| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 95.8% | 154.5 / 0.3 | 48 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (26/20); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932494‑1932776 | cggcgTCTTCGAAGAGGATCTGGACATTCGTCAGCGGCTTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCGT‑CGCGGGCAAGCCCGTCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑G‑ccc < 1:76473/149‑2 (MQ=255) cGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCt > 4:27858/1‑149 (MQ=255) aaGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTg > 1:1376/1‑148 (MQ=255) gACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGcc < 3:95243/147‑1 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAACCCC‑CTCCAACAAAGGAGATGCGTACCCGGTAGGAGCCGGGGTT‑C‑CCGCGCAGGGCTGGAAGGCAGCCCCaa > 4:161925/1‑148 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc > 5:346845/1‑149 (MQ=255) cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTc > 7:104201/1‑148 (MQ=255) gCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TAc > 5:234081/1‑149 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCAGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAg > 8:48553/1‑149 (MQ=255) tctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGc > 7:265100/1‑149 (MQ=255) cACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTGCGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGa > 1:277659/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTTGG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACATGTACGGATCAGCGAt > 1:138064/1‑149 (MQ=37) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAg < 4:62391/144‑1 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAg > 3:62391/1‑144 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAg > 7:233889/1‑144 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATc > 3:154820/1‑149 (MQ=255) gAAAAACGCATTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCGTGTAGGTG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCg < 3:161925/149‑1 (MQ=37) aaaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTTGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCGCTGATCCgcg < 2:1376/149‑3 (MQ=37) gCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGt < 6:346845/149‑1 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTc > 5:132391/1‑149 (MQ=255) ggggCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTAATAAAAAGGATATGCGTACCCAGTAGGAG‑CGGGCTT‑GCCCGCGAACGGGTGCAAGGCACCCCCAAATTGTAGAC‑TAAGCGCATCATCGATCCGGCAGGGTGATGTTCAGCTc > 6:358321/1‑149 (MQ=37) gggCCGCTTTGGGGCCCTG‑CGGGGGAAACCCGG‑CTCCTAAAATGGTTATGCGGCCCGTGTCGTGG‑GGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc < 2:138064/149‑1 (MQ=255) gggCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc > 4:116355/1‑148 (MQ=255) gggCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGCCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc < 4:154820/149‑1 (MQ=255) ggCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa < 6:132391/149‑1 (MQ=255) cTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 2:277659/149‑1 (MQ=255) gCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag < 8:265100/148‑1 (MQ=255) cGGCCCTG‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca < 3:27858/149‑1 (MQ=255) ggCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTATAC‑TACGCGGATCAGCGATACGGAAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGACCTGGtt > 8:224164/1‑149 (MQ=38) cAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt < 7:48553/149‑1 (MQ=255) aaGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCt < 4:203148/137‑1 (MQ=255) aaGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCt > 3:203148/1‑137 (MQ=255) aaGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 8:104201/149‑1 (MQ=255) gCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 4:364084/1‑147 (MQ=255) gCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 6:42935/1‑147 (MQ=255) g‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTc > 4:379578/1‑149 (MQ=255) tACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa < 2:297713/113‑1 (MQ=255) tACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa > 1:297713/1‑113 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 2:307027/1‑149 (MQ=255) aacgATATGGGTCCCCTGTTGTCG‑GGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGATCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac < 5:358321/146‑1 (MQ=255) tGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 3:379578/148‑1 (MQ=255) gCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 2:150018/1‑149 (MQ=255) gCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 1:294070/1‑149 (MQ=255) gCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGG‑TTTCCAGCTCGATATGCACATCATCGtt > 8:131381/1‑149 (MQ=255) gTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg < 7:224164/149‑1 (MQ=255) ccTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa > 2:236161/1‑149 (MQ=255) tAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGG‑TTTCCAGCTCGATATGCACATCATCGTTGCCGATGt < 7:131381/147‑1 (MQ=255) aGGAG‑CGGGCTG‑GCCCGCGAAGGGATGCAATGCAGCCCCAAAGTGTCGAC‑GACGCGGATCCTCGATCCGGCAGGGTGATGCTCAGCTCCAGACTCGTGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 8:268087/149‑1 (MQ=255) g‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca > 5:307014/1‑148 (MQ=255) | CGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTT‑CGCGGGCAAGCCCG‑CTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAC‑TACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACA > NC_002947/1932494‑1932776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |