| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,126,647 | +C | intergenic (‑101/+48) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 91.9% | 112.8 / 3.6 | 37 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (13/21); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947/1126498‑1126788 | tGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑c < 4:155129/149‑1 (MQ=255) gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑c < 8:225050/147‑1 (MQ=255) ggggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑cccgc < 5:153566/149‑4 (MQ=255) ggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑cccgctc < 8:250438/149‑6 (MQ=255) cgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGGTTTAACC‑CGCAAGGGGAGGTTTTTGCGCCCTCGCCTTTTCGGGG‑T‑AAA‑CCGCGCCTACACcga > 6:33687/1‑149 (MQ=255) cgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTTACCGCGAATTGGGAGGTATAGCCACCCTAGCATTAGC‑GGGTT‑AAA‑CCGCTccaaaaaa > 1:320688/1‑141 (MQ=255) aaCAGGTCGAAGACGCTCAGTGACATGTCGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTATACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCa < 5:328964/149‑1 (MQ=255) aCGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGt < 3:360545/149‑1 (MQ=255) aGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATCGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCg < 1:339374/147‑1 (MQ=255) aGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCg < 5:165475/147‑1 (MQ=255) aCATGTGGACCTCTCGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAgg < 1:337739/149‑1 (MQ=255) aCATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAg < 4:24245/148‑1 (MQ=255) cATGTGGACCTCCTTCCAGGTTGTTGGGGGTACATTGAGCCGTGTAGGCGC‑GGTTTTACGCGCGAATGGTGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg < 8:115534/149‑1 (MQ=38) gACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTTAACCCCAAAGCGGAGGTATAGCCACCAAAGCAATAGC‑GGG‑TAAAA‑CCGCGACTAAACAGACCCCCCCCCCCCACCCACGGGGCGGGGAGGGCGAt > 8:33599/1‑147 (MQ=255) gACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTAt < 7:33599/147‑1 (MQ=255) ctcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTAACCCCGAATGGGGAGGTATAGCCACCAAAGCATTAGA‑CGG‑T‑AAACCCGCGCCAACACAGAGCCCCCCCCCCAAGCCCAGTGGCGGGGAGGGCTATAAGGc > 4:231505/1‑149 (MQ=255) cctGCCAGGTGGTTGGGGGCACATTGCGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATACCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGt < 5:33687/149‑1 (MQ=37) aGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGAt < 2:244098/149‑1 (MQ=255) ggTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATa < 2:320688/149‑1 (MQ=255) ggTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGACGCCTGATa < 4:276105/149‑1 (MQ=38) ttgttgGGTGCACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGACGCCTGATAGc < 4:147802/149‑1 (MQ=37) cccTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc < 1:13393/149‑1 (MQ=255) gggTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATGGCTCTCGATCGACGGGCAGGCGCACACCAGGtt > 4:66249/1‑149 (MQ=255) gAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATGGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGaa < 3:66249/149‑1 (MQ=255) gCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACg > 7:156591/1‑149 (MQ=255) gAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTg > 6:29310/1‑149 (MQ=255) gAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTg < 3:231505/149‑1 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAg < 2:202351/118‑1 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAg > 1:202351/1‑118 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc > 7:127916/1‑145 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAc < 8:127916/145‑1 (MQ=255) ccaccaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 5:261106/1‑149 (MQ=255) accaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc < 2:54183/145‑1 (MQ=255) accaTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc > 1:54183/1‑145 (MQ=255) caTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTc > 7:137453/1‑78 (MQ=255) caTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTc < 8:137453/78‑1 (MQ=255) aTCGCATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc > 7:51514/1‑149 (MQ=255) cATTCGC‑GGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc < 1:160227/149‑1 (MQ=255) gc‑gGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc > 6:95889/1‑147 (MQ=255) gc‑gGG‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCAAGGTGGCGGGCAGCGCTATCAGGAGTCATGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTAGCGGTCGCCGAACACGTTGTCGACCCGCCCGACCggcggc > 6:335265/1‑147 (MQ=255) g‑T‑AAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAc > 5:133963/1‑149 (MQ=255) | TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCGC‑GGG‑T‑AAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGTAC > NC_002947/1126498‑1126788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |