Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,100,584 A→G 6.6% E153G (GAA→GGA)  PP_3608 → LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,100,5840AG 6.6% 175.2 / 2.4 60E153G (GAA→GGA) PP_3608LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (22/34);  new base G (2/2);  total (24/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

CGCCGGTTCCGGCTGTCGCTGTCCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTGGCGATATGGCCCTGGAAGACTGGCT  >  NC_002947/4100436‑4100722
                                                                                                                                                    |                                                                                                                                          
cgcCGGTTCCGGCTGTCGCTGTCCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGa                                                                                                                                            <  2:272656/149‑1 (MQ=255)
 gcCGGTTCCGGCTGTCGCTGTCCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGaa                                                                                                                                           <  6:173168/149‑1 (MQ=255)
     gTTCCGGCTGTCGCTGTCCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTgg                                                                                                                                       <  5:58234/149‑1 (MQ=255)
       tCCGGCTGTCGCTGTCCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAAc                                                                                                                                          >  3:229347/1‑144 (MQ=255)
       tCCGGCTGTCGCTGTCCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAAc                                                                                                                                          <  4:229347/144‑1 (MQ=255)
         cGGCTGTCGCTGTCCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCt                                                                                                                                   <  1:232856/149‑1 (MQ=255)
           gctgtcgctgtcCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTgg                                                                                                                                 <  6:299026/149‑1 (MQ=255)
           gctgtcgctgtcCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTgg                                                                                                                                 <  7:195176/149‑1 (MQ=255)
           gctgtcgctgtcCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTgg                                                                                                                                 <  3:142621/149‑1 (MQ=255)
                     tcCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGc                                                                                                                         <  2:76486/147‑1 (MQ=255)
                     tcCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGc                                                                                                                         <  4:278288/147‑1 (MQ=255)
                     tcCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGc                                                                                                                         <  8:103110/147‑1 (MQ=255)
                       cGACTACGCTTCGCGCATCATCCTGCCGCACCGGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTg                                                                                                                      <  3:29084/148‑1 (MQ=255)
                            aCGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTcccc                                                                                                                >  1:9391/1‑149 (MQ=255)
                             cGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTcccc                                                                                                                <  2:9391/148‑1 (MQ=255)
                                   gcgcATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGaa                                                                                                                                           >  6:276004/1‑115 (MQ=255)
                                   gcgcATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGc                                                                                                         >  7:274456/1‑149 (MQ=255)
                                   gcgcATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGc                                                                                                         <  3:155052/149‑1 (MQ=255)
                                       atcatcCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCACGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCt                                                                                                       >  4:8022/1‑147 (MQ=255)
                                        tcatcCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAg                                                                                                    <  8:274456/149‑1 (MQ=255)
                                             cTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACAt                                                                                               <  6:24241/149‑1 (MQ=255)
                                               gCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCa                                                                                             >  5:256024/1‑149 (MQ=255)
                                                        gTTGCGCCACCTGCGCCAAGTCGCACCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCa                                                                                      >  8:258589/1‑147 (MQ=255)
                                                        gTTGCGCCACCTGCGCCAAGTCGCACCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCa                                                                                      <  7:258589/147‑1 (MQ=255)
                                                          tGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGccc                                                                                  >  4:211859/1‑149 (MQ=255)
                                                              ccACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCg                                                                                          >  5:73455/1‑137 (MQ=255)
                                                              ccACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCg                                                                                          <  6:73455/137‑1 (MQ=255)
                                                                  cTGCGCCAAGTCGCGCCCGGGGTGGTCCTGGCCATCAGCCCGGCTAGCCGGGAAACCATGTTGGCGCAATTGCTCGATGGCGAAGTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGc                                                                          <  6:235847/149‑1 (MQ=255)
                                                                  cTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGc                                                                          <  6:332341/149‑1 (MQ=255)
                                                                    gcgcCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGcc                                                                         >  3:111193/1‑148 (MQ=255)
                                                                      gcCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGAt                                                                      >  2:199936/1‑149 (MQ=255)
                                                                               cgcCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTtcat                                                              >  1:69268/1‑148 (MQ=255)
                                                                                gcCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTtcatca                                                            <  6:256024/149‑1 (MQ=255)
                                                                                  ccGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGGACTGGATCTGGCCCTGGGCCTGTTCCCCGAAGTGCCTCAgg                                                                                                   <  2:171709/108‑1 (MQ=255)
                                                                                  ccGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGGACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAgg                                                                                                   >  1:171709/1‑108 (MQ=255)
                                                                                  ccGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGGACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAgg                                                                                                   >  7:230578/1‑108 (MQ=255)
                                                                                  ccGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGGACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAgg                                                                                                   <  8:230578/108‑1 (MQ=255)
                                                                                   cGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGTCCCTGTTTGCCGATCGCTTCATCAGCg                                                         <  1:177823/149‑1 (MQ=255)
                                                                                       gTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCa                                                                                             <  3:118164/109‑1 (MQ=255)
                                                                                       gTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCa                                                                                             >  4:118164/1‑109 (MQ=255)
                                                                                       gTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGc                                                     <  2:69268/149‑1 (MQ=255)
                                                                                            ccTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATc                                                <  1:199936/149‑1 (MQ=255)
                                                                                                 ccATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGcc                                                                                   <  2:272567/109‑1 (MQ=255)
                                                                                                   aTCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAgga                                         >  6:127593/1‑148 (MQ=255)
                                                                                                     cAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATc                                                >  3:93091/1‑140 (MQ=255)
                                                                                                     cAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATc                                                <  4:93091/140‑1 (MQ=255)
                                                                                                      aGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTcct                                      >  5:162864/1‑149 (MQ=255)
                                                                                                          aggacagCCGTGAAACCATGTTCCCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCTCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTcctgcct                                  <  7:202545/149‑1 (MQ=255)
                                                                                                            gccagCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCctgcctgc                                <  6:162864/149‑1 (MQ=255)
                                                                                                               agCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAg                             <  3:211859/149‑1 (MQ=255)
                                                                                                                  cGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTgg                          <  3:8022/149‑1 (MQ=255)
                                                                                                                  cGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTCCCTCAGGACATCACAGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTgg                          >  4:263995/1‑149 (MQ=255)
                                                                                                                   gTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTGGc                         <  3:263995/149‑1 (MQ=255)
                                                                                                                    tGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTGGc                         >  8:115131/1‑148 (MQ=255)
                                                                                                                        aCCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTtc                                                                <  6:93970/105‑1 (MQ=255)
                                                                                                                        aCCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTtc                                                                >  5:93970/1‑105 (MQ=255)
                                                                                                                        aCCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTGGCGatat                    >  8:159629/1‑149 (MQ=255)
                                                                                                                          cATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTcctgcc                                   >  3:134442/1‑132 (MQ=255)
                                                                                                                          cATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTcctgcc                                   <  4:134442/132‑1 (MQ=255)
                                                                                                                           aTGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTGGCGATATGGc                 >  7:115090/1‑149 (MQ=255)
                                                                                                                                   gcAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTgg                          >  4:260991/1‑132 (MQ=255)
                                                                                                                                   gcAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTgg                          <  3:260991/132‑1 (MQ=255)
                                                                                                                                          cTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTGGCGATATGGCCCTGGAAGActggct  <  7:115131/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
CGCCGGTTCCGGCTGTCGCTGTCCGACTACGCTTCGCGCATCATCCTGCCGCACCTGTTGCGCCACCTGCGCCAAGTCGCGCCCGGGGTGGACCTGGCCATCAGCCAGGCCAGCCGTGAAACCATGTTGGCGCAATTGCTCGATGGCGAACTGGATCTGGCCCTGGGCCTGTTCCCCGAACTGCCTCAGGACATCACCGCCCAGCCCCTGTTTGCCGATCGCTTCATCAGCGTTGCCGATCGCCAGGTCCTGCCTGCCAGTGGCGATATGGCCCTGGAAGACTGGCT  >  NC_002947/4100436‑4100722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑