Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947197,5720GA26.7% 71.4 / 25.9 46K1026K (AAG→AAA) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (18/15);  new base A (5/7);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACC  >  NC_002947/197431‑197716
                                                                                                                                             |                                                                                                                                                
caccacCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAg                                                                                                                                            >  5:139531/1‑148 (MQ=37)
 accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAgg                                                                                                                                           >  1:74407/1‑148 (MQ=37)
 accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAgg                                                                                                                                           >  2:25709/1‑148 (MQ=37)
      cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGc                                                                                                                                      >  2:138643/1‑148 (MQ=37)
            ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAACCCATTACCGGTGAAGCCGGCAAGACCCAGGGCAGCGTCGAt                                                                                                                                >  6:32523/1‑148 (MQ=255)
             cTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAtt                                                                                                                               >  5:94717/1‑148 (MQ=37)
                 cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTc                                                                                                                             <  1:25709/146‑1 (MQ=37)
                                 cACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc                                                                                                           <  5:84332/148‑1 (MQ=37)
                                 cACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc                                                                                                           <  6:131563/148‑1 (MQ=37)
                                   ccGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCGGCGTCGATTTCCAGACCCCGGCGAATGACGt                                                                                                         >  7:28751/1‑148 (MQ=37)
                                      aaGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTa                                                                                                      <  8:18203/148‑1 (MQ=37)
                                                gATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGt                                                                                            >  8:144564/1‑148 (MQ=37)
                                                gATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGt                                                                                            >  7:119206/1‑148 (MQ=37)
                                                gATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGt                                                                                            >  1:35768/1‑148 (MQ=37)
                                                 aTCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGtt                                                                                           >  4:90599/1‑148 (MQ=37)
                                                  tCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCGGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTc                                                                                          <  8:28751/148‑1 (MQ=37)
                                                      cTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACt                                                                                      >  2:70498/1‑148 (MQ=38)
                                                      cTACACCGTGAGCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCACACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGAcc                                                                                      <  5:121021/148‑2 (MQ=18)
                                                                 tCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGCCCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACAGTCAGCGTCa                                                                            >  4:15956/1‑147 (MQ=25)
                                                                     gAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAAc                                                                                                                                                <  8:73321/75‑1 (MQ=255)
                                                                     gAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAGTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCAt                                                                        >  5:133282/1‑147 (MQ=37)
                                                                            ccTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAGTTCCAGACCCCGGCGAATGACGTCTa                                                                                                      >  3:51751/1‑110 (MQ=25)
                                                                             cTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAACACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTac                                                                                                     <  4:51751/110‑1 (MQ=255)
                                                                                cccAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGGCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCcac                                                            <  6:94717/148‑1 (MQ=17)
                                                                                 ccAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCca                                                             <  2:74407/146‑1 (MQ=17)
                                                                                         cGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTaa                                                   <  6:133282/148‑1 (MQ=37)
                                                                                         cGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTaa                                                   <  1:138643/148‑1 (MQ=37)
                                                                                          ggTAACCGTGACCCTGTCCAAACGCCAAACCATTCCCGATGCATACCGCAAGACCCAGGGCAGAGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCTTCGAGAACGGCCCAGGCGGGAAt                                                  >  4:101325/1‑148 (MQ=11)
                                                                                           gTAACCGTGACCCTGACCAACGGACAAACCATTACCGTAGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGCCCCCGGCGAATGACGTCTACAACAACGCAACGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAAtt                                                 >  8:98362/1‑148 (MQ=11)
                                                                                            tAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAAttt                                                <  6:76259/148‑1 (MQ=35)
                                                                                               ccGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGt                                                     >  1:9892/1‑140 (MQ=255)
                                                                                                cGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTa                                                    <  2:9892/140‑1 (MQ=16)
                                                                                                cGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAg                                            <  6:43744/148‑1 (MQ=12)
                                                                                                   gACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCaa                                         >  3:38460/1‑148 (MQ=32)
                                                                                                     cccTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACt                                       <  5:151661/148‑1 (MQ=12)
                                                                                                     cccTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAAc                                        <  1:70498/147‑1 (MQ=32)
                                                                                                       cTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGa                                     <  8:107608/148‑1 (MQ=12)
                                                                                                                 gCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGAGTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAAccc                           <  3:15956/148‑1 (MQ=21)
                                                                                                                           tACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACACCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGc                  >  7:11179/1‑147 (MQ=255)
                                                                                                                               gTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGa             <  4:18027/148‑1 (MQ=21)
                                                                                                                                  gAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGCTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGCTAATTTCGAGCAACTGACCCCGACCCCGACCCCGGCTCAGCCCa          >  1:71754/1‑148 (MQ=11)
                                                                                                                                      ccGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCg                                              >  5:72461/1‑108 (MQ=12)
                                                                                                                                      ccGGCAAAACCCAGGGCAGCGTCGACTTCCAGACACCGGCGAATCACGTCTACAACAACGGTTCGACTGTCAGCGTAACCCTCGAGAACGCCAAAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGa       >  4:146187/1‑147 (MQ=11)
                                                                                                                                       cGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa                                             <  6:72461/108‑1 (MQ=12)
                                                                                                                                          cAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAcc  <  3:90599/148‑1 (MQ=38)
                                                                                                                                          cAAGACCCAGCGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTGCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCATACCACGATCAcc  <  8:119206/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
CACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACC  >  NC_002947/197431‑197716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑