Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,912,563 C→A 5.2% C303* (TGC→TGA) ‡ PP_3452 → GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,912,5630CA 5.2% 250.2 / 2.3 77C303* (TGC→TGA) ‡PP_3452GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (38/35);  new base A (2/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

TGGGCGACGAAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGCAAGGACCGCTTT  >  NC_002947/3912415‑3912710
                                                                                                                                                    |                                                                                                                                                   
tGGGCGACGAAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGc                                                                                                                                                     <  2:79338/149‑1 (MQ=255)
  ggCGACGAAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGc                                                                                                                                                     >  1:315461/1‑147 (MQ=255)
    cgacgaAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAgc                                                                                                                                                 >  5:9164/1‑149 (MQ=255)
     gacgaAGTGCTCAAGCGCGTGGCCCTACATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAgcg                                                                                                                                                <  2:184457/149‑1 (MQ=255)
       cgaAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGcgcg                                                                                                                                              >  7:28710/1‑149 (MQ=255)
            tGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTgcgc                                                                                                                                         <  8:80373/149‑1 (MQ=255)
               tCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAg                                                                                                                                      >  4:95024/1‑149 (MQ=255)
                 aaGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAgg                                                                                                                                     <  7:102070/148‑1 (MQ=255)
                  aGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGGCACAGcgcgg                                                                                                                                             <  5:161395/139‑2 (MQ=255)
                  aGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGGCACAGcgcgg                                                                                                                                             >  6:161395/1‑138 (MQ=255)
                   gcgcGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCGGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGATGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACg                                                                                                                                  >  7:54602/1‑149 (MQ=255)
                                   atCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGt                                                                                                                  >  8:124931/1‑149 (MQ=255)
                                    tCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGt                                                                                                                  >  4:52321/1‑148 (MQ=255)
                                    tCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGt                                                                                                                  >  7:94700/1‑148 (MQ=255)
                                    tCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTc                                                                                                                 >  8:257611/1‑149 (MQ=255)
                                     cAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCa                                                                                                                >  6:183147/1‑149 (MQ=255)
                                      aaGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCAc                                                                                                               <  2:89218/149‑1 (MQ=255)
                                      aaGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCAc                                                                                                               >  3:192558/1‑149 (MQ=255)
                                       aGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCAcc                                                                                                              <  7:257611/149‑1 (MQ=255)
                                       aGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCAc                                                                                                               >  8:19124/1‑148 (MQ=255)
                                        gACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCAcc                                                                                                              >  2:114884/1‑148 (MQ=255)
                                         aCAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCAGTCACCAt                                                                                                            <  7:21019/149‑1 (MQ=255)
                                           aaGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCa                                                                                                          <  5:183147/149‑1 (MQ=255)
                                             gCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAg                                                                                                         >  7:17074/1‑148 (MQ=255)
                                                tGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCAtt                                                                                                     <  1:114884/149‑1 (MQ=255)
                                                tGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCAtt                                                                                                     <  6:305588/149‑1 (MQ=255)
                                                  gCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtgg                                                                                                   <  1:121023/149‑1 (MQ=255)
                                                  gCCGATCAGGCGTTAGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtgg                                                                                                   >  8:263366/1‑149 (MQ=255)
                                                   ccGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggc                                                                                                  <  4:311231/149‑1 (MQ=255)
                                                      aTCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggcctg                                                                                               <  7:263366/149‑1 (MQ=255)
                                                      aTCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggcctg                                                                                               <  4:192558/149‑1 (MQ=255)
                                                      aTCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggcctg                                                                                               <  5:73343/149‑1 (MQ=255)
                                                      aTCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggcctg                                                                                               <  3:95024/149‑1 (MQ=255)
                                                      aTCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggcctg                                                                                               <  6:21480/149‑1 (MQ=255)
                                                       tCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCa                                                                                                          >  1:108774/1‑137 (MQ=255)
                                                       tCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCa                                                                                                          <  2:108774/137‑1 (MQ=255)
                                                             gTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAgg                                                                                        >  2:222715/1‑149 (MQ=255)
                                                                 gCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGGCGCTGGCGAGCACCCGGTGCCCGTCAGCATTGGCCTGGCCAAGGTCGa                                                                                    >  8:237107/1‑149 (MQ=255)
                                                                   ccGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCg                                                                                                                            <  4:69118/107‑1 (MQ=255)
                                                                   ccGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCg                                                                                                                            >  3:69118/1‑107 (MQ=255)
                                                                    cGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATc                                                                                                           <  5:310736/123‑1 (MQ=255)
                                                                    cGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATc                                                                                                           >  6:310736/1‑123 (MQ=255)
                                                                           gAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGGCGACCGGGga                                                                             >  6:110201/1‑146 (MQ=255)
                                                                                gAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCt                                                                     >  4:234817/1‑149 (MQ=255)
                                                                                gAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCt                                                                     >  3:33951/1‑149 (MQ=255)
                                                                                gAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCt                                                                     >  7:121560/1‑149 (MQ=255)
                                                                                  agagTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTa                                                                   <  7:124931/149‑1 (MQ=255)
                                                                                     gTTCGCGATCTTCATCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAACATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTacca                                                                <  2:298466/149‑1 (MQ=255)
                                                                                      ttCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTaccac                                                               >  5:296563/1‑149 (MQ=255)
                                                                                         gcgATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCAt                                                                                                            <  5:157383/101‑1 (MQ=255)
                                                                                         gcgATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCAt                                                                                                            >  6:157383/1‑101 (MQ=255)
                                                                                         gcgATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACcgc                                                            >  6:117063/1‑149 (MQ=255)
                                                                                               ttcttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCtt                                                                    >  4:269215/1‑135 (MQ=255)
                                                                                               ttcttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCtt                                                                    <  3:269215/135‑1 (MQ=255)
                                                                                                tcttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTc                                                     >  8:167684/1‑149 (MQ=255)
                                                                                                 cttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCgcgc                                                          <  4:33951/143‑1 (MQ=255)
                                                                                                 cttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGAGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTcc                                                    >  1:43654/1‑149 (MQ=255)
                                                                                                   tcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCcgc                                                  >  2:196971/1‑149 (MQ=255)
                                                                                                    cGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCAGCGCggtggtaagg                                                  <  6:9164/148‑5 (MQ=255)
                                                                                                     gAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCgcgc                                                <  2:315461/149‑1 (MQ=255)
                                                                                                      aaGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGTGAGAGAGCCCTACCACCGCGCTGGTCCGCGcc                                               <  8:17074/149‑1 (MQ=255)
                                                                                                      aaGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGcc                                               <  3:234817/149‑1 (MQ=255)
                                                                                                           cTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCa                                          <  8:121560/149‑1 (MQ=255)
                                                                                                              gATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACcgcg                                                           <  3:175127/129‑1 (MQ=255)
                                                                                                              gATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACcgcg                                                           >  4:175127/1‑129 (MQ=255)
                                                                                                              gATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAgg                                        >  2:271112/1‑148 (MQ=255)
                                                                                                                    cAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATtata                                 <  5:110201/149‑1 (MQ=255)
                                                                                                                         ggCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAg                            >  8:34895/1‑149 (MQ=255)
                                                                                                                         ggCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAg                            >  2:15741/1‑149 (MQ=255)
                                                                                                                             gAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCaa                        <  8:94700/149‑1 (MQ=255)
                                                                                                                              agcagcGAGTGGCAGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAg                       <  8:54602/149‑1 (MQ=255)
                                                                                                                               gcagcGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCAGGGAGAGAGCCTTACCAGCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGc                      >  5:276755/1‑149 (MQ=255)
                                                                                                                                cagcGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGca                     <  2:43654/149‑1 (MQ=255)
                                                                                                                                cagcGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGca                     >  6:36957/1‑149 (MQ=255)
                                                                                                                                      gTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTgg               >  2:204681/1‑149 (MQ=255)
                                                                                                                                        gcacgcgCTGGCACAGCGCGTGCGCTAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCaa                        >  8:131329/4‑138 (MQ=255)
                                                                                                                                        gcacgcgCTGGCACAGCGCGTGCGCTAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCaa                        <  7:131329/135‑1 (MQ=255)
                                                                                                                                         gCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGc              >  2:106271/1‑147 (MQ=255)
                                                                                                                                           cgcgCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGCAAgg          <  1:222715/149‑1 (MQ=255)
                                                                                                                                                    ccAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGCAAGGACCGCttt  <  7:19124/148‑1 (MQ=255)
                                                                                                                                                    ccAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGCAAGGACCGCttt  <  1:106271/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGGCGACGAAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGCAAGGACCGCTTT  >  NC_002947/3912415‑3912710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑