| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,999,697 | G→C | 13.8% | V128L (GTG→CTG) | PP_3528 → | ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,999,697 | 0 | G | C | 13.8% | 91.8 / 4.1 | 34 | V128L (GTG→CTG) | PP_3528 | ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (11/18); new base C (2/2); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCTACTGGAGGCATTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTGCAGCAGGCTGGGCTCAGTTACAAGGACATTACCCCCGCATACGTC > NC_002947/3999555‑3999843 | ccAGCTACTGGAGGCATTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCCTGctcg > 2:50968/1‑149 (MQ=255) gCTACTGGAGGCATTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGc < 4:79470/149‑1 (MQ=255) gCTACTGGAGGCATTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGc < 2:150019/149‑1 (MQ=255) cTACTGGAGGCAGTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCa < 5:110707/149‑1 (MQ=255) aGGCATTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGaccac < 2:247151/149‑1 (MQ=255) aGGCATTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCCTGCTCGCGCACGaccag < 1:50968/149‑2 (MQ=255) ggCATTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGaccacc < 5:59312/149‑1 (MQ=255) ggCATTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGaccac < 2:287071/148‑1 (MQ=255) cGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCCTGCTCGCGCACGaccag > 8:179727/1‑135 (MQ=255) cGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCCTGCTCGCGCACGaccag < 7:179727/136‑2 (MQ=255) cATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGAcc > 5:309918/1‑149 (MQ=255) aCCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGaaa < 3:24071/149‑1 (MQ=255) gCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCgtgt > 2:283811/1‑149 (MQ=255) cAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGtgtg < 2:242707/149‑1 (MQ=255) cAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGtgtg < 4:113906/149‑1 (MQ=255) cAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGtgtg > 1:53066/1‑149 (MQ=255) aGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTgg > 7:271175/1‑149 (MQ=255) cccAGGCCGCAGGCAAGCCTGT‑GTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGcc > 2:69089/1‑148 (MQ=255) cAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCa < 2:103826/149‑1 (MQ=255) cGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTAcc > 8:59490/1‑149 (MQ=255) gCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACct < 8:271175/149‑1 (MQ=255) ggCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACctgct < 1:283811/149‑1 (MQ=255) cAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTgg < 6:66378/149‑1 (MQ=255) ggTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGccc > 8:54931/1‑149 (MQ=255) aCCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTgcag > 5:70773/1‑149 (MQ=255) ccTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTgca < 6:309918/147‑1 (MQ=255) cTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCATGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTgcag > 2:261455/1‑147 (MQ=255) caacCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTGCAGCAGGCTGGGCTCAg < 7:54931/149‑1 (MQ=255) aacCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTgca < 6:182301/133‑1 (MQ=255) aacCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTgca > 5:182301/1‑133 (MQ=255) ccACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTGCAGCAGGCTGGGCTCAGTTACAAGGa < 6:70773/149‑1 (MQ=255) gCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTGCAGCAGGCTGGGCTCAGTTACAAGGACATTACCCCCGCa > 5:71246/1‑149 (MQ=255) cTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTGCAGCAGGCTGGGCTCAGTTACAAGGACATTACCCCCGCATa < 7:59490/149‑1 (MQ=255) cGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTGCAGCAGGCTGGGCTCAGTTACAAGGACATTACCCCCGCATACGTc > 5:192444/1‑148 (MQ=255) | CCAGCTACTGGAGGCATTGAGCACCGGCGCCATCGACCTCGGCCACGCAGCCGATGCCCCCTCGGTGTTCGCCCAGGCCGCAGGCAAGCCTGTGGTCTACCTGGCTGCCGAACAACCCTACCCACGCGGCATCGGCCTGGTCGTGCGCGAGCAGGACCACCTGGCCAGTGTGCAGGACCTGAAAGGCAAGCGTGTGGCCACGGGCCGCGGCTGGAATGCCCAGTACCTGCTGGCCGTAGCCCTGCAGCAGGCTGGGCTCAGTTACAAGGACATTACCCCCGCATACGTC > NC_002947/3999555‑3999843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |