Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,103,403 T→G 7.0% intergenic (‑158/‑59) PP_3611 ← / → PP_3612 hypothetical protein/TonB‑dependent receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,103,4030TG 7.0% 177.6 / 5.0 57intergenic (‑158/‑59)PP_3611/PP_3612hypothetical protein/TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base T (24/29);  new base G (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

CCCTGTGATCAAGTCCGAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCCTGCTGTCCACGCTGCCGCCCAAACCGATAC  >  NC_002947/4103256‑4103550
                                                                                                                                                   |                                                                                                                                                   
cccTGTGATCAAGTCCGAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCtt                                                                                                                                                    >  3:93079/1‑149 (MQ=255)
 ccTGTGATCAAGTCCGAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCtt                                                                                                                                                    >  2:185441/1‑148 (MQ=255)
      gATCAAGTACGAATAGGCGCGTGAAAGGCTCACGCGCTGACAACCATAGCAATTACCGGCCGCCTTGACGGCCCTCTCGGCTGGAGACGCATATCGTCGCCGAAATCACTGATATAGCTAATAAGAATTCTTAACACTTGCTTTACAtt                                                                                                                                              >  6:285890/1‑149 (MQ=255)
        tCAAGTCCGAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACAttt                                                                                                                                             <  4:310272/148‑1 (MQ=255)
          aaGTCCGAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGc                                                                                                                                           >  1:23724/1‑148 (MQ=255)
            gTCCGAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCaaa                                                                                                                                        >  6:325468/1‑149 (MQ=255)
              ccGAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGACCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGc                                                                                                                                           <  3:117548/144‑1 (MQ=255)
              ccGAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGACCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGc                                                                                                                                           >  4:117548/1‑144 (MQ=255)
                gAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCAt                                                                                                                                    <  3:119479/149‑1 (MQ=255)
                         cgTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCg                                                                                                                           <  1:185441/149‑1 (MQ=255)
                          gTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGTCCCTCTCGGCTGGCGACGCATTTCGTCGCCTAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGt                                                                                                                          <  5:285890/149‑1 (MQ=255)
                          gTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGt                                                                                                                          <  2:62747/149‑1 (MQ=255)
                           tGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGt                                                                                                                          >  7:493/1‑148 (MQ=255)
                            gAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTa                                                                                                                                  >  8:76840/1‑139 (MQ=255)
                            gAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTa                                                                                                                                  <  7:76840/139‑1 (MQ=255)
                             aaaGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGa                                                                                                                        <  4:157631/148‑1 (MQ=255)
                              aaGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGt                                                                                                                          >  8:169132/1‑145 (MQ=255)
                              aaGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGt                                                                                                                          <  7:169132/145‑1 (MQ=255)
                                     aCGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGa                                                                                                                 <  3:226308/147‑1 (MQ=255)
                                     aCGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGa                                                                                                                 <  8:5333/147‑1 (MQ=255)
                                                 gCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGt                                                                                                   <  8:493/149‑1 (MQ=255)
                                                     aGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACAt                                                                                                                                               >  6:87691/1‑101 (MQ=255)
                                                            aCCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATg                                                                                        >  2:320666/1‑149 (MQ=255)
                                                             ccGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCg                                                                                                 <  8:123700/139‑1 (MQ=255)
                                                             ccGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCg                                                                                                 <  8:128022/139‑1 (MQ=255)
                                                             ccGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCg                                                                                                 >  7:128022/1‑139 (MQ=255)
                                                             ccGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCg                                                                                                 >  7:123700/1‑139 (MQ=255)
                                                             ccGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATgg                                                                                       >  7:113952/1‑149 (MQ=255)
                                                             ccGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATg                                                                                        <  4:92608/148‑1 (MQ=255)
                                                               ggCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGc                                                                                      >  5:116434/1‑148 (MQ=255)
                                                                     cTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTccc                                                                                                                   <  4:214704/113‑1 (MQ=255)
                                                                     cTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTccc                                                                                                                   >  3:214704/1‑113 (MQ=255)
                                                                        gCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGt                                                                              <  1:320666/147‑1 (MQ=255)
                                                                         ccGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCt                                                                            <  2:23724/148‑1 (MQ=255)
                                                                                    ggCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCa                                                                >  6:59167/1‑149 (MQ=255)
                                                                                       tGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCGTTACATTTGCAAATCATTACTATTCGt                                                                                                                          >  6:118028/1‑88 (MQ=255)
                                                                                       tGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCGTTACATTTGCAAATCATTACTATTCGt                                                                                                                          >  6:123095/1‑88 (MQ=255)
                                                                                       tGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCGTTACATTTGCAAATCATTACTATTCGt                                                                                                                          <  5:123095/88‑1 (MQ=255)
                                                                                       tGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCGTTACATTTGCAAATCATTACTATTCGt                                                                                                                          <  5:118028/88‑1 (MQ=255)
                                                                                           ctcGCATTTCGTCGCCGAAATCACTGTTATAGCAAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCAtt                                                          <  7:225169/146‑1 (MQ=255)
                                                                                              gCATTTCGTCGCCGAAATCACTGTTATAGCAAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGCCAAAAAGGTCTGTTGCCCCAGCATCCAtc                                                          >  8:225169/1‑144 (MQ=255)
                                                                                                aTTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATg                                                    <  4:93079/149‑1 (MQ=255)
                                                                                                     gtcgCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTggg                                               <  5:325468/149‑1 (MQ=255)
                                                                                                     gtcgCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTgg                                                <  4:295804/148‑1 (MQ=255)
                                                                                                        gCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCt                                            >  4:175679/1‑149 (MQ=255)
                                                                                                               tCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGtt                                     <  3:175679/149‑1 (MQ=255)
                                                                                                                   tGTTATAGCTAATACGAATTCTTTACACTTTCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCATGTAATGGAAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGc                                 >  4:169574/1‑149 (MQ=255)
                                                                                                                     ttATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCct                               >  5:223282/1‑149 (MQ=255)
                                                                                                                     ttATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCct                               >  3:154056/1‑149 (MQ=255)
                                                                                                                       ataGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTtgt                                   >  7:176534/1‑143 (MQ=255)
                                                                                                                       ataGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTtgt                                   <  8:176534/143‑1 (MQ=255)
                                                                                                                       ataGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCctgc                             >  7:240132/1‑149 (MQ=255)
                                                                                                                                  gAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCCTGCTGTCCACGCTg                  >  3:154067/1‑149 (MQ=255)
                                                                                                                                     ttCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCCTGCTGTCCACGCTgccg               <  4:154056/149‑1 (MQ=255)
                                                                                                                                         ttACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCAGTTCCATGCTGGGCGTGCTCGTTGTGCCTGCTGTCCACGCTGCCGccc            >  8:151934/1‑148 (MQ=255)
                                                                                                                                          tACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCCTGCTGTCCACGCTgccgcc             <  3:254321/146‑1 (MQ=255)
                                                                                                                                            cacTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCCTGCTGTCCACGCTGCCGCCCAAAc        <  8:113952/149‑1 (MQ=255)
                                                                                                                                                  cTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCCTGCTGTCCACGCTGCCGCCCAAACCGATAc  <  2:109928/149‑1 (MQ=255)
                                                                                                                                                  cTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCCTGCTGTCCACGCTGCCGCCCAAACCGATAc  <  8:240132/149‑1 (MQ=255)
                                                                                                                                                   tttACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCCTGCTGTCCACGCTGCCGCCCAAACCGATAc  >  3:199748/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCCTGTGATCAAGTCCGAATAGGCGCGTGAAAGGCTCACGCGCTGACAAGCATAGCCATTACCGGCCGCCTTGCCGGCCCTCTCGGCTGGCGACGCATTTCGTCGCCGAAATCACTGTTATAGCTAATACGAATTCTTTACACTTGCTTTACATTTGCAAATCATTACTATTCGTGACTCCCGATAACAACAAAGAGTCGCCTGTAATGGCAAAAAGGTCTGTTGCCCCAGCATCCATTTCCATGCTGGGCCTGCTCGTTGTGCCTGCTGTCCACGCTGCCGCCCAAACCGATAC  >  NC_002947/4103256‑4103550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑