| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 3,951,159 | T→C | intergenic (‑123/+72) | PP_3486 ← / ← PP_3487 | cytochrome c/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,951,159 | 0 | T | C | 96.8% | 90.2 / ‑4.5 | 31 | intergenic (‑123/+72) | PP_3486/PP_3487 | cytochrome c/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (17/13); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01 | |||||||||||
AGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGG > NC_002947/3951021‑3951299 | aGGTCGACGGTGCCATGCCACGCACGCCGGGCAGCCATATCGCAAGCCCGGGGGGCGCGGCCTGGCGGCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGGGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACg < 6:391627/149‑1 (MQ=255) gCAGGAACGCCATATCCCACGCCCTGGGGGCGCGGCCTTGCGTCGCGAACGGGCTGCGCCGCAGCCCCGCCAGGGCCCGCTCAAGCGCACAAAGAGGGGCGCGCGGCGCGCG‑CCTGTCGCGACGCAAGGCCGCTCCCACAAAAGCGGAt < 1:44666/149‑1 (MQ=255) atatCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGc > 5:401470/1‑119 (MQ=255) atatCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGc < 6:401470/119‑1 (MQ=255) ggggAGCGGCCTTGCGTCGCTAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCACAAAGAGGGGCGCGCTGCGCGCT‑CCTTTCGCTACGCATGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAggcgg < 1:72950/148‑1 (MQ=39) cGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCAt > 5:162730/1‑148 (MQ=255) gCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCa > 1:79695/1‑149 (MQ=255) gTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTcc > 4:49455/1‑95 (MQ=255) gTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCacc > 2:120292/1‑149 (MQ=255) cgAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCca < 1:143693/93‑1 (MQ=255) cgAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCca > 2:143693/1‑93 (MQ=255) gAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCAAGATACTCaccgaccg > 1:320607/1‑149 (MQ=255) aGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGc > 5:206849/1‑148 (MQ=255) tGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCgg > 3:104373/1‑149 (MQ=255) agcagcCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTggg < 2:79695/149‑1 (MQ=255) ccgcCGCGGCAGTGGCAGCTAAAGCGCATAAAGTGGGCAGCGCTGCGCGCG‑CCTTTCCCTACGCATGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTc < 3:122618/147‑1 (MQ=255) ccGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTGTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCaa > 1:294016/1‑147 (MQ=255) cAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATgcgc < 4:104373/149‑1 (MQ=255) cAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCAAGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATgcgc < 2:320607/149‑1 (MQ=255) cTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTgg < 2:294016/149‑1 (MQ=255) aGAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATcgcc > 2:286849/1‑149 (MQ=255) gAAAGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATcgccg > 2:212861/1‑149 (MQ=255) aaGAGGGGAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAAATCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCt > 2:37926/1‑149 (MQ=255) gaaaggAGCGCGCAGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTgg > 7:70144/5‑149 (MQ=255) aaaggAGCGCGCAGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCt > 3:277409/4‑123 (MQ=38) aaaggAGCGCGCAGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCt < 4:277409/120‑1 (MQ=38) ggggAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCg > 2:27762/1‑149 (MQ=255) ggggAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCg > 7:156814/1‑149 (MQ=255) gggAGCGCTGCGCGCT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCgg > 6:401939/1‑149 (MQ=255) ggcgcgcggcgcgCT‑CCTTGCGCGACGCATGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGt < 4:356924/146‑1 (MQ=255) cagcgcT‑CCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCgg < 8:70144/147‑1 (MQ=255) | AGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCT‑CTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGG > NC_002947/3951021‑3951299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |