Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,804,313	A→G	E42E (GAA→GAG)	rbsR →	DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,804,313	0	A	G	100.0%	79.4 / NA	26	E42E (GAA→GAG)	rbsR	DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

CGGCGGAACTGACAACATGGCAACCATCAAAGACGTCGCGGCACTGGCGGGTATTTCCTACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACG  >  NC_002947/2804172‑2804452                                                                                                                                              |                                                                                                                                            cggcggAACTGACAACATGGCAACCATCAAAGACGTCGCGGCACTGGCGGGTATTTCCTACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGAt                                                                                                                                       <  4:83754/148‑1 (MQ=255)             caacaTGGCAACCATCAAAGACGTCGCGGCACTGGCGGGTATTTCCTACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGt                                                                                                                           <  6:68199/148‑1 (MQ=255)                caTGGCAACCATCAAAGACGTCGCGGCACTGGCGGGTATTTCCTACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCg                                                                                                                        >  3:154919/1‑148 (MQ=255)                 aTGGCAACCATCAAAGACGTCGCGGCACTGGCGGGTATTTCCTACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCgg                                                                                                                       >  5:133810/1‑148 (MQ=255)                     cAACCATCAAAGACGTCGCGGCACTGGCGGGTATTTCCTACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGgc                                                                                                                   <  6:40842/148‑1 (MQ=255)                          aTCAAAGACGTCGCGGCACTGGCGGGTATTTCCTACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCTcgct                                                                                                              >  8:32166/1‑148 (MQ=255)                                      gcgGCACTGGCGGGTATTTCCTACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAgcgc                                                                                                  >  2:106939/1‑148 (MQ=255)                                                           tACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGcc                                                                             >  2:129041/1‑148 (MQ=255)                                                            acacCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCt                                                                            >  1:8867/1‑148 (MQ=255)                                                                caccGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTg                                                                        <  3:74581/148‑1 (MQ=255)                                                                   cGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTg                                                                     >  3:78011/1‑148 (MQ=255)                                                                          cATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACa                                                              >  1:57872/1‑148 (MQ=255)                                                                                cTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCa                                                        >  5:60528/1‑148 (MQ=255)                                                                                 tCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCaa                                                       >  2:113792/1‑148 (MQ=255)                                                                                    acaaGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAAc                                                      <  2:110478/146‑1 (MQ=255)                                                                                           ccGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGcc                                                                   >  5:48618/1‑126 (MQ=255)                                                                                           ccGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTc                                             <  4:154919/148‑1 (MQ=255)                                                                                            cGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTCCGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCg                                            <  7:75662/148‑1 (MQ=255)                                                                                            cGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGccc                                                                  <  6:48618/126‑1 (MQ=255)                                                                                               ccGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCg                                         <  7:32166/148‑1 (MQ=255)                                                                                                            aGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCgggg                            <  5:77071/148‑1 (MQ=255)                                                                                                                   gCTGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGaa                     <  8:87326/148‑1 (MQ=255)                                                                                                                     tGAAAGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGa                   >  8:93065/1‑148 (MQ=255)                                                                                                                        aaGTGGAAGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGc                <  8:80884/148‑1 (MQ=255)                                                                                                                              aaGCCGCCATCATCGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGa          <  5:15154/148‑1 (MQ=255)                                                                                                                                      atcatcGAGCTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACg  <  5:25944/148‑1 (MQ=255)                                                                                                                                              |                                                                                                                                            CGGCGGAACTGACAACATGGCAACCATCAAAGACGTCGCGGCACTGGCGGGTATTTCCTACACCACCGTGTCCCATGTACTCAACAAGACCCGTCCGGTCAGCGAGCAGGTGCGGCTGAAAGTGGAAGCCGCCATCATCGAACTGGATTACGTGCCCAGTGCGGTGGCGCGCTCGCTGAAAGCGCGCAGCACGGCCACCATCGGCCTGTTGGTGCCCAACAGCGTCAACCCGTACTTCGCCGAGCTGGCGCGGGGTATCGAAGACGCCTGCGAGCGCAACG  >  NC_002947/2804172‑2804452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑