Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,166,384	A→T	6.7%	P364P (CCT→CCA)	creA ←	creatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,166,384	0	A	T	6.7%	180.9 / 3.0	60	P364P (CCT→CCA)	creA	creatinase
Reads supporting (aligned to +/- strand):  ref base A (26/30);  new base T (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

CCCGCGAAGCAGCCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCCGAACGAATGGCCATACCCGAAGGTGCGGTACTG  >  NC_002947/4166239‑4166530                                                                                                                                                  |                                                                                                                                                    cccGCGAAGCAGCCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAgg                                                                                                                                                   <  5:287891/148‑1 (MQ=255)  ccGCGAAGCAGCCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCTgg                                                                                                                                                   >  8:198121/1‑147 (MQ=255)  ccGCGAAGCAGCCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAgg                                                                                                                                                   >  4:129378/1‑147 (MQ=255)  ccGCGAAGCAGCCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTAGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCTgg                                                                                                                                                   >  8:193088/1‑147 (MQ=255)        agcagcCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGTGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGa                                                                                                                                           <  8:223569/149‑1 (MQ=255)        agcagcCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGa                                                                                                                                           <  1:171253/149‑1 (MQ=255)            gcCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCa                                                                                                                                                     <  1:78303/135‑1 (MQ=255)            gcCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCa                                                                                                                                                     >  2:78303/1‑135 (MQ=255)            gcCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGatcat                                                                                                                                       >  6:124969/1‑149 (MQ=255)             ccGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGatcatc                                                                                                                                      <  3:268641/149‑1 (MQ=255)               gACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCgg                                                                                                                                    <  3:60364/149‑1 (MQ=255)                    cGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGgcag                                                                                                                                                >  4:114214/1‑132 (MQ=255)                    cGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGgcag                                                                                                                                                <  3:114214/132‑1 (MQ=255)                         cTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCtcg‑atcga                                                                                                                          <  4:137830/148‑1 (MQ=255)                         cTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGac                                                                                                                         <  5:124969/149‑1 (MQ=255)                         cTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGac                                                                                                                         <  3:235476/149‑1 (MQ=255)                                gCGAATGATGTTGTGCTCCGGCCCCTACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCgg                                                                                                                                    <  5:277009/132‑1 (MQ=255)                                gCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCgg                                                                                                                                    >  6:277009/1‑132 (MQ=255)                                gCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACAccacca                                                                                                                  >  2:107431/1‑149 (MQ=255)                                   aaTGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGc                                                                                                               >  6:329719/1‑149 (MQ=255)                                            gtgCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTcc                                                                                                       <  5:329719/148‑1 (MQ=255)                                            gtgCTACGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTAGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCGCG‑ATCGACACCACCATGCACGGTGccc                                                                                                      >  2:299432/1‑148 (MQ=255)                                             tgCTCCGGCCCATACGGGAACTTGGTGATGTTCTTGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCa                                                                                                      <  5:12662/148‑1 (MQ=255)                                             tgCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTTGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCa                                                                                                      <  1:107431/148‑1 (MQ=255)                                                  cGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGcaca                                                                                                 <  8:72414/148‑1 (MQ=255)                                                  cGGCCCATACGCGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCACGATGTCGTGCTCCCGATAACCGCCCG‑CCCCGGTCGCCCTTCAGGCAGCATCATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGcacac                                                                                                >  6:81258/1‑147 (MQ=255)                                                    gCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTa                                                                                              >  8:198700/1‑149 (MQ=255)                                                     cccATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTAt                                                                                             <  6:114294/149‑1 (MQ=255)                                                           cGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATg                                                                                        <  7:198700/148‑1 (MQ=255)                                                              gAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCt                                                                                    >  2:33793/1‑149 (MQ=255)                                                                  ttGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTcgcg                                                                                >  7:185847/1‑149 (MQ=255)                                                                        aTGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTc                                                                          >  3:62311/1‑149 (MQ=255)                                                                         tGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTcc                                                                         <  8:185847/149‑1 (MQ=255)                                                                             ctcGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGc                                                                      <  2:141838/148‑1 (MQ=255)                                                                                          tCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCTGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCgg                                                          <  7:193088/147‑1 (MQ=255)                                                                                          tCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCTGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCgg                                                          <  7:198121/147‑1 (MQ=255)                                                                                            gTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCg                                                       <  3:129378/148‑1 (MQ=255)                                                                                            gTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCg                                                       <  3:15464/148‑1 (MQ=255)                                                                                                    tCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATgg                                              >  3:132651/1‑149 (MQ=255)                                                                                                    tCAGGATGTCGTGCTCGCGATAACCGCACGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATgg                                              >  5:132053/1‑149 (MQ=255)                                                                                                       ggATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTg                                           >  7:255638/1‑149 (MQ=255)                                                                                                       ggATGTCGTGCTCGCGATAACCGCCCG‑CCCCGGTCGCGCTTCAGGGAGCATGATCATCGGCTCG‑ATCGACACCTCGATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGGCCCGCCTCGCGGCCGTAGTAATGGCTGa                                          <  5:81258/149‑1 (MQ=255)                                                                                                        gATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCt                                            >  5:303604/1‑147 (MQ=255)                                                                                                              gTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGc                                    >  2:12618/1‑149 (MQ=255)                                                                                                               tGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGcaca                                                                                                 >  3:39216/1‑87 (MQ=255)                                                                                                               tGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGcaca                                                                                                 <  4:39216/87‑1 (MQ=255)                                                                                                               tGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGcc                                   >  8:28930/1‑149 (MQ=255)                                                                                                                  tCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCcgaa                                >  3:66449/1‑149 (MQ=255)                                                                                                                    gcgATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGacac                                                                                                                       >  7:294076/1‑60 (MQ=255)                                                                                                                    gcgATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGacac                                                                                                                       <  8:294076/60‑1 (MQ=255)                                                                                                                      gATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCcgaacgag                            >  5:251931/1‑148 (MQ=255)                                                                                                                      gATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCcgaac                               <  6:251931/146‑1 (MQ=255)                                                                                                                      gATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGAAACCAGCACAGTATCGATGTACTCGCGCAGCTCCAGCCCCCCCTCGCGGCCGTAGTAATGGCTGAGCGTGCcgaacgaa                            >  1:261168/1‑149 (MQ=255)                                                                                                                       aTAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGc                                        >  4:129657/1‑136 (MQ=255)                                                                                                                       aTAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGc                                        <  3:129657/136‑1 (MQ=255)                                                                                                                          aCCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCGAATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCCGAACGAATgg                         >  1:71678/1‑149 (MQ=255)                                                                                                                          aCCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCCGAACGAATGGc                        <  1:33793/149‑1 (MQ=255)                                                                                                                          aCCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCCGAACGAATGGc                        <  8:255638/149‑1 (MQ=255)                                                                                                                           ccgcccgcCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCCGAACGAATGGcc                       <  7:28930/149‑1 (MQ=255)                                                                                                                                     ccGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCCGAACGAATGGCCATACCCGAAg             <  4:62311/149‑1 (MQ=255)                                                                                                                                           gCCCTTCAGGCAGCATGATCCTCGGCTCG‑ATCGACCCCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCTCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCCGAACGAATGGCCATACCCGAAGGTGCg        <  1:299432/148‑1 (MQ=255)                                                                                                                                                tCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGACGAACGAATGGCCATACCCGAAGGTGCGGTACTg  >  7:200118/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CCCGCGAAGCAGCCGACGCCGATGCTTATTTGCGAATGATGTTGTGCTCCGGCCCATACGGGAACTTGGTGATGTTCTCGGCGCCGCTGTCGTTGACGATCAGGATGTCGTGCTCGCGATAACCGCCCGCCCCCGGTCGCCCTTCAGGCAGCATGATCATCGGCTCG‑ATCGACACCACCATGCCCGGTTCCAGCACAGTATCGATGTCCTCGCGCAGCTCCAGCCCCGCCTCGCGGCCGTAGTAATGGCTGAGCGTGCCGAACGAATGGCCATACCCGAAGGTGCGGTACTG  >  NC_002947/4166239‑4166530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑