Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,233,685 G→T 9.0% H460Q (CAC→CAA)  PP_3711 ← diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,233,6850GT 9.0% 163.2 / 2.9 53H460Q (CAC→CAA) PP_3711diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (22/26);  new base T (3/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAGGGGTATGTGTCTAGAAAATGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAGCCCAGCAGTTGCTCTGC  >  NC_002947/4233538‑4233831
                                                                                                                                                    |                                                                                                                                                  
tGAAGGGGTATGTGTCTAGAAAATGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGt                                                                                                                                                   >  5:271562/1‑149 (MQ=255)
        tATGTGTCTAGAAAATGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCt                                                                                                                                           <  8:126745/149‑1 (MQ=255)
           gtgtCTAGAAAATGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGGCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCtgt                                                                                                                                         <  1:258986/148‑1 (MQ=255)
            tgtCTAGAAAATGAAGTCCATCGGCATGACCATGCCACGGGTCGGGGGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAGCGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCtgtg                                                                                                                                        <  6:270353/148‑1 (MQ=255)
                tAGAAAATGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTg                                                                                                                                                  >  6:243303/1‑134 (MQ=255)
                tAGAAAATGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTg                                                                                                                                                  <  5:243303/134‑1 (MQ=255)
                tAGAAAATGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAg                                                                                                                                   >  1:152681/1‑149 (MQ=255)
                    aaaTGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCg                                                                                                                               <  2:200098/149‑1 (MQ=255)
                    aaaTGAAGTCCATCGGCATGAACATGCCCCGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCAGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGTTGCAGCGCGGTGCGCAGGTCg                                                                                                                               >  3:81106/1‑149 (MQ=255)
                     aaTGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCACCTG‑GGGCAGTTGCAgtt                                                                                                                                             <  5:136688/134‑3 (MQ=255)
                     aaTGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCACCTG‑GGGCAGTTGCAgtt                                                                                                                                             >  6:136688/1‑132 (MQ=255)
                          aGTCCATCGGCAGGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTc                                                                                                                          <  7:234815/148‑1 (MQ=255)
                              cATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTccac                                                                                                                       >  7:171971/1‑147 (MQ=255)
                                           aTGCGACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACTGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTggcg                                                                                                        <  4:132214/149‑1 (MQ=255)
                                              ccACGGGGCGGGGGTTCCCAGCTCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTggcgggc                                                                                                     <  6:225210/149‑1 (MQ=255)
                                                       gtgtgtTACCAGCTCAGCAGATCCTCATAACGTGTAACGATGCTGGTCGGATGATCGAATACCTGCTCGTACTGCATCAGCAACTG‑CCCCAGGTGCAGCGCTGTGCTCAGGACGCTTTCCACCCTGCGGCGCGGGCGGGCGGCACCGTc                                                                                            >  8:235352/3‑149 (MQ=255)
                                                       gggtGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTc                                                                                            <  8:36734/149‑1 (MQ=255)
                                                       gaatgatCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTc                                                                                            <  8:277139/143‑1 (MQ=255)
                                                            ttCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTTCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGtt                                                                                       <  3:151512/149‑1 (MQ=255)
                                                            ttCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGtt                                                                                       <  3:156260/149‑1 (MQ=255)
                                                              cccAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCACCTG‑GGGCAGTTGCAgt                                                                                                                                              <  2:299403/92‑2 (MQ=255)
                                                              cccAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCACCTG‑GGGCAGTTGCAgt                                                                                                                                              >  1:299403/1‑91 (MQ=255)
                                                              cccAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTc                                                                                      <  1:318467/148‑1 (MQ=255)
                                                                      agcagcGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTGCCCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGgcggcg                                                                                                              <  1:62440/117‑1 (MQ=255)
                                                                      agcagcGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGgcggcg                                                                                                              >  2:62440/1‑116 (MQ=255)
                                                                              cTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCAt                                                                     <  6:271562/149‑1 (MQ=255)
                                                                                    aCCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGAt                                                               >  2:193158/1‑149 (MQ=255)
                                                                                             gctgctGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGt                                                                                        <  8:86120/115‑1 (MQ=255)
                                                                                             gctgctGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGt                                                                                        >  7:86120/1‑115 (MQ=255)
                                                                                             gctgctGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGcc                                                        >  2:274604/1‑147 (MQ=255)
                                                                                             gctgctGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGcc                                                        <  1:274604/147‑1 (MQ=255)
                                                                                                    gTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCtt                                               <  8:171971/149‑1 (MQ=255)
                                                                                                     tCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCTGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTg                                              <  2:152681/149‑1 (MQ=255)
                                                                                                        gatgatCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGcc                                           >  7:293023/1‑149 (MQ=255)
                                                                                                         atgatCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTgg                                             <  7:235352/146‑1 (MQ=255)
                                                                                                                     cGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTAAGGCCGTTACGCTTGGCCTGGTACAGCGCCa                              >  7:178532/1‑149 (MQ=255)
                                                                                                                            tAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGc                       >  7:26484/1‑149 (MQ=255)
                                                                                                                               tgcaactgcaacTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCg                      >  6:136473/1‑147 (MQ=255)
                                                                                                                                gcaactgcaactG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACGTCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAg                   >  3:236500/1‑149 (MQ=255)
                                                                                                                                gcaactgcaactG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAg                   >  4:111962/1‑149 (MQ=255)
                                                                                                                                gcaactgcaactG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAg                   >  4:3454/1‑149 (MQ=255)
                                                                                                                                        aactg‑cCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTgg                                        <  2:71655/120‑1 (MQ=255)
                                                                                                                                        aactg‑cCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTgg                                        >  1:71655/1‑120 (MQ=255)
                                                                                                                                           tg‑cCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAGCCCAGCAGTTg        <  5:183489/149‑1 (MQ=255)
                                                                                                                                              ccccAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAGCCcagcag           >  3:27248/1‑144 (MQ=255)
                                                                                                                                              ccccAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAGCCcagcag           <  4:27248/144‑1 (MQ=255)
                                                                                                                                              ccccAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAGCCCAGCAGTTGct      >  7:263257/1‑149 (MQ=255)
                                                                                                                                               cccAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAGCCCAGCAGTTGctc     <  3:111962/149‑1 (MQ=255)
                                                                                                                                                ccAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGt                     >  1:306096/1‑132 (MQ=255)
                                                                                                                                                ccAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGt                     <  2:306096/132‑1 (MQ=255)
                                                                                                                                                ccAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAGCCCAGCAGTTGctcg    >  8:261004/1‑148 (MQ=255)
                                                                                                                                                ccAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCATGGTACAGCGCCATGTCAGCGTAGCCCAGAAGTTGctct    >  6:85189/1‑149 (MQ=255)
                                                                                                                                                  aGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAGCCCAGCAGTTGCTCTGc  <  8:178532/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGAAGGGGTATGTGTCTAGAAAATGAAGTCCATCGGCATGACCATGCCACGGGTCGGGTGTTCCCAGCGCAGCAGCGCCTCATAACCGGTAACGCTGCTGGTCTGATGATCGACTACCGGCTGGTAGTGCAACTGCAACTG‑CCCCAGGTGCAGCGCTGTGCGCAGGTCGGTTTCCACCAGGCGGCGCTGGCGGGCGGCAACGTCCAGTTCGACATCGAAGCATTCATAACGATTACGGCCGTTACGCTTGGCCTGGTACAGCGCCATGTCAGCGTAGCCCAGCAGTTGCTCTGC  >  NC_002947/4233538‑4233831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑