Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,142,562 C→G 6.7% A232G (GCC→GGC)  PP_3646 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,142,5620CG 6.7% 195.8 / 4.0 60A232G (GCC→GGC) PP_3646aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (31/25);  new base G (2/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

CATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCC  >  NC_002947/4142422‑4142707
                                                                                                                                              |                                                                                                                                                 
cATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCAccg                                                                                                                                           >  6:260060/1‑149 (MQ=255)
   ccTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGGCGGCAccgccg                                                                                                                                        <  6:49744/149‑2 (MQ=255)
     tCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCCACGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGAGCGCCTTCACCGGCGGTGCCGCCAccgcccg                                                                                                                                       >  1:215785/1‑149 (MQ=255)
               ccGACTGGCCCTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTAc                                                                                                                            >  7:294775/1‑149 (MQ=255)
                cGACTGGCCCTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGTGGGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGATGGTGCGCAAGATCGCCTTCACCGGCGGTgc                                                                                                                                                   >  1:71693/1‑126 (MQ=255)
                cGACTGGCCCTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTgc                                                                                                                                                   <  2:71693/125‑1 (MQ=255)
                  aCTGGCCCTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACgca                                                                                                                         <  8:57528/149‑1 (MQ=255)
                       cccTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACgcagca                                                                                                                      >  6:139264/1‑147 (MQ=255)
                       cccTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACgcagca                                                                                                                      <  5:139264/147‑1 (MQ=255)
                         cTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAgcgc                                                                                                                  <  7:161070/149‑1 (MQ=255)
                               gCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGGCGGCAccgccg                                                                                                                                        <  6:319815/121‑2 (MQ=255)
                               gCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGGCGGCAccgccg                                                                                                                                        >  5:319815/1‑120 (MQ=255)
                               gCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGaaaa                                                                                                            >  2:32237/1‑149 (MQ=255)
                                 cGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCTCCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTTGTACGCAGCAGCGCCGa                                                                                                               <  5:164351/144‑1 (MQ=255)
                                 cGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGa                                                                                                               >  6:164351/1‑144 (MQ=255)
                                    cTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCg                                                                                                       <  3:198339/149‑1 (MQ=255)
                                          cggccggTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAg                                                                                                  >  6:158688/1‑149 (MQ=255)
                                            gccggTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCt                                                                                                <  7:140369/149‑1 (MQ=255)
                                                gtgtGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCg                                                                                            <  3:65292/149‑1 (MQ=255)
                                                     gTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGGAGGTGCGCAAGATCGCCTTCCCCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTgg                                                                                        >  1:249826/1‑148 (MQ=255)
                                                     gTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTgg                                                                                        >  8:275745/1‑148 (MQ=255)
                                                     gTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTgg                                                                                        <  6:24509/148‑1 (MQ=255)
                                                        aaCGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACt                                                                                    >  8:4218/1‑149 (MQ=255)
                                                          cGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTgg                                                                                  >  2:106273/1‑149 (MQ=255)
                                                          cGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTgg                                                                                  >  5:225987/1‑149 (MQ=255)
                                                              ggTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAAc                                                                                     >  1:217134/1‑143 (MQ=255)
                                                              ggTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAAc                                                                                     <  2:217134/143‑1 (MQ=255)
                                                                tCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGggcggc                                                                             <  6:225987/149‑1 (MQ=255)
                                                                       cTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGTTGTCGCTGGAACTGGGCGGCAAGTCGc                                                                      >  4:47437/1‑149 (MQ=255)
                                                                          cGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGa                                                                   <  5:98805/149‑1 (MQ=255)
                                                                            gCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAAc                                                                 <  7:4218/149‑1 (MQ=255)
                                                                             ccccAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAAc                                                                 >  4:309481/1‑148 (MQ=255)
                                                                                 aGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAAcatcat                                                            >  3:26751/1‑149 (MQ=255)
                                                                                  gCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACatcatc                                                           <  3:79361/149‑1 (MQ=255)
                                                                                     ccGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTc                                                        <  4:143547/149‑1 (MQ=255)
                                                                                             gcgcTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTcgccgacgc                                                >  1:78604/1‑149 (MQ=255)
                                                                                              cgcTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTcgccgacgcc                                               >  6:226229/1‑149 (MQ=255)
                                                                                                  gACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGt                                                                                                                                 >  2:272332/1‑63 (MQ=255)
                                                                                                  gACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGt                                                                                                                                 <  1:272332/63‑1 (MQ=255)
                                                                                                   aCCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCgccgacgccgac                                            <  7:275745/147‑1 (MQ=255)
                                                                                                    cccGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGt                                                                                              >  2:59925/1‑96 (MQ=255)
                                                                                                    cccGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGt                                                                                              <  1:59925/96‑1 (MQ=255)
                                                                                                     ccGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCTGCCACCGCCCGCCACGt                                                                                                                                 >  8:217290/1‑60 (MQ=255)
                                                                                                     ccGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCt                                                          >  8:29925/1‑131 (MQ=255)
                                                                                                     ccGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCt                                                          <  7:29925/131‑1 (MQ=255)
                                                                                                     ccGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTg                                         >  6:33646/1‑148 (MQ=255)
                                                                                                     ccGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTg                                         >  7:256839/1‑148 (MQ=255)
                                                                                                            ccGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAgcgc                                 >  3:20165/1‑149 (MQ=255)
                                                                                                             cGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGcc                                <  2:249826/149‑1 (MQ=255)
                                                                                                               cTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCAt                              <  4:26751/149‑1 (MQ=255)
                                                                                                                        aaGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGgcgc                     >  1:255230/1‑149 (MQ=255)
                                                                                                                          gATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTGCGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAAGTGGGCGGCAAGTCGCCGAACCTCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCg                   >  4:126839/1‑149 (MQ=255)
                                                                                                                          gATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCg                   >  2:211111/1‑149 (MQ=255)
                                                                                                                           aTCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCCGGACAGCGCCATCAATGGCGCCGt                  >  5:75409/1‑149 (MQ=255)
                                                                                                                              gCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTTCGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGc               <  8:256839/149‑1 (MQ=255)
                                                                                                                               ccTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGc               >  7:72189/1‑148 (MQ=255)
                                                                                                                                cTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCg             <  5:260060/149‑1 (MQ=255)
                                                                                                                                 gtggcgggcggTGGCGGCACCGCCGGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACCGCGCCATCAATGGCGCCGTGGCCgg            >  6:173294/7‑149 (MQ=255)
                                                                                                                                   cACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCgg            <  5:158688/147‑1 (MQ=255)
                                                                                                                                   cACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGc           <  2:215785/148‑1 (MQ=255)
                                                                                                                                          ggTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGc   <  3:309481/149‑1 (MQ=255)
                                                                                                                                           gTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGcc  >  4:139489/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
CATCCTCGAGCTGGCCCGACTGGCCCTTGAGGCCGGCTTCC‑CGGCCGGTGTGGTCAACGT‑GGTCACCGGCTACGGCCCCAGCACCGGCGCGGCGCTGACCCGCCACCCGCTGGTGCGCAAGATCGCCTTCACCGGCGGTGCCGCCACCGCCCGCCACGTGGTACGCAGCAGCGCCGAAAACTTCGCCAAGCTGTCGCTGGAACTGGGCGGCAAGTCGCCGAACATCATCTTCGCCGACGCCGACCTGGACAGCGCCATCAATGGCGCCGTGGCCGGCATCTATGCC  >  NC_002947/4142422‑4142707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑