Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,403,5910TC32.1% 26.5 / 20.4 33intergenic (‑57/+24)PP_1227/PP_1228membrane protein/methyl‑accepting chemotaxis transducer
Reads supporting (aligned to +/- strand):  ref base T (10/9);  new base C (7/2);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.32e-03
Rejected as consensus: Frequency below/above cutoff threshold.

GATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTC  >  NC_002947/1403448‑1403727
                                                                                                                                               |                                                                                                                                        
gATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGccc                                                                                                                                      >  2:143293/1‑148 (MQ=255)
                gcgagcgaGGTGCGTAAAAGCCCTTGTTCGCTGGTGAGGTCGAAGAACGTGGAGCGGGTGGGATTCGGCATAAGTTGTGCGCAAGACTAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGAc                                                                                                                      <  3:57493/148‑1 (MQ=38)
                gcgATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGAaga                                                                                                                               >  4:37496/1‑139 (MQ=255)
                 cgATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGGTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCACGCCCGCGAagag                                                                                                                              <  3:37496/139‑1 (MQ=255)
                              tAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGaa                                                                                                        >  1:10879/1‑148 (MQ=255)
                               aaaaGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGaaa                                                                                                       >  4:21572/1‑148 (MQ=255)
                                      cTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCACTACGCGGGCGGGCCCGCGCAGAGGCCCGGACAGGCTAGACGCGCAACGGcag                                                                                                 >  6:21349/1‑145 (MQ=255)
                                                  tGATGTCGAAGAAAGGGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCct                                                                                      <  6:110742/146‑1 (MQ=255)
                                                       tCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCctgctgctg                                                                               >  6:46491/1‑148 (MQ=255)
                                                         gaagaaAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGt                                                                             >  7:147131/1‑148 (MQ=255)
                                                            gaaAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTgg                                                                          >  2:16415/1‑148 (MQ=255)
                                                                 gggATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGc                                                                     <  6:12670/147‑1 (MQ=255)
                                                                        tgTTGGATTAGGCATAAGTTGGGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCa                                                              <  8:149009/147‑1 (MQ=255)
                                                                        ggTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGGGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCa                                                              <  2:47575/148‑1 (MQ=255)
                                                                           tGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCctgctgctg                                                                               >  8:23053/1‑128 (MQ=255)
                                                                            ggATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGGGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTgg                                                                              <  7:23053/128‑1 (MQ=255)
                                                                              aTTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCtt                                                        <  6:80407/148‑1 (MQ=255)
                                                                                  gccatccgTTGTGCGCAAGAATACCGATGGGGCGGGCCCGGCCCGGCCCGCTTCGCGGCCGGGCCCGCGATGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGc                                                     <  5:21349/140‑1 (MQ=255)
                                                                                         gTTGTGCTCAATAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGt                                             <  2:79971/148‑1 (MQ=255)
                                                                                              gCTCAATTATAAAGATGGTGCGGTGCCTGTCAGGGCCTCTTCGGGTGCGTGCCCGCGATGAGTCCCTGAGAGGCTAGACGCGAAAGTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGa                                        <  8:76007/148‑1 (MQ=255)
                                                                                              gCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGCCAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATTCGTGCCGa                                        >  1:54301/1‑148 (MQ=255)
                                                                                                tCAAGAATACCGATGGTGCGGGGCCTGGCAGGGCCTCTTCGCGGGCGGGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGAt                                       <  3:29229/147‑1 (MQ=255)
                                                                                                     aaTACCGAGGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTTATGCGTGCCGATTCATCg                                 <  3:30401/148‑1 (MQ=255)
                                                                                                                            cAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCCGCCCAGCCAGTGATTCTGt          <  3:120627/148‑1 (MQ=255)
                                                                                                                               ggCCTCTTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGtt                                                                            >  3:125659/1‑79 (MQ=255)
                                                                                                                               ggCCTCTTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGGTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCAc       >  3:8144/1‑148 (MQ=255)
                                                                                                                                gCCTCTTCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTg                                                                           <  4:125659/79‑1 (MQ=255)
                                                                                                                                  ctctTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGACGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGtc    >  1:58525/1‑148 (MQ=255)
                                                                                                                                   tctTCGCGGGCACGCCCGCGAAGAGGCCCTGCCAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGGTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGtct   >  1:2848/1‑148 (MQ=255)
                                                                                                                                     ttCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGGCAGTGATTCTGTCACGtctc  >  7:19468/1‑147 (MQ=255)
                                                                                                                                     ttCGCGGGCACGCCCGCGAAGAGGCCCTGACCGGCTAGCCGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGtctc  >  3:120187/1‑147 (MQ=255)
                                                                                                                                     ttCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGtctc  >  5:48896/1‑147 (MQ=255)
                                                                                                                                     ttCGCGGGCACGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGtctc  >  3:47142/1‑147 (MQ=255)
                                                                                                                                               |                                                                                                                                        
GATGAACAGGGTGGCGGCGATCGAGGTGCGTAAAAGCCCTTGTTCGCTGGTGATGTCGAAGAAAGTGGATCGGGTTGGATTAGGCATAAGTTGTGCTCAAGAATAACGATGGTGCGGTGCCTGTCAGGGCCTCTTCGCGGGCGTGCCCGCGAAGAGGCCCTGACAGGCTAGACGCGAAACTGCTCCATCAGCGCCTGCTGCTGGTTGGCCAGCCGGTTCAGGGCTTGGCTGATGCGTGCCGATTCATCGGCCTGCCCAGCCAGTGATTCTGTCACGTCTC  >  NC_002947/1403448‑1403727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑