Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,420,598 Δ1 bp 14.0% intergenic (+81/+26) ctpH → / ← PP_t62 methyl‑accepting chemotaxis protein CtpH/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,420,5980A.14.0% 142.9 / 19.5 50intergenic (+81/+26)ctpH/PP_t62methyl‑accepting chemotaxis protein CtpH/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base A (24/19);  new base . (4/3);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.79e-01

GGCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCTTG  >  NC_002947/2420456‑2420745
                                                                                                                                              |                                                                                                                                                    
ggCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑Gc                                                                                                                                               <  4:213217/149‑1 (MQ=255)
ggCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑Gc                                                                                                                                               >  6:132235/1‑149 (MQ=255)
       ccTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCttt                                                                                                                                        >  8:144411/1‑149 (MQ=255)
          ttgggggttGTCACGGCACTTGGGCGAAGGCAACCCCCGCCTGCCTGCCTGACTCGGTCTGCCGGACGTGCAGGGGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCTTtgtg                                                                                                                                     <  6:107740/142‑1 (MQ=255)
          gCATGGTTTGTCAGGGCAGTTGGGCTAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCTCGC‑CATGTGCGGGCTTtgtg                                                                                                                                     <  8:49408/149‑1 (MQ=255)
           cATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtgt                                                                                                                                      <  7:133077/147‑1 (MQ=255)
           cATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCTTtgtg                                                                                                                                     <  8:45190/148‑1 (MQ=255)
            aTGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGa                                                                                                                                    >  2:282725/1‑148 (MQ=255)
                      aGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTg                                                                                                                          <  6:206672/148‑1 (MQ=255)
                           aGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCg                                                                                                                      >  8:202195/1‑147 (MQ=255)
                            gTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGgag                                                                                                                   <  7:142003/149‑1 (MQ=255)
                                ggCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGAc                                                                                                               <  2:279921/149‑1 (MQ=255)
                                  cGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAg                                                                                                             <  5:132235/149‑1 (MQ=255)
                                      gTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGAtt                                                                                                         >  4:152089/1‑149 (MQ=255)
                                      gTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGAtt                                                                                                         >  2:226485/1‑149 (MQ=255)
                                         aCCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCg                                                                                                       >  5:51840/1‑148 (MQ=255)
                                          ccTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGaa                                                                                                     >  3:162226/1‑149 (MQ=255)
                                             cccGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTc                                                                                                                       <  5:336108/128‑1 (MQ=255)
                                             cccGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTc                                                                                                                       >  6:336108/1‑128 (MQ=255)
                                                gCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCg                                                                                               <  7:202195/149‑1 (MQ=255)
                                                     aGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGcc                                                                                            <  6:283878/147‑1 (MQ=255)
                                                         ccTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTAGAACCTGCGGCCTTCTCg                                                                                      >  5:30811/1‑149 (MQ=255)
                                                          cTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGt                                                                                     >  1:298586/1‑149 (MQ=255)
                                                             aCTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTccc                                                                                  >  4:199436/1‑149 (MQ=255)
                                                             aCTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTccc                                                                                  >  4:195114/1‑149 (MQ=255)
                                                               tCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCg                                                                                 >  7:97103/1‑148 (MQ=255)
                                                                 ggTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAAc                                                                              <  2:212418/149‑1 (MQ=255)
                                                                       cAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACgag                                                                           <  2:276355/146‑1 (MQ=255)
                                                                        aGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCTGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGAcgc                                                                       <  6:30811/149‑1 (MQ=255)
                                                                               gCAGGTGCCATAGATGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTccc                                                                                  <  1:213021/131‑1 (MQ=255)
                                                                               gCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTccc                                                                                  >  2:213021/1‑131 (MQ=255)
                                                                               gCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCt                                                                >  6:61423/1‑149 (MQ=255)
                                                                                   gTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAAc                                                                              >  2:145842/1‑131 (MQ=255)
                                                                                       cATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTgcgc                                                        <  3:152089/149‑1 (MQ=255)
                                                                                                tGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTcc                                                >  8:33084/1‑148 (MQ=255)
                                                                                                        cgcACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGAt                                             >  5:114675/1‑143 (MQ=255)
                                                                                                        cgcACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGAt                                             <  6:114675/143‑1 (MQ=255)
                                                                                                                    tttttCGCACACAACACAAAGCCCTCACATG‑GCGGGCTTTGTGACAAGTATTTGGTCGGAGAGACAGGATTCTAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGc                            <  7:144411/148‑1 (MQ=255)
                                                                                                                              ttgtcacaaAGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttttt                 >  5:113517/5‑149 (MQ=255)
                                                                                                                              acaacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttttt                 >  1:162194/1‑149 (MQ=255)
                                                                                                                                aacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGc               >  5:334493/1‑149 (MQ=255)
                                                                                                                                aacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGc               >  2:61936/1‑149 (MQ=255)
                                                                                                                                 tcacaaAGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCt              >  7:302404/2‑149 (MQ=255)
                                                                                                                                 acacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGTGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCt              <  1:282725/149‑1 (MQ=255)
                                                                                                                                  cacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTc             <  6:51840/149‑1 (MQ=255)
                                                                                                                                     aaaGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGc           >  8:315337/1‑148 (MQ=255)
                                                                                                                                     aaaGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGc           >  1:169366/1‑148 (MQ=255)
                                                                                                                                           cGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGc               <  2:338279/138‑1 (MQ=255)
                                                                                                                                           cGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGc               >  1:338279/1‑138 (MQ=255)
                                                                                                                                           cGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCt    >  7:316402/1‑149 (MQ=255)
                                                                                                                                             cacaTG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCttg  <  4:162226/149‑1 (MQ=255)
                                                                                                                                             cacaTG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCttg  <  8:97103/149‑1 (MQ=255)
                                                                                                                                              acaTG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGtttt                  >  6:215516/1‑132 (MQ=255)
                                                                                                                                              acaTG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGtttt                  <  5:215516/132‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
GGCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCTTG  >  NC_002947/2420456‑2420745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑