| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_002947 | 4,980,585 | +GGC | intergenic (‑24/+44) | PP_4387 ← / ← flgE | hypothetical protein/flagellar hook protein FlgE |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_002947 | = 4980585 | 1 (0.010) | 54 (0.810) +GGC |
49/278 | 0.5 | 98.2% | intergenic (‑24/+44) | PP_4387/flgE | hypothetical protein/flagellar hook protein FlgE |
| ? | NC_002947 | 4980586 = | 1 (0.010) | intergenic (‑25/+43) | PP_4387/flgE | hypothetical protein/flagellar hook protein FlgE | |||||
CAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTC > NC_002947/4980448‑4980730 | caGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgc > 5:329312‑M1/1‑138 (MQ=255) gCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaat > 8:50905‑M1/1‑129 (MQ=255) gTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattaca < 5:34394‑M1/149‑25 (MQ=255) gTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattac > 5:185272‑M1/1‑125 (MQ=255) cAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacac > 6:229089‑M1/1‑120 (MQ=255) cAGGCTTATCCATTTATATCACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaat < 3:80295‑M1/146‑27 (MQ=255) cAGGCGGTGCCATCGACACAACGGGGCGGGCGCCGCGGGG‑GCAGGCAGAACCAGCACCAGCACGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGGGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacac < 7:180571‑M1/149‑30 (MQ=255) aGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacac > 4:197764‑M1/1‑119 (MQ=255) tttGCCATTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgaggccccaattacaaacaccaaag > 2:268896‑M1/1‑115 (MQ=255) aTTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAAAGACAGCGGCTTCAATCGCGTGAATATAGAACATTATTGGgggaggtttgcggccccaattacaaacaccaaatccaa > 1:129218‑M1/1‑110 (MQ=255) gAACGGGGCGGGGGCAGCGGGGTCCAGGCAAAACCAGCACAAACAAGAAAAACAAGGCCAGTAACCACAGCCGGTTAATACGGGTGAACCG‑TAAAATTATTGGGCCCGGTTGGCGGCCCCAATAACAAAGACCAAAGCCAATaaaaa > 7:247471/1‑146 (MQ=255) gAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatca > 4:324564‑M1/1‑102 (MQ=255) gcgggggcagcgggg‑gcaGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtca < 7:50905‑M1/149‑55 (MQ=255) cgggggcagcgggg‑gcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcat < 5:344556‑M1/149‑56 (MQ=255) gggggcagcgggg‑gcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatc < 6:329312‑M1/149‑57 (MQ=255) gggggcagcgggg‑gcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TAACATTCTTGGggccgctttgcggccccaattacaaacaacaaagccaatacaatcatcaggtcatc > 5:368230‑M1/1‑93 (MQ=255) ggtgcagcgggg‑tcaggcaGAACCAGCACCAGCAAGGTTAACAAGGACAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatct < 5:296610‑M1/146‑58 (MQ=255) agcgggg‑gcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaat > 3:367537‑M1/1‑87 (MQ=255) agcgggg‑gcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAAACT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcagctcatctgaat > 6:305972‑M1/1‑87 (MQ=255) gggg‑tcagGCAGAACCACCACCATCAAGGTTAACAAGGCCATTAACGACATACGATTCATTCGCGTGAAACT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatg < 5:317209‑M1/149‑66 (MQ=255) gggg‑gcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccggtttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatg < 5:229089‑M1/149‑66 (MQ=255) gcaggcagAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagc > 1:157445‑M1/1‑80 (MQ=255) gcaggcagAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagc < 2:157445‑M1/115‑36 (MQ=255) aggcagAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgc < 6:185272‑M1/149‑72 (MQ=255) gcagAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaacccaatacaatcataaggtcatctgaaagatggtctgcat > 8:324857‑M1/1‑76 (MQ=255) gcagAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattac > 4:273629‑M1/1‑76 (MQ=255) gcagAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattac < 3:273629‑M1/99‑24 (MQ=255) accatcaccagcAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatg < 7:88138‑M1/148‑78 (MQ=255) ccagcaccagcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggt > 4:79523‑M1/1‑70 (MQ=255) cagcaccagcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatc > 1:107796‑M1/1‑69 (MQ=255) cagcaccagcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatc < 2:107796‑M1/117‑49 (MQ=255) accagcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgcttt < 3:197764‑M1/149‑85 (MQ=255) ccagcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgcttt > 4:181947‑M1/1‑64 (MQ=255) cagcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacgaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttca > 7:208800‑M1/1‑63 (MQ=255) ggggAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtgga > 6:313793‑M1/1‑57 (MQ=255) aGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtctt < 6:368230‑M1/149‑101 (MQ=255) cGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttgg > 7:222734‑M1/1‑32 (MQ=255) cGCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttgg < 8:222734‑M1/134‑103 (MQ=255) gCTTCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagtt < 3:79523‑M1/149‑119 (MQ=255) ttCATTCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagcgaatacaatcatcaggtcatctgaatgatggtctgcatgatggcgctttcagtggaaatggtcttggcattggcctggtagttgc > 6:199110‑M1/1‑29 (MQ=255) ctatcgcGTGAACCT‑TCACAATCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctcaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgc < 2:176899‑M1/144‑121 (MQ=255) ttCGCGTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctg > 7:233139‑M1/1‑25 (MQ=255) gcgTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcc > 8:82637‑M1/1‑22 (MQ=255) gcgTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcc < 7:82637‑M1/132‑111 (MQ=255) gTGAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgcct < 3:181947‑M1/149‑130 (MQ=255) gAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctggatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttg > 6:240195‑M1/1‑18 (MQ=255) gAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctc > 3:177413‑M1/1‑18 (MQ=255) gAACCT‑TCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctc < 4:177413‑M1/140‑123 (MQ=255) aCCT‑TCACATTCTTGGggccgctttgcggccccaattaaaaacaccaaagccaatacaatcataaggtcttctgaatgatggtctgcatgatggtgctttaagtggaaatggtgttggcattggcctggttgtttctgtgcgccttgat > 7:333429‑M1/1‑16 (MQ=255) tCACATTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcagg > 8:18364‑M1/1‑12 (MQ=255) acaTTCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggt < 5:305972‑M1/148‑139 (MQ=255) caTTCTTGGggccgctttgcggtcccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctg < 3:273900‑M1/133‑125 (MQ=255) caTTCTTGGggccgctttgcggtcccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctg > 4:273900‑M1/1‑9 (MQ=255) ttCTTGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttac < 7:324857‑M1/149‑143 (MQ=255) gggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccagctc > 1:135856‑M1/1‑1 (MQ=255) | CAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGG‑GCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCT‑TCACATTCTTGG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTC > NC_002947/4980448‑4980730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |