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTccc                                                                      <  7:155823/148‑1 (MQ=255)
                                                                                  ccTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCgg                                                                    >  6:82231/1‑149 (MQ=255)
                                                                                    tGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGt                                                                   >  6:216466/1‑148 (MQ=255)
                                                                                      gaggaTTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCgg                                                                    >  4:224151/1‑145 (MQ=255)
                                                                                      gaggaTTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCgg                                                                    <  3:224151/145‑1 (MQ=255)
                                                                                        ggaTTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCa                                                                                                                            >  1:243038/1‑87 (MQ=255)
                                                                                        ggaTTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCa                                                                                                                            <  2:243038/87‑1 (MQ=255)
                                                                                           tttCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAgg                                                              >  3:27011/1‑146 (MQ=255)
                                                                                           tttCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTACATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAgg                                                              <  4:27011/146‑1 (MQ=255)
                                                                                                   ccGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCAt                                                   >  7:12253/1‑149 (MQ=255)
                                                                                                     ggCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGTACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATgg                                                 <  8:146925/149‑1 (MQ=255)
                                                                                                     ggCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATgg                                                 <  4:120220/149‑1 (MQ=255)
                                                                                                                  cgGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCGAGGAGTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTGAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTc                                    <  2:21540/149‑1 (MQ=255)
                                                                                                                   ggAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTcc                                   <  8:12253/149‑1 (MQ=255)
                                                                                                                          cTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGa                             <  5:216466/148‑1 (MQ=255)
                                                                                                                                       gtGCCGGCGAGTCCTGGAC‑CGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGttt                                       >  4:40104/2‑125 (MQ=255)
                                                                                                                                       gtGCCGGCGAGTCCTGGAC‑CGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTGTCCAGCACGTCGCCCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTcccc                 >  7:115051/2‑147 (MQ=255)
                                                                                                                                         gCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAgg             >  5:231183/1‑149 (MQ=255)
                                                                                                                                             gcg‑gTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAGGCGAt         <  2:79457/149‑1 (MQ=255)
                                                                                                                                                    cAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCCGGGGGGGGCTCCTGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAGGCGATGCCCGc   >  3:46626/1‑149 (MQ=255)
                                                                                                                                                     aGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAGGCGATGCCCGCt  >  1:230150/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGTTGACCATGGCGATGGTCGGTTTGGCATACAGACGCAGCAACTTCCATTGCCATTGCGAGGCTTCGCGACGAATCTTTTCCTGGAGGATTTCCGGGCCGGCGTCCACCTCGCGGAAGTACTCCTTCAGGTCCATGCCGGCG‑GTCCAGGACTCGCCGGCACCGGTCAATACCAGCACGCCAGCGTCAGCGTCCTGCTCAAGGGTTTCCAGCACGTCGACCATTTCCCGGTTCAGGGTGGGGCTCATGGCATTGCGTTTTTCCGGGCGATTGAGGGTCACCCAGGCGATGCCCGCT  >  NC_002947/3798556‑3798852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑