Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,947,557	G→C	P27R (CCG→CGG)	fliQ ←	flagellar export apparatus protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,947,557	0	G	C	100.0%	97.6 / NA	30	P27R (CCG→CGG)	fliQ	flagellar export apparatus protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (19/11);  total (19/11)

TCCATGAACTTCTGCACCAGCCACGGCCCGGCGATGATCAGCGTGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCGGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGACGCCGCCGAAACTGCTGGCCAGGGTGCCCATGA  >  NC_002947/4947413‑4947695                                                                                                                                                 |                                                                                                                                           tCCATGAACTTCTGCACCAGCCACGGCCCGGCGATGATCAGCGTGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGca                                                                                                                                         >  7:83571/1‑148 (MQ=255)        aCTTCTGCACTAGCCACGGCCCGGCGATGATCAGCGTGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAg                                                                                                                                  >  3:97171/1‑148 (MQ=255)                aCCAGCCACGGCCCGGCGATGATCAGCGTGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGa                                                                                                                          <  5:63779/148‑1 (MQ=255)                    gCCACGGCCCGGCGATGATCAGCGTGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCCCCAACAGGCTCCGCACCACCAGCACGTCGACCa                                                                                                                       >  4:1887/1‑147 (MQ=255)                       aCGGCGCGGCGATGATCTTGGTGATCAGCATCACCAGCAGGCGCGGCAAATAGCTCTGGGTCTGTTCGTTGATCTGCGTGGCCGCCTTGAAGATCGCCACCAGCAGGCCCACGATCTGGCTCCTCAACACCAGCACGGCGACCATGa                                                                                                                    <  8:63136/147‑1 (MQ=255)                        cGGCCCGGCGATGATCAGCGTGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAgg                                                                                                                  <  7:70436/148‑1 (MQ=255)                           cccGGCGATGATCAGCGTGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGggtg                                                                                                               <  8:83571/148‑1 (MQ=255)                                           gTGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCAt                                                                                               <  4:148648/148‑1 (MQ=255)                                            tGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATc                                                                                              >  1:150116/1‑148 (MQ=255)                                                       aCCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACCGGGCATCACGGAACAGGt                                                                                   >  7:61823/1‑148 (MQ=255)                                                         cagcagGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGACCATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGA‑GGTGGTCCGCCACAGGGCCTCACGGGACAGGGCgc                                                                                >  3:33673/1‑147 (MQ=255)                                                           gcagGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCg                                                                                 >  2:17818/1‑146 (MQ=255)                                                             agGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAg                                                                             <  1:49117/148‑1 (MQ=255)                                                               gcgcGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCg                                                                           >  3:5814/1‑148 (MQ=255)                                                                    gCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTc                                                                      <  4:5814/148‑1 (MQ=255)                                                                                          gTTGATCTGCGTGGCCGCCTGGCACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCCCCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCt                                                >  7:116267/1‑148 (MQ=255)                                                                                          gTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCt                                                >  3:118267/1‑148 (MQ=255)                                                                                          gTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCt                                                >  6:6858/1‑148 (MQ=255)                                                                                             gATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGa                                             >  2:68817/1‑148 (MQ=255)                                                                                                            cTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGAcgccg                              >  1:40941/1‑148 (MQ=255)                                                                                                              ggAACATCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGAcgccgc                             >  4:108330/1‑147 (MQ=255)                                                                                                                aaCATCGCCACCACCAGGCCCACCAACAGGCTCCGCAACACCAGCTCGGCGACCATGAGGGTGGTCAGCCACAGGGCAGCTCGGAACAGGTCGACAGCGACTTCAGGTGTCATGGGGGGCTCTCCTTGACGGCGTCAGACGCCGCCGa                          <  8:131278/148‑1 (MQ=255)                                                                                                                    tCGCCACCACCAGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGACGCCGCCGAAAct                      >  3:142025/1‑148 (MQ=255)                                                                                                                        caccaccaGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGACGCCGCCGAAActgctg                  <  2:150116/148‑1 (MQ=255)                                                                                                                          ccaccaGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGACGCCGCCGAAACTGCTgg                 >  7:107176/1‑147 (MQ=255)                                                                                                                            accaGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGACGCCGCCGAAACTGCTGGCCa              >  8:24962/1‑148 (MQ=255)                                                                                                                              caGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCATTGACGGCGTCAGACGCCGCCGAAACTGCTGGCCAgg            >  6:12346/1‑148 (MQ=255)                                                                                                                               aGGCCCACCAACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGACGCCGCCGAAACTGCTGGCCAggg           <  5:6858/148‑1 (MQ=255)                                                                                                                                   ccaccaACAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGACGCCGCCGAAACTGCTGGCCAGGGTGc        >  7:109697/1‑147 (MQ=255)                                                                                                                                        aaCAGGCTCCGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGACGCCGCCGAAACTGCTGGCCAGGGTGCCCATGa  <  8:107176/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                           TCCATGAACTTCTGCACCAGCCACGGCCCGGCGATGATCAGCGTGATCAGCATCACCAGCAGGCGCGGCAAAAAGCTCAGGGTCTGTTCGTTGATCTGCGTGGCCGCCTGGAACATCGCCACCACCAGGCCCACCAACAGGCTCGGCACCACCAGCACGGCGACCATGAGGGTGGTCAGCCACAGGGCATCACGGAACAGGTCGACAGCGACTTCAGGTGTCATCGGCGGCTCTCCTTGACGGCGTCAGACGCCGCCGAAACTGCTGGCCAGGGTGCCCATGA  >  NC_002947/4947413‑4947695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑