Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,468,078 Δ1 bp 9.1% coding (814/1047 nt) mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,468,0780T. 9.1% 166.5 / 11.7 55coding (814/1047 nt)mltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base T (23/27);  new base . (2/3);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

GAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATGGC  >  NC_002947/5467938‑5468224
                                                                                                                                             |                                                                                                                                                  
gAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtga                                                                                                                                            <  2:43024/149‑2 (MQ=255)
 aaGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGACACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATCAACCGACCATCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCggtcgt                                                                                                                                           >  3:182391/1‑149 (MQ=255)
   gTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCggtggt                                                                                                                                           >  2:69798/1‑147 (MQ=255)
    ttcttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg                                                                                                                                        <  2:23034/149‑1 (MQ=255)
         cAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGc                                                                                                                                      <  7:73806/146‑1 (MQ=255)
          aGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtc                                                                                                                                  <  5:285075/149‑1 (MQ=255)
            gCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtc                                                                                                                                  >  4:8299/1‑147 (MQ=255)
            gCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtc                                                                                                                                  <  3:8299/147‑1 (MQ=255)
            gCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGA‑ACCATGCCCGTGGACACGCCTtcgt                                                                                                                                >  8:146770/1‑149 (MQ=255)
              ccATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtcgtcg                                                                                                                              <  4:317295/149‑1 (MQ=255)
              ccATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtcgtcg                                                                                                                              >  8:171554/1‑149 (MQ=255)
                aTCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgc                                                                                                                            >  6:40174/1‑149 (MQ=255)
                 tCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                         >  4:89930/1‑131 (MQ=255)
                 tCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                         >  4:218717/1‑131 (MQ=255)
                 tCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                         <  3:89930/135‑5 (MQ=255)
                 tCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                         <  3:218717/135‑5 (MQ=255)
                 tCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcg                                                                                                                           <  6:277058/149‑1 (MQ=255)
                   cAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcgcg                                                                                                                         >  6:218327/1‑149 (MQ=255)
                    aGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTAACGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcgcgc                                                                                                                        >  5:299334/1‑149 (MQ=255)
                            gggCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGc                                                                                                                                                  <  1:139626/115‑1 (MQ=255)
                            gggCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGc                                                                                                                                                  >  2:139626/1‑115 (MQ=255)
                             ggCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACTGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTcc                                                                                                               <  1:69798/149‑1 (MQ=255)
                               ccGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCAc                                                                                                             <  6:289366/149‑1 (MQ=255)
                                         ccTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGa                                                                                                   >  6:46584/1‑149 (MQ=255)
                                             gagaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACt                                                                                                 <  8:86036/147‑1 (MQ=255)
                                              agaCGGAAGGCGGTAACCGGCAGATCATCGGGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGAc                                                                                              <  7:146770/149‑1 (MQ=255)
                                                aCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACca                                                                                            >  2:100534/1‑149 (MQ=255)
                                                   gAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCacac                                                                                         >  1:196755/1‑149 (MQ=255)
                                                      ggCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCt                                                                                      >  2:258261/1‑149 (MQ=255)
                                                               cGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCTCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCa                                                                             <  2:85165/149‑1 (MQ=255)
                                                                 gCAGATCATCTCGCATCGAATCATGAACCGACCAGCCAATCCTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATCCCGGTGGTCAGGCCTTCCTCGGAGCGCTCACCTTACACCAATGCTCGAATAACCACACCCTCGCCGGCCAcc                                                                           >  8:305881/1‑149 (MQ=255)
                                                                   aGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCCCCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCa                                                                         >  6:256762/1‑149 (MQ=255)
                                                                         tcGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTg                                                                   >  2:77407/1‑149 (MQ=255)
                                                                                  gAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGaa                                                          <  1:100534/149‑1 (MQ=255)
                                                                                  gAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGaa                                                          <  7:171554/149‑1 (MQ=255)
                                                                                     tCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCATGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTa                                                       <  4:182391/149‑1 (MQ=255)
                                                                                       aTGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAg                                                      <  5:40174/148‑1 (MQ=255)
                                                                                          aaCCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTg                                                   <  6:299334/148‑1 (MQ=255)
                                                                                                   gCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc                                         <  3:180507/149‑1 (MQ=255)
                                                                                                    ccAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGcc                                        >  7:243435/1‑149 (MQ=255)
                                                                                                      aaGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGa                                      >  8:77047/1‑149 (MQ=255)
                                                                                                       aGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGAt                                     <  7:77047/149‑1 (MQ=255)
                                                                                                         cGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCGGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATgg                                   <  5:218327/149‑1 (MQ=255)
                                                                                                               cAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGt                             <  8:97489/149‑1 (MQ=255)
                                                                                                                aaCTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCCGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATg                                    >  7:314921/1‑141 (MQ=255)
                                                                                                                aaCTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCCGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATg                                    <  8:314921/141‑1 (MQ=255)
                                                                                                                     cccTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc                                         >  8:102580/1‑131 (MQ=255)
                                                                                                                     cccTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc                                         <  7:102580/131‑1 (MQ=255)
                                                                                                                      ccTACCGTTTTCACCGGC‑TCGATGACGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTCACCACACCCTCGCCGCCCACCCAGCCGTGGCGAATGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGCgtt                      >  2:30880/1‑149 (MQ=255)
                                                                                                                       cTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgttg                     >  1:339631/1‑149 (MQ=255)
                                                                                                                            gTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCa                <  2:196755/149‑1 (MQ=255)
                                                                                                                                  aCCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATAtt          <  2:339631/149‑1 (MQ=255)
                                                                                                                                   ccGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTg         <  1:77407/149‑1 (MQ=255)
                                                                                                                                    cGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACTCCCTCGCCGCCCACCCAGCCGTGGCGGTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGa        <  1:30880/149‑1 (MQ=255)
                                                                                                                                           gATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATGGc  >  6:120247/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
GAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCCCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGATATGGC  >  NC_002947/5467938‑5468224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑