Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,921,460	G→A	G3494R (GGG→AGG)	PP_2561 →	hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,921,460	0	G	A	100.0%	108.4 / NA	34	G3494R (GGG→AGG)	PP_2561	hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/13);  total (21/13)

ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCA  >  NC_002947/2921314‑2921601                                                                                                                                                    |                                                                                                                                              acacGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagg                                                                                                                                             <  4:101645/149‑1 (MQ=255)         cggggggACCCCCGTAACGACAACCTGAACGGGCGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCgg                                                                                                                                     <  4:384621/147‑1 (MQ=255)           gggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGGAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGc                                                                                                                                    >  6:299290/1‑148 (MQ=255)            ggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCaa                                                                                                                                  >  6:277099/1‑149 (MQ=255)              ggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCAATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAAc                                                                                                                                 >  7:47424/1‑148 (MQ=255)                    cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCGAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                           >  1:99305/1‑149 (MQ=255)                    cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                           >  3:245108/1‑148 (MQ=255)                    cGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                           >  6:213288/1‑148 (MQ=255)                              aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGc                                                                                                                <  4:245108/149‑1 (MQ=255)                                 cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                              >  2:91519/1‑148 (MQ=255)                                    aaCGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCAATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAgg                                                                                                          <  8:47424/149‑1 (MQ=255)                                         gggCGCTGGCGATGACAGCCTGGATGGCGGC‑ATCGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTTGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACg                                                                                                     <  4:134790/149‑1 (MQ=255)                                                 gCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTACCGATCCggtg                                                                                             >  2:138925/1‑149 (MQ=255)                                                  cGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtgg                                                                                            >  1:339993/1‑149 (MQ=255)                                                    aTGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGgtggtg                                                                                          >  2:274105/1‑149 (MQ=255)                                                        cAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg                                                                                      <  5:277099/149‑1 (MQ=255)                                                         aGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGACAGGTAACGATACGgtggtgatggg                                                                                     >  6:269391/1‑144 (MQ=255)                                                           ccTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTgggg                                                                                   >  5:7882/1‑149 (MQ=255)                                                               gATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCg                                                                                <  2:385014/148‑1 (MQ=255)                                                                 tGGCGGC‑AACGGCAATGACACCCTGCACGGCGGCGATGGTCACGACACATTGGTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGt                                                                              <  6:348618/148‑1 (MQ=255)                                                                            ggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                   >  4:171579/1‑149 (MQ=255)                                                                                  gACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCt                                                                                                                         >  7:48195/1‑89 (MQ=255)                                                                                  gACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCt                                                                                                                         <  8:48195/89‑1 (MQ=255)                                                                                   acacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGc                                                             <  2:339993/148‑1 (MQ=255)                                                                                           cAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt                                                      <  5:213288/147‑1 (MQ=255)                                                                                                       ggTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCg                                                              <  4:30390/127‑1 (MQ=255)                                                                                                       ggTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCg                                                              >  3:30390/1‑127 (MQ=255)                                                                                                          aaCGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCa                                                                                                                       <  4:199415/67‑1 (MQ=255)                                                                                                          aaCGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCa                                                                                                                       >  3:199415/1‑67 (MQ=255)                                                                                                            cGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGt                                   >  1:346085/1‑149 (MQ=255)                                                                                                              acacATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTc                                 >  6:332482/1‑149 (MQ=255)                                                                                                                acaTTGTTCGGCGACGTGGTTACATACACCCTGATCAGGTGGGCCGGCAACTACTTCCTCAACTTTTCGGAAGGTAACGATACGGTGGTGGGTTGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGc                               <  2:99305/149‑1 (MQ=255)                                                                                                                     gTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg                          >  3:94879/1‑149 (MQ=255)                                                                                                                                 gggTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAAc               >  8:27755/1‑148 (MQ=255)                                                                                                                                             ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGCTCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACGTGAtcatca  >  4:206132/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                              ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGC‑AACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCA  >  NC_002947/2921314‑2921601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑