Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947195,6340CT37.5% 29.0 / 35.0 44T380T (ACC→ACT) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (12/13);  new base T (8/7);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAG  >  NC_002947/195499‑195778
                                                                                                                                       |                                                                                                                                                
cccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTg                                                                                                                                      <  2:24317/148‑1 (MQ=39)
     cgaaTGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGc                                                                                                                                 <  1:75016/146‑1 (MQ=2)
               cTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCg                                                                                                                       <  3:101488/148‑1 (MQ=37)
                     caacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAc                                                                                                                  >  6:114746/1‑147 (MQ=37)
                                ccGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                                                                                                      <  5:114746/148‑1 (MQ=37)
                                ccGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                                                                                                      <  3:10828/148‑1 (MQ=17)
                                 cGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTg                                                                                                     <  8:78711/148‑1 (MQ=38)
                                    catcGTCACCATCTACAACGCCACCGGCGGTAATTTCGAGCAACTGTCACAGAATCCCACCCCCGCTCCGACCTCGCTGATCGACTCTTTCGACACCACAACCGCGACCCTGACGGCGAGCCCTTCGGTCACCGAAGGCGGCGTGATc                                                                                                  <  8:24627/145‑1 (MQ=11)
                                       cGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGgcggcg                                                                                                       >  6:102234/1‑140 (MQ=17)
                                        gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                                                                                                      <  5:102234/140‑1 (MQ=17)
                                         tCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACGACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTa                                                                                             >  1:91300/1‑148 (MQ=12)
                                           aCCATCGAGAACCCCACAGGCGGTAATTTCGAGCAACTGCCCCCGACTCCGACCCCGCCACAGACCACGATCAACGACTCGGTCGACCCGACTACCGTGCCCCTCACGGCGAGCCCGTCCGGCCCCGAAGGCGGCGAGAACACTTaca                                                                                           >  8:110062/1‑148 (MQ=11)
                                             cATCGAGAACGCCACCGGCGGTAATTTCGAGCATCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACAcc                                                                                         >  7:75350/1‑148 (MQ=21)
                                             cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACAACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGa                                                                                                               >  3:23280/1‑126 (MQ=17)
                                              aTCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGaa                                                                                                              <  4:23280/126‑1 (MQ=17)
                                                 gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGCTCAACGACTCGGTCGACACCACTACCGCGACCCTGCCGGCGAGCCCGTCGGTCACCGAAGgcggcg                                                                                                       >  5:89372/1‑130 (MQ=255)
                                                  agaACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGa                                                                                     <  2:169410/147‑1 (MQ=39)
                                                  agaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGt                                                                                                      <  6:89372/130‑1 (MQ=17)
                                                    aaCGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTac                                                                                            >  7:69370/1‑138 (MQ=21)
                                                     aCGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTaca                                                                                           <  8:69370/138‑1 (MQ=21)
                                                            cggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCg                                                                                                                                >  1:60796/1‑94 (MQ=38)
                                                             ggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGa                                                                                                                               <  2:60796/94‑1 (MQ=37)
                                                              gcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                                      >  2:102992/1‑134 (MQ=37)
                                                               cggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGa                                                                                     <  1:102992/134‑1 (MQ=37)
                                                                     tttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCa                                                                  <  1:163407/147‑1 (MQ=17)
                                                                     tttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACAACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACATACACCGTGACCCTGAGCAATCCTGCCCAg                                                                 >  6:16158/1‑148 (MQ=12)
                                                                      ttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACGGTGACCCTGAGCAACCCTGCCCAGa                                                                >  5:159306/1‑148 (MQ=255)
                                                                         gAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCCCCCCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGCCCCTGAGCCACCCTGCCCAGACGc                                                             >  5:43744/1‑148 (MQ=11)
                                                                               cTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACATACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTaa                                                       <  5:16158/148‑1 (MQ=9)
                                                                                    cccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGCCGAGCCCGTCGGTCACCGAAGGCGCCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGt                                                   >  5:132586/1‑147 (MQ=255)
                                                                                     ccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGa                                                 >  2:135313/1‑148 (MQ=255)
                                                                                      cGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGac                                                >  1:60150/1‑147 (MQ=9)
                                                                                                     cTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGcc                                  >  3:15748/1‑147 (MQ=39)
                                                                                                          aCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGa                            >  3:96808/1‑148 (MQ=38)
                                                                                                            cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGAtt                          >  3:82645/1‑148 (MQ=255)
                                                                                                             aCGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACATACACCGTGACCCTGAGCAAACCTGCCCAGACGCCGGTAACAGTGAACCTGTCCAACGGCCAAACGATTa                         >  6:57841/1‑148 (MQ=12)
                                                                                                              cGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTAc                        <  2:51207/148‑1 (MQ=255)
                                                                                                               gATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTAcc                       <  5:66828/148‑1 (MQ=255)
                                                                                                                 tCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACCCCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGt                     >  7:33313/1‑148 (MQ=255)
                                                                                                                   aaCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGtt                    >  3:15242/1‑147 (MQ=255)
                                                                                                                        cTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCg              >  2:73627/1‑148 (MQ=255)
                                                                                                                          cGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGt                                                   <  7:34779/109‑1 (MQ=12)
                                                                                                                               gACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTaaaa       <  1:135313/148‑1 (MQ=39)
                                                                                                                                acacCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACTGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACTGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTaaaa       <  6:159306/147‑1 (MQ=21)
                                                                                                                                    cactaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg  >  2:146229/1‑148 (MQ=255)
                                                                                                                                    caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACGTACACCGTGAGCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGacc                                               >  3:10138/1‑101 (MQ=11)
                                                                                                                                     accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGAccc                                              <  4:10138/103‑1 (MQ=12)
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CCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAG  >  NC_002947/195499‑195778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑