Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,942,965 T→C 5.5% T310A (ACC→GCC)  PP_3478 ← secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,942,9650TC 5.5% 222.2 / 2.5 73T310A (ACC→GCC) PP_3478secretion protein
Reads supporting (aligned to +/- strand):  ref base T (35/34);  new base C (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

ATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGGTAGTCCT  >  NC_002947/3942827‑3943093
                                                                                                                                          |                                                                                                                                
aatGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGtt                                                                                                                         >  5:118957/3‑148 (MQ=255)
aTTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTg                                                                                                                        <  6:324204/149‑1 (MQ=255)
 ttGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGtt                                                                                                                         >  6:154261/1‑147 (MQ=255)
    cAGGCCCAGGGCGAGGCTGCGCGTGGGGTCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGgc                                                                                                                    <  4:72182/149‑1 (MQ=255)
         cAAGGTCGAGGATGCGCGAGGTGGCCACCTACATAACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGAGACTCCAGCAGCTTCTCGGCCATGCGAACCCCGTCCGACGTGTCGCGCATCGTCAGGGTGTTGAGGCGCTCg                                                                                                               >  5:281796/1‑149 (MQ=255)
                        gcgAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTcg                                                                                                >  7:57609/1‑149 (MQ=255)
                        gcgAGGTGGCCACCTCCATCACCTACACATCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGAGCTCGTCAACGAACACGTcg                                                                                                >  1:179287/1‑149 (MQ=255)
                         cgAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTcgc                                                                                               >  7:183413/1‑149 (MQ=255)
                           aGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCgg                                                                                             <  4:133077/149‑1 (MQ=255)
                            ggTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGa                                                                                                                       <  6:265038/122‑1 (MQ=255)
                            ggTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGa                                                                                                                       >  5:265038/1‑122 (MQ=255)
                                gCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCtt                                                                                        >  4:20407/1‑149 (MQ=255)
                                  cACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCtt                                                                                        >  5:267157/1‑147 (MQ=255)
                                  cACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGa                                                                                      <  2:179287/149‑1 (MQ=255)
                                   aCCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAg                                                                                     <  3:20407/149‑1 (MQ=255)
                                     cTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCa                                                                                   >  6:205043/1‑149 (MQ=255)
                                     cTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCa                                                                                   <  2:254814/149‑1 (MQ=255)
                                     cTCCATCACCTACACTTCCAGCACCACCTCGGGGTTGGACTTGTCCTGAGACTGCAGCAGCTTCTCGGACATGCGCACCTACTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTAGTCAACGAACACGTCGCGGATCttaaac                                                                                    >  6:318781/1‑144 (MQ=255)
                                       ccATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATg                                                                                 >  6:243749/1‑149 (MQ=255)
                                       ccATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATg                                                                                 >  2:271940/1‑149 (MQ=255)
                                            aCCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAgg                                                                                                                              <  2:66084/99‑1 (MQ=255)
                                            aCCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAgg                                                                                                                              >  1:66084/1‑99 (MQ=255)
                                            aCCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCtt                                                                            <  5:243749/149‑1 (MQ=255)
                                            aCCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAACATGGTc                                                                              >  7:172197/1‑147 (MQ=255)
                                              cTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                          >  6:294990/1‑149 (MQ=255)
                                              cTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                          >  8:44987/1‑149 (MQ=255)
                                               tCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGaa                                                                                                       >  1:295110/1‑119 (MQ=255)
                                               tCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGaa                                                                                                       <  2:295110/119‑1 (MQ=255)
                                               tCCACTTCCAGCACCACCTCGGGGTTAGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTcaagac                                                                         >  2:107282/1‑144 (MQ=255)
                                                 cACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGAcc                                                                        <  6:181952/148‑1 (MQ=255)
                                                 cACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGAc                                                                         <  6:267157/147‑1 (MQ=255)
                                                    ttCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGt                                                                    >  5:47505/1‑149 (MQ=255)
                                                     tCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGt                                                                    >  1:82572/1‑148 (MQ=255)
                                                       cAGCACCACGTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTACGACTTGTCGCGCATGGTCAGTGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAACATGGTCAAGACCATGCTCa                                                                 <  5:318781/149‑1 (MQ=255)
                                                       cAGCACCACCTCGGGGTTGGACTGGTCGTGCGACTGCAGCATCTTCTCGGCCATGCGCACCGCGTCCGAGGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAAGGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTc                                                                  <  6:281796/148‑1 (MQ=255)
                                                        aGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAACATGGTCAAGACCATGCTCaa                                                                <  8:172197/149‑1 (MQ=255)
                                                         gCACCACCTCGGGGTTAGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCAAGACCATGCTCaag                                                               <  1:107282/149‑2 (MQ=255)
                                                           accaccTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAAcgc                                                             >  1:98373/1‑149 (MQ=255)
                                                            ccaccTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAAcgcg                                                            >  2:14839/1‑149 (MQ=255)
                                                             caccTCGGGGTTTGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCgg                                                           <  1:303332/149‑1 (MQ=255)
                                                              accTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGgt                                                          >  8:206054/1‑149 (MQ=255)
                                                              accTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGgt                                                          <  8:19464/149‑1 (MQ=255)
                                                              accTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGgt                                                          >  6:126141/1‑149 (MQ=255)
                                                               ccTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGgtg                                                         >  4:94216/1‑149 (MQ=255)
                                                                cTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGgtgt                                                        >  5:234472/1‑149 (MQ=255)
                                                                   ggggTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCAGCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGttttt                                                      <  7:206054/148‑2 (MQ=255)
                                                                   ggggTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGtt                                                       <  6:234472/147‑1 (MQ=255)
                                                                    gggTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGc                                                    <  3:187867/149‑1 (MQ=255)
                                                                      gTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGt                                                  <  5:294990/149‑1 (MQ=255)
                                                                               gTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTgg                                         >  5:93072/1‑149 (MQ=255)
                                                                               gTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTgg                                         <  7:44987/149‑1 (MQ=255)
                                                                                tCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCa                                                                                                            >  3:19220/1‑81 (MQ=255)
                                                                                tCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCa                                                                                                            <  4:19220/81‑1 (MQ=255)
                                                                                         tGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGCCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGt                                                  >  1:139907/1‑130 (MQ=255)
                                                                                         tGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGCCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGt                                                  >  1:144744/1‑130 (MQ=255)
                                                                                         tGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGCCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGt                                                  <  2:139907/130‑1 (MQ=255)
                                                                                         tGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGCCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGt                                                  <  2:144744/130‑1 (MQ=255)
                                                                                           cagcagCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAAc                              <  3:94216/148‑1 (MQ=255)
                                                                                             gcagcTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTg                           >  8:82509/1‑149 (MQ=255)
                                                                                              cagcTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTgc                          <  6:47505/149‑1 (MQ=255)
                                                                                                 cTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCa                       >  7:84348/1‑149 (MQ=255)
                                                                                                  ttCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAgg                                    <  1:32360/135‑1 (MQ=255)
                                                                                                  ttCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAgg                                    >  2:32360/1‑135 (MQ=255)
                                                                                                  ttCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCAt                      >  7:267264/1‑149 (MQ=255)
                                                                                                    ctcGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTg                                          >  3:186802/1‑127 (MQ=255)
                                                                                                    ctcGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTg                                          <  4:186802/127‑1 (MQ=255)
                                                                                                        gCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                          >  7:273923/1‑91 (MQ=255)
                                                                                                        gCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGa                                                                          <  8:273923/91‑1 (MQ=255)
                                                                                                           aTGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTc             <  6:93072/149‑1 (MQ=255)
                                                                                                           aTGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTc             <  5:205043/149‑1 (MQ=255)
                                                                                                               gcACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTgg         <  6:118957/149‑1 (MQ=255)
                                                                                                                      gTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGGTAGTCCt  <  2:82572/149‑1 (MQ=255)
                                                                                                                      gTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGGTAGTCCt  <  2:98373/149‑1 (MQ=255)
                                                                                                                                          tCAGGGTGTTGAGGCGCTCGTCAACTAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGAc                   <  3:25823/112‑1 (MQ=255)
                                                                                                                                          tCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCCGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGAc                   >  4:25823/1‑112 (MQ=255)
                                                                                                                                          |                                                                                                                                
ATTGCAGGCCAAGGTCGAGGATGCGCGAGGTGGCCACCTCCATCACCTCCACTTCCAGCACCACCTCGGGGTTGGACTGGTCCTGCGACTGCAGCAGCTTCTCGGCCATGCGCACCGCGTCCGACGTGTCGCGCATGGTCAGGGTGTTGAGGCGCTCGTCAACGAACACGTCGCGGGTCTTGAGCATGGTCTTGACCATGTTCAACGCGGTGTTGGCGTCGATGCTGGTCAGGTAGAACGTGCGCATGACCAGTTCCTGGTAGTCCT  >  NC_002947/3942827‑3943093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑