Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,932,041 C→G 9.2% G479A (GGC→GCC)  PP_3466 ← ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,932,0410CG 9.2% 159.5 / 5.9 54G479A (GGC→GCC) PP_3466ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (24/25);  new base G (3/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

ACAGCTGGTCGATGGCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACG  >  NC_002947/3931895‑3932183
                                                                                                                                                   |                                                                                                                                              
aCAGCTGGTCGATGGCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCcg                                                                                                                                              >  2:248216/1‑149 (MQ=255)
  aGCTGGTCGATGGCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCcgcg                                                                                                                                            <  3:292707/149‑1 (MQ=255)
          gATGGCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCa                                                                                                                                    <  2:2813/149‑1 (MQ=255)
            tGGCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAg                                                                                                                                   >  6:30037/1‑148 (MQ=255)
                cgcgATCACTTCACTCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGc                                                                                                                              <  4:302279/149‑1 (MQ=255)
                    aTCACTTCCCCCTCGGTGGCCTCATCCACTGCCGAACTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGATCGCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCAt                                                                                                                           <  1:83834/149‑1 (MQ=255)
                        cTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAgg                                                                                                                      >  3:215732/1‑149 (MQ=255)
                           cACGCTCGGTGGCCTCATCCACTGCCGCAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGc                                                                                                                     <  4:1299/147‑1 (MQ=255)
                           cACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGcc                                                                                                                    <  5:30037/148‑1 (MQ=255)
                           cACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGc                                                                                                                     <  8:295141/147‑1 (MQ=255)
                              gCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGTATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGc                                                                                                                >  1:233219/1‑149 (MQ=255)
                              gCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGGCGAGCGCGGCCAGCagttgt                                                                                                                            <  5:176253/137‑6 (MQ=255)
                              gCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGGCGAGCGCGGCCAGCagttgt                                                                                                                            >  6:176253/1‑132 (MQ=255)
                              gCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGc                                                                                                                     <  5:42928/144‑1 (MQ=255)
                              gCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGc                                                                                                                     >  6:42928/1‑144 (MQ=255)
                                        cTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGt                                                                                                      >  5:29471/1‑149 (MQ=255)
                                         tCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCACGCCATCCAGGCCCAGCGGCAGGCTGTc                                                                                                     >  1:303532/1‑149 (MQ=255)
                                         tCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTc                                                                                                     >  7:510/1‑149 (MQ=255)
                                           aTCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTc                                                                                                     <  2:245904/147‑1 (MQ=255)
                                              cACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCa                                                                                                <  2:55329/149‑1 (MQ=255)
                                                   ccGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGAACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCCGGCTg                                                                                           >  7:113111/1‑149 (MQ=255)
                                                    cGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGt                                                                                          <  5:144417/149‑1 (MQ=255)
                                                      aaGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTcc                                                                                        <  8:510/149‑1 (MQ=255)
                                                      aaGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTc                                                                                         >  3:65718/1‑148 (MQ=255)
                                                         tGGCCTCGTCCAGCGCCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGCCTGTCCAGc                                                                                     >  5:43328/1‑149 (MQ=255)
                                                            ccTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCcgc                                                                                  >  7:195465/1‑149 (MQ=255)
                                                             cTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCcgcg                                                                                 >  7:277804/1‑149 (MQ=255)
                                                                gTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCgcgc                                                                                <  6:29471/147‑1 (MQ=255)
                                                                        ccAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGcacc                                                                      >  5:329103/1‑149 (MQ=255)
                                                                        ccAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGcac                                                                       >  5:329105/1‑148 (MQ=255)
                                                                         cAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGcacca                                                                     >  5:119714/1‑149 (MQ=255)
                                                                          aGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGGGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGcaccac                                                                    <  6:43328/149‑1 (MQ=255)
                                                                          aGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGcaccac                                                                    <  3:190400/149‑1 (MQ=255)
                                                                                            aaCACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTc                                                                                         >  4:292948/1‑110 (MQ=255)
                                                                                            aaCACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTc                                                                                         <  3:292948/110‑1 (MQ=255)
                                                                                                cTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCgcgc                                                                                <  5:328498/115‑1 (MQ=255)
                                                                                                cTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCgcgc                                                                                >  6:328498/1‑115 (MQ=255)
                                                                                                  gCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGc                                            >  2:29860/1‑149 (MQ=255)
                                                                                                   cACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGc                                            >  4:189907/1‑148 (MQ=255)
                                                                                                       aGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCgg                                       <  4:65718/149‑1 (MQ=255)
                                                                                                                gAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCa                               >  2:173327/1‑148 (MQ=255)
                                                                                                                gAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCAt                              <  1:173327/149‑1 (MQ=255)
                                                                                                                       ttgttTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGc                          >  6:90076/1‑147 (MQ=255)
                                                                                                                       ttgttTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCa                        <  3:189907/149‑1 (MQ=255)
                                                                                                                              tGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTg                 >  5:207783/1‑149 (MQ=255)
                                                                                                                                gCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTgc                <  2:233219/148‑1 (MQ=255)
                                                                                                                                gCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTg                 <  8:277804/147‑1 (MQ=255)
                                                                                                                                  ccccGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTc                                                              <  1:181115/100‑1 (MQ=255)
                                                                                                                                  ccccGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTc                                                              >  2:181115/1‑100 (MQ=255)
                                                                                                                                     cGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAgg          <  6:329105/149‑1 (MQ=255)
                                                                                                                                     cGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAgg          <  6:329103/149‑1 (MQ=255)
                                                                                                                                          gcttgctgGGCGAGCGCGGCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCa                               >  2:202709/4‑123 (MQ=255)
                                                                                                                                          gcttgctgGGCGAGCGCGGCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCa                               <  1:202709/120‑1 (MQ=255)
                                                                                                                                             tgctgGGCGAGCGCGGCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACg  >  3:28733/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
ACAGCTGGTCGATGGCCGCGATCACTTCACGCTCGGTGGCCTCATCCACTGCCGAAGTGGCCTCGTCCAGCACCAGGATCGCCGGGGCCTGCAACACTGCACGAGCAATGGCGAT‑GCGTTGTTTCTGGCCCCCGGACAACTGCTGGCCGCGCTCGCCCAGCAAGCCATCCAGGCCCAGCGGCAGGCTGTCGACCAGGCTGTCCAGCCGCGCCAGGCGCACCACCCGTTCCAGTTCATCACGGCTGGCCTCGGGCACGCCATAAGCCAGGTTCTGCGCCAGGGTGCCACG  >  NC_002947/3931895‑3932183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑