Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,811,859 T→C 14.7% Y61Y (TAT→TAC)  PP_3368 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,811,8590TC14.7% 77.7 / 6.4 34Y61Y (TAT→TAC) PP_3368MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (17/12);  new base C (3/2);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

CCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTGGCCTGGT  >  NC_002947/3811723‑3812002
                                                                                                                                        |                                                                                                                                               
ccAAGAGGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCATACGCGCTgatggca                                                                                                                                     <  7:180412/149‑7 (MQ=255)
     aTGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCATACGCGCTgatg                                                                                                                                        <  8:40107/141‑4 (MQ=255)
     aTGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCATACGCGCTgatg                                                                                                                                        >  7:40107/1‑138 (MQ=255)
                    gCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGTGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACt                                                                                                                 >  3:180933/1‑149 (MQ=255)
                     cACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTg                                                                                                                >  1:296893/1‑149 (MQ=255)
                     cACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTg                                                                                                                >  8:57177/1‑149 (MQ=255)
                               cTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCAATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCg                                                                                                      >  4:237460/1‑149 (MQ=255)
                                 ttGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGt                                                                                                    >  4:162549/1‑149 (MQ=255)
                                     cATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGc                                                                                                  >  4:270976/1‑147 (MQ=255)
                                     cATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGc                                                                                                  <  3:270976/147‑1 (MQ=255)
                                      aTCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCa                                                                                                >  5:295172/1‑148 (MQ=255)
                                              cGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGTGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACtgctgc                                                                                       <  3:18083/149‑1 (MQ=255)
                                                gCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTgcgc                                                                                     <  5:216500/149‑1 (MQ=255)
                                                gCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTgcg                                                                                      >  6:216500/1‑148 (MQ=255)
                                                            tcatGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACAtgctg                                                                         >  7:59859/1‑149 (MQ=255)
                                                              atGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGccc                                                                                                                        >  2:308645/1‑100 (MQ=255)
                                                              atGGGCCTGATGCCCGACATTGCCAGCAATTCGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGccc                                                                                                                        <  1:308645/100‑1 (MQ=255)
                                                                       aTGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGAt                                                              >  3:227001/1‑149 (MQ=255)
                                                                               cATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGt                                                      <  7:57177/149‑1 (MQ=255)
                                                                                             tGCAGTTGAGCGAACCGCAGGTGTGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCaa                                         <  4:180933/148‑1 (MQ=255)
                                                                                                      gCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACt                               <  6:295172/149‑1 (MQ=255)
                                                                                                        gAACCGCAGGTGGGACATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATACTCGGTGCCAAACTGCTACGCAACCACATGCTGCTCCTGTTGATGCTGCTGTATGCCGTGGGCAACCTCGCCACTGc                             >  4:204110/1‑149 (MQ=255)
                                                                                                          aCCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGcc                            >  2:303114/1‑148 (MQ=255)
                                                                                                          aCCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGcc                            <  1:303114/148‑1 (MQ=255)
                                                                                                          aCCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACATGGCCACTGCCt                           >  8:299544/1‑149 (MQ=255)
                                                                                                            cGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCttt                         >  3:199206/1‑149 (MQ=255)
                                                                                                                 gTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGcccc                    <  2:296893/149‑1 (MQ=255)
                                                                                                                         tgccatCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCg            >  4:74630/1‑149 (MQ=255)
                                                                                                                          gccatCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCt                                      <  7:3207/122‑1 (MQ=255)
                                                                                                                          gccatCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCt                                      >  8:3207/1‑122 (MQ=255)
                                                                                                                            ccccAGCGCATACGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTg         >  3:295738/4‑149 (MQ=255)
                                                                                                                             cccAGCGCATACGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTgg        >  1:267377/3‑149 (MQ=255)
                                                                                                                              tCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTGGc       <  7:299544/149‑1 (MQ=255)
                                                                                                                                   gcgTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTTCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTGGCCTGGt  <  3:204110/149‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                               
CCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTTGAGCGAACCGCAGGTGGGCCATGCCATCAGCGCGTATGCGCTGGGGGTAATGGTTGGCGCCCCGACACTGGCCATTCTCGGTGCCAAACTGCTGCGCAAGCACATGCTGCTGCTGTTGATGCTGCTGTATGCCGTGGGCAACCTGGCCACTGCCTTTGCCCCCTCGTTCGGTGGCCTGGT  >  NC_002947/3811723‑3812002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑