| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 441,326 | 0 | G | . | 28.1% | 73.4 / 28.0 | 32 | coding (207/1173 nt) | bioF | 8‑amino‑7‑oxononanoate synthase |
| Reads supporting (aligned to +/- strand): ref base G (16/7); new base . (7/2); total (23/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCACCGCGC > NC_002947/441179‑441468 | gCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑gg < 1:383903/149‑1 (MQ=255) ggCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑gggg < 3:151244/149‑1 (MQ=255) gCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑ggggg < 3:203752/149‑1 (MQ=255) aTTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑ggtggggg < 7:249482/149‑1 (MQ=255) ggAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCATGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGccg > 3:294291/1‑148 (MQ=255) cGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCGCGCACATg > 4:247958/1‑149 (MQ=255) gggCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGGCCGCGCACCTGGGAg > 5:309122/1‑149 (MQ=255) gggCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCTCGCCCCTGGTTGt > 1:147411/1‑149 (MQ=255) gCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCTCGCCCCTGGTAGTTg > 3:56385/1‑149 (MQ=255) ggAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGCGCGCTGGGGTGT‑GGGGGGGGGGGCGTCGCCCCGGGTAGTTGGcc > 5:77151/1‑149 (MQ=255) ggCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCAt > 6:7708/1‑149 (MQ=255) cccAGCGGTGGGGGGCCTTCGGCAGCAATCCCGACCGGGGCCTGGCCAACCCCCCCGAGGGTATCGCCGCCGGGCAGGCCGGCGCCGAGCGCGGGGGTG‑‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATc < 3:160502/148‑1 (MQ=255) cAGCGGTTGCTGGCCTTCTGCAGCAATGACTTCCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAg > 2:196340/1‑149 (MQ=255) gcgggCGTTTTGCATCAATGACAACCTGGGCCGGGCCACCCCCCCCGCGTTGTTCGCCGCCTGGCTGGCGGGCGGCGTGCGGTGGGGGGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAag < 4:131210/146‑1 (MQ=255) gcgggCCTTGTGCCCCAATGACTACCTGGGCCGGGCCAACCCCCCCGTGGGGTTCGCCGCCGGGCAGGCCGGCCCCGCGGGGGGGGGTGG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAag < 8:110081/146‑1 (MQ=255) tGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGCCGAGCCACGGGTTAGTTGGCCCCCGCCCCCCCCACAAAAGGGTTGAaggg > 2:57952/1‑147 (MQ=255) tGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGGCATGGAACCTGTTTGTTGCCCCACGCCCCCCGCCCCAAAAGGTTGAAGAgg > 6:222836/1‑149 (MQ=255) gcagcaATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCTCGCACGTGGTATTTGGCCACACCACCCCGCACCATCAGGTTGAAGAGGCCCTggag > 3:199200/1‑147 (MQ=255) aCCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCGCCCACCTGGTAGTTGGCCCCAGCCCCCCGCACCCTAAGGTTGAAGAGGCACTGGCTGCACTCACCGgc > 3:324406/1‑149 (MQ=255) ccTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCACGCCCCGGGTAGTTGGCCCCAGCCCCCCGCACCAACAGGTTGAAGAGGCACTGGGGGAACTCACCGgcc > 5:156699/1‑149 (MQ=255) ccTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCAACAGGTTGAAGAGGCACTGGATGAACTCACCGgccg > 2:88017/1‑149 (MQ=255) ggccgcgCCAACCCCCCCGCGGTGCTCCCCCCCGGGCCGGCCGGGGGCGCCGGCGGGGGGGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGccgccc < 6:77151/143‑1 (MQ=255) cggcccAACCCCCCCGAGGGGAGCGCCGCCTGGCCGGCCGGCGCCGAGCGC‑GGGGGGG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCGGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCcgcgc < 7:160446/145‑1 (MQ=255) gCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCCCCAGCGGGTTGAAGAGGCACTGGCTGACCTCCCCGGCCGCCCgcgcgc > 8:160446/1‑148 (MQ=255) cAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCt > 1:335050/1‑149 (MQ=255) ccccGAGGTGTAGGCCGCCGTGCAGGCCGGGGCCGAGGGCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTgttgtt < 5:222836/149‑1 (MQ=255) ccccGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTgttgtt < 1:196340/149‑1 (MQ=255) cGAGGTGATCGCCGCCTGGCAGGCCGGCCCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCTCGCCCCTGGTTGTTGGCCACAGCACCCCGCACAATCAGGTTGAAGAGGCACTGGGTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTtct > 1:226595/1‑149 (MQ=255) cgccgcCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACCGCACCCCGCCCCATCAGGTTGAAGAGGCACTGGGTGAAATCACCGGCCGCCCGCCCGCCCTGTTGTTGTCCACCGGc > 4:131896/1‑148 (MQ=255) tGGCATGCCGGCGCTGTGGGGTGGTGTTG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGc < 4:324406/149‑1 (MQ=255) aGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACCGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAAt > 3:143019/1‑149 (MQ=255) cGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCCCAGCCCCCCGCCCCATCAGGTTGAAGAGGCACTGGGTGAACTCACCGGCCGCCCGCGCGCCCTGGTGTTCTCCCCCGGCTATTTGGCCAATCTgg > 3:45070/1‑149 (MQ=255) cGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTAGCCCCCGGCTATATGGCCAATCTggg > 4:267446/1‑149 (MQ=255) gcgcGGGGGGGG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCa < 4:56385/149‑1 (MQ=255) gggTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACCGCCCCCCGCACCATCAGGTTGAAGAGGCCCTGGGTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTATCCACCGGCTATATGGCCAATCTGGGCGCCATCCCCgcgc > 2:90346/1‑149 (MQ=255) gggTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCACCgcgc > 5:163370/1‑149 (MQ=255) | GCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCCACCGGCTATATGGCCAATCTGGGCGCCATCACCGCGC > NC_002947/441179‑441468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |