Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,996,352 C→T 11.6% intergenic (+30/+15) PP_3523 → / ← PP_3524 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,996,3520CT11.6% 134.9 / 5.3 51intergenic (+30/+15)PP_3523/PP_3524hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (19/26);  new base T (3/3);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCATGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGATCCACAGCATCTTGCGCTCGGCGCGCCGTT  >  NC_002947/3996212‑3996494
                                                                                                                                            |                                                                                                                                              
aTCCATGACGCGCTCACGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCGGATGGTGGACGGGCGGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCGGGCGCGGGGGCGCCTGAGGCCgt                                                                                                                                        <  4:22032/149‑2 (MQ=255)
  ccATGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCa                                                                                                                                       >  5:224433/1‑148 (MQ=255)
        cgcgCTCACGGCACTGCGCGTACTGTCCACCGCGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTc                                                                                                                                <  6:224433/149‑1 (MQ=255)
             tCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCGGGCGCGGGGGCGCCTGAGGcc                                                                                                                                          <  8:288673/134‑1 (MQ=255)
             tCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCGGGCGCGGGGGCGCCTGAGGcc                                                                                                                                          >  7:288673/1‑134 (MQ=255)
               aaGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTc                                                                                                                         <  2:236101/149‑1 (MQ=255)
                   aaCTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGc                                                                                                                     <  4:156663/149‑1 (MQ=255)
                               tGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCgccctg                                                                                                         >  3:176400/1‑149 (MQ=255)
                                   cACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCgccctgccct                                                                                                     >  2:280855/1‑149 (MQ=255)
                                      cGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCa                                                                                                  >  1:13928/1‑149 (MQ=255)
                                      cGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCa                                                                                                  <  3:180519/149‑1 (MQ=255)
                                        tGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCAcc                                                                                                <  1:55933/149‑1 (MQ=255)
                                        tGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCAcc                                                                                                >  3:230191/1‑149 (MQ=255)
                                             ttCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCACACGCGCCCCCCCCCCCCACGCCCCAAACCGGCAATTTTACGGCAACCCCGCCCAGCCCTTCCCCGcccg                                                                                           >  2:309304/1‑148 (MQ=255)
                                                tACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCCACCCCGCCCAGCCCTTCCCCGCCCTCgg                                                                                        >  2:272912/1‑149 (MQ=255)
                                                  cGGCTGCCAGATGGTGGACGGGCTGGGTCCTGCCATCCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCACCCGCACCCGCACCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTACAACGCCCTCGCAc                                                                                      >  4:101083/1‑149 (MQ=255)
                                                            aTGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCAttttt                                                                                                                          <  5:103777/103‑1 (MQ=255)
                                                            aTGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCAttttt                                                                                                                          >  6:103777/1‑103 (MQ=255)
                                                            aTGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGc                                                                            <  2:255244/149‑1 (MQ=255)
                                                               gtggACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCa                                                                         <  5:35893/149‑1 (MQ=255)
                                                                    cGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACa                                                                    <  3:145521/149‑1 (MQ=255)
                                                                             tCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAATAGCAAACCgg                                                           <  1:280855/149‑1 (MQ=255)
                                                                              ccTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCggg                                                          <  3:304006/149‑1 (MQ=255)
                                                                                tGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCCCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGCACAGCAAACCcggg                                                         >  8:219483/1‑148 (MQ=255)
                                                                                  ccATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAg                                                      >  1:122553/1‑149 (MQ=255)
                                                                                  ccATGCGTTTCGACATCATGCCCACATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGaa                                                       <  8:111354/148‑1 (MQ=255)
                                                                                   cATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAgg                                                     <  2:122553/149‑1 (MQ=255)
                                                                                     tGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCCAACCGGGGAAGGGt                                                   >  6:154087/1‑149 (MQ=255)
                                                                                      gCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCgccctgccc                                                                                                      <  3:110941/97‑1 (MQ=255)
                                                                                      gCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCgccctgccc                                                                                                      >  4:110941/1‑97 (MQ=255)
                                                                                       cGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGc                                                 <  2:13928/149‑1 (MQ=255)
                                                                                        gTTTCGCCATCCTGCCCAAATGCCCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCCTTTTTCCTGCACCACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGACCAGCAAACCGGGGACGGGTGCg                                                >  6:257199/1‑149 (MQ=255)
                                                                                        gTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCCCCCTGCCCTTAACCGCCCTCGGACCGCGCCAGGCCCAGAACACAAAACCCGGGAAGGGTGCg                                                >  6:19436/1‑149 (MQ=255)
                                                                                         tttCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGaa                                                       <  1:84981/141‑1 (MQ=255)
                                                                                         tttCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGAAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGaa                                                       >  2:84981/1‑141 (MQ=255)
                                                                                             gACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGAc                                           <  3:101083/149‑1 (MQ=255)
                                                                                               cATCCTGCCCATATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGCGTGCGTAGACCa                                         <  3:271691/149‑1 (MQ=255)
                                                                                                 tCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACg                                       <  4:309196/149‑1 (MQ=255)
                                                                                                  ccTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCgg                                                           <  6:112120/128‑1 (MQ=255)
                                                                                                  ccTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCgg                                                           >  5:112120/1‑128 (MQ=255)
                                                                                                  ccTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCAc                                        <  3:312479/147‑1 (MQ=255)
                                                                                                  ccTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCAc                                        >  4:312479/1‑147 (MQ=255)
                                                                                                   cTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGaa                                     <  5:107364/149‑1 (MQ=255)
                                                                                                          aaTGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGATc                              <  1:309304/149‑1 (MQ=255)
                                                                                                           aTGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGATcc                             <  5:19436/149‑1 (MQ=255)
                                                                                                                   ttgcGGCCTCGGGCGCTGGGGCGCCTGAGGCCGTAAACCGTCATTTTTCCTGAAACACCGCCCTCCCCTACACCGCCCTCGGACCGCGCCAGGCCCAGAACACCAAACCGGCGAATGGTGCGTAGACCACGAAGACGATCCACAGCaac                     >  1:319244/4‑147 (MQ=255)
                                                                                                                     gcGGCCTCGGGCGCGGGGGCGCCTGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCa                                                                                                  <  3:108121/69‑1 (MQ=18)
                                                                                                                     gcGGCCTCGGGCGCGGGGGCGCCTGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCa                                                                                                  >  4:108121/2‑70 (MQ=18)
                                                                                                                           tCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGATCCACAGCATCTTGCGCTc             >  5:21303/1‑149 (MQ=255)
                                                                                                                              ggCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGAGCCACAGCATCTTGCGCTCGgc          <  5:154087/149‑1 (MQ=255)
                                                                                                                                      cgcgACCGAGGCCGCAAACCGTCATTTTTCCTTCTACACCGCCCTGCCCTTCACCGACCTCGGACCGCTCCAGCCCCAGAACACAAAAGCGGGGAAGTGTGCGTAGACCACTAATACGATCCACAGCATCTTGCGCTCGGCGCGCCGtt  <  2:265760/149‑1 (MQ=255)
                                                                                                                                          cccGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGATCCACAGCATCTTGCGCTCGgcgcgc      <  7:127218/141‑1 (MQ=255)
                                                                                                                                          cccGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGATCCACAGCATCTTGCGCTCGgcgcgc      >  8:127218/1‑141 (MQ=255)
                                                                                                                                            cGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGATCCACAGCATCTTGCGCTCGgcgc        >  7:247519/1‑137 (MQ=255)
                                                                                                                                            cGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGATCCACAGCATCTTGCGCTCGgcgc        <  8:247519/137‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                              
ATCCATGACGCGCTCAAGGAACTGCGCGTACTGTCCACCGTGAAGTTCTACGGCTGCCTGATGGTGGACGGGCTGGGTCCTGCCATGCGTTTCGACATCCTGCCCAAATGACCCTCGACGGCCTCAGGCGCCCCCGCGCCCGAGGCCGCAAACCGTCATTTTTCCTGCAACACCGCCCTGCCCTTCACCGCCCTCGGACCGCGCCAGGCCCAGAACAGCAAACCGGGGAAGGGTGCGTAGACCACGAAGACGATCCACAGCATCTTGCGCTCGGCGCGCCGTT  >  NC_002947/3996212‑3996494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑