Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	499,203	A→G	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	40.7 / NA	13	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/5);  total (8/5)

ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTA  >  NC_002947/499061‑499328                                                                                                                                               |                                                                                                                              aCCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGCCATACGGGTGCCCGAAACTTGCCGATGCCTATGTGGGAGCGGGCAt                                                                                                                          <  5:4786/148‑1 (MQ=255)     tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCggggcaggcc                                                                                                                      >  1:62022/1‑141 (MQ=255)        gCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                    >  7:98433/1‑147 (MQ=255)                          tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCctgctg                                                                                                 >  5:25732/1‑148 (MQ=255)                                aGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAATCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGTATGCCCGCGAACGGGCCTGGCCTGCTGAtctc                                                                                            <  8:127544/147‑1 (MQ=255)                                               aCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGcc                                                                            <  2:47249/148‑1 (MQ=255)                                                 tCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAACGGGGCTTGCCTGCTGATCTATATTGCTGAGCCGGCctc                                                                          >  4:123834/1‑148 (MQ=255)                                                               ggTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCGGCGGATCTCTATGGCTGAGCCGGCCTCTTAGCGGGCATGcc                                                            >  6:157160/1‑148 (MQ=255)                                                                  gAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCGGGCCTGGGGATCGCTATGGCTGAGCCGGCCTCTTCGCGGGCAAGCCCGc                                                         >  6:57944/1‑148 (MQ=255)                                                                                    caAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAACGGGCCTGGCCTGCTGATCTATATTGCTGAGCCGGCCTCTTCGCGGGCCTGCCCGCTCCCACACGTTTCGTGGt                                       >  8:116467/1‑148 (MQ=255)                                                                                       gCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCCCGAACCGGCCGGGCCGGCTGATCTCTATGGGTGCGCCCGCCTCGTCCCGGGCCTGCCCGCGCCCACAGGTTTCGTGGTTgg                                    >  8:70088/1‑148 (MQ=255)                                                                                                     gACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAACGGGCCAGGCCGGCGGATCTCTATGGCTGAGCCGGCCGCATAGCGGGCATGACCGCGCCCACCCGGTTGGTGGTTGGCTCGCCAGGGCAtt                      >  8:83865/1‑148 (MQ=255)                                                                                                                    gAAACTGGCCGAAGCCTATGGGGGCGCGGGCAGGCCCGCGAACGGGCCTGGCCGGCTGATCTCTCTGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATcc       <  3:46727/148‑1 (MQ=38)                                                                                                                         ttGCCGACCCCTATGTGGGAGCGGGCAGGCCCGCGAAGGGGCCGGGCCGGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCAGGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTa  <  8:87789/148‑1 (MQ=255)                                                                                                                                               |                                                                                                                              ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTA  >  NC_002947/499061‑499328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑