Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,190,249 C→G 8.3% P140P (CCG→CCC)  PP_5612 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,190,2490CG 8.3% 135.4 / 5.3 48P140P (CCG→CCC) PP_5612hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (19/25);  new base G (2/2);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

CTCCGCCTTCTGCCACTCTGAGATGAACCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACTTTAGAATTTTTTTAGCCCGACGATCCCCAGGTACGAGTGG  >  NC_002947/4190102‑4190386
                                                                                                                                                   |                                                                                                                                         
cTCCGCCTTCTGCCACTCTGAGATGAACCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCg                                                                                                                                          >  6:50970/1‑149 (MQ=255)
              aCTCTGAGATGAACCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGAt                                                                                                                                       <  7:235230/138‑1 (MQ=255)
              aCTCTGAGATGAACCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGAt                                                                                                                                       >  8:235230/1‑138 (MQ=255)
                tctGAGATGAACCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCagaag                                                                                                                           >  7:51751/1‑148 (MQ=255)
                  tGAGATGAACCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGaaa                                                                                                                        >  1:88004/1‑149 (MQ=255)
                       tGAACCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCaa                                                                                                                    <  8:51751/148‑1 (MQ=255)
                         aaCCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCGAATGGGATAccc                                                                                                                                   <  4:41374/131‑3 (MQ=255)
                         aaCCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCGAATGGGATAccc                                                                                                                                   <  4:37338/131‑3 (MQ=255)
                         aaCCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCGAATGGGATAccc                                                                                                                                   >  3:41374/1‑129 (MQ=255)
                         aaCCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCGAATGGGATAccc                                                                                                                                   >  3:37338/1‑129 (MQ=255)
                            ctaaTCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCTATTCTCGGTTAAGGCAAAGGAAACGCTCGCGCCACGGCTGCTGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGa                                                                                                              <  1:215839/146‑1 (MQ=255)
                               aTCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGaaa                                                                                                            <  3:219242/148‑1 (MQ=255)
                               aTCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGaaa                                                                                                            <  2:115715/148‑1 (MQ=255)
                                  cAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCt                                                                                                         <  1:275674/148‑1 (MQ=255)
                                         gATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGa                                                                                                              <  8:223368/136‑1 (MQ=255)
                                         gATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGa                                                                                                              >  7:223368/1‑136 (MQ=255)
                                            aataCGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGAt                                                                                                                                       <  7:248698/108‑1 (MQ=255)
                                            aataCGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGAt                                                                                                                                       >  8:248698/1‑108 (MQ=255)
                                                       tctcGTTGCCGGATCGAGTCTCGGTTAATGCACAGGAAACGGTCGCGCCACGGCTGGGGGTTTTGTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAAc                                                                                                           <  6:153641/125‑1 (MQ=255)
                                                       tctcGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACACAGAAGAAACCAAGTTCGAAAc                                                                                                           >  5:153641/1‑125 (MQ=255)
                                                               ccGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATa                                                                            >  3:72052/1‑148 (MQ=255)
                                                               ccGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATAc                                                                           <  4:72052/149‑1 (MQ=255)
                                                               ccGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACATGATCAGCACTGACCATTTCCTGATTAATa                                                                            >  7:63542/1‑148 (MQ=255)
                                                                      gAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTc                                                                    <  2:88004/149‑1 (MQ=255)
                                                                       aGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTcc                                                                   <  5:50970/149‑1 (MQ=255)
                                                                         tctcGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGaa                                                                                                             <  3:33090/105‑1 (MQ=255)
                                                                         tctcGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGaa                                                                                                             >  4:33090/1‑105 (MQ=255)
                                                                                  aGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAg                                                        <  7:282524/149‑1 (MQ=255)
                                                                                   ggCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAAc                                                                                                           <  8:24735/97‑1 (MQ=255)
                                                                                   ggCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAAc                                                                                                           >  7:24735/1‑97 (MQ=255)
                                                                                     cacaGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACa                                                                          >  6:112789/1‑128 (MQ=255)
                                                                                     cacaGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACa                                                                          <  5:112789/128‑1 (MQ=255)
                                                                                     cacaGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAggtg                                                     <  3:30554/149‑1 (MQ=255)
                                                                                      acaGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAggtgg                                                    >  8:249014/1‑149 (MQ=255)
                                                                                        aGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAGGTGGTc                                                  <  5:252888/149‑1 (MQ=255)
                                                                                                           gCTGCGTGATTTCTTCACATCGCACAAATGGTAGCCCTTTCGGATTCGACTCAGTATAAACCAAGTTCGTAACCTGATCAGCACTGAGCATTTGCTGATAAATAGAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACTTTAGAAttttt                               <  8:63542/149‑1 (MQ=255)
                                                                                                             tGCGGGATTTCTTCACATCGCACAAAGGGTATCCCAATCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGATCCTCGTAGTCAAGGTGCTCGAGCCACTTTAGAAtttttt                              >  4:247583/1‑148 (MQ=255)
                                                                                                                gggATTTCTTCACATCGCACAAAGGTTATCCGATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTTATTATAAATACAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACTTTAGAATTTTTTTAGc                          <  5:140500/149‑1 (MQ=255)
                                                                                                                        ttcACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACttt                                        <  1:78004/127‑1 (MQ=255)
                                                                                                                        ttcACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACttt                                        >  2:78004/1‑127 (MQ=255)
                                                                                                                               cGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACTTTAGAATTTTTTTAGCCCGACGATCCCCAgg           >  5:54658/1‑149 (MQ=255)
                                                                                                                                 cacaAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGtt                                                                     >  7:131140/1‑89 (MQ=255)
                                                                                                                                 cacaAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGtt                                                                     <  8:131140/89‑1 (MQ=255)
                                                                                                                                       gggTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACTTTAGAATTTTTTTAGCCCGACGATCCCCAGGTACGAgtg   <  7:249014/149‑1 (MQ=255)
                                                                                                                                          tATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACTTTAGAATTTTTTTAGCCCGACGATCCCCAGGTACGAgtgg  >  8:118212/1‑147 (MQ=255)
                                                                                                                                          tATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACTTTAGAATTTTTTTAGCCCGACGATCCCCAGGTACGAgtgg  <  7:118212/147‑1 (MQ=255)
                                                                                                                                                aTTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCg                                                                       >  3:114870/1‑72 (MQ=255)
                                                                                                                                                aTTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCg                                                                       <  4:114870/72‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
CTCCGCCTTCTGCCACTCTGAGATGAACCTCATCCAGTCATGATAATACGCGACCTCTCGTTCCCGGATCGAGTCTCGGTTAAGGCACAGGAAACGCTCGCGCCACGGCTGCGGGATTTCTTCACATCGCACAAAGGGTATCCCATTCGGATACGACTCAGAAGAAACCAAGTTCGAAACCTGATCAGCACTGAGCATTTGCTGATAAATACAGCGTTCCTCGTAGTCAAGGTGGTCGAGCCACTTTAGAATTTTTTTAGCCCGACGATCCCCAGGTACGAGTGG  >  NC_002947/4190102‑4190386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑