Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,997,292 Δ1 bp 9.1% intergenic (‑130/‑23) PP_3525 ← / → PP_3526 hypothetical protein/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,997,2920C. 9.1% 136.1 / 7.2 44intergenic (‑130/‑23)PP_3525/PP_3526hypothetical protein/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (20/20);  new base . (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

TATCTCATCAGGCGCATCCTCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGACTTCATCCTGCTCGAACACTTCTCGA  >  NC_002947/3997146‑3997438
                                                                                                                                                  |                                                                                                                                                  
tatCTCATCAGGCGCATCCTCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGgcca                                                                                                                                                  <  7:122079/149‑1 (MQ=255)
 atCTCATCAGGCGCATCCTCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGgccag                                                                                                                                                 >  5:43000/1‑149 (MQ=255)
  tctcATCAGGCGCATCCTCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGgccagc                                                                                                                                                >  7:194720/1‑149 (MQ=255)
      atcaGGCGCATCCTCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGccagccag                                                                                                                                             >  8:225962/1‑148 (MQ=255)
                  cTCACTGACGCGAGGTCAGCATAGCGGTAAAGCTCCACGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGTCTCAGCCACCGGGGAGATTGTCTTTCCTCCTGGCACCGTCCACACTGTGCGGGCCTGCCAGGAAGCGGAGTTCa                                                                                                                                <  2:115126/149‑1 (MQ=255)
                  cTCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGtt                                                                                                                                  >  7:209320/1‑147 (MQ=255)
                  cTCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGtt                                                                                                                                  >  2:12238/1‑147 (MQ=255)
                   tCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGCACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGG‑CAGCCAGGAAGCGGAGTTCa                                                                                                                                >  5:240709/1‑147 (MQ=255)
                   tCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAgat                                                                                                                                  >  7:13832/1‑144 (MQ=255)
                   tCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGG‑CAGCCAGGAAGCGGAGTTCa                                                                                                                                >  5:236391/1‑147 (MQ=255)
                     aaTGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCagag                                                                                                                             >  2:333198/1‑149 (MQ=255)
                      aTGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGt                                                                                                                            >  8:172340/1‑149 (MQ=255)
                      aTGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGt                                                                                                                            <  7:172340/149‑1 (MQ=255)
                         aCGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGc                                                                                                                          <  6:202944/148‑1 (MQ=255)
                         aCGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGc                                                                                                                          <  6:191671/148‑1 (MQ=255)
                                     cATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCAcgccacgcca                                                                                                             <  7:225962/149‑1 (MQ=255)
                                          cGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCa                                                                                                        <  8:101745/149‑1 (MQ=255)
                                          cGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGG‑CAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAg                                                                                                       <  6:236391/149‑1 (MQ=255)
                                          cGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGG‑CAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAg                                                                                                       <  6:240709/149‑1 (MQ=255)
                                                 gCACCAGGACAGGCTTGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGTGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCaaaa                                                                                                 <  3:294689/149‑1 (MQ=255)
                                                            ggCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCcacgccacgc                                                                                                               >  4:79611/1‑124 (MQ=255)
                                                                ggTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCaa                                                                                  >  7:24308/1‑149 (MQ=255)
                                                                       cTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTa                                                                           >  7:263113/1‑149 (MQ=255)
                                                                        tGAACACGACCTGTAATACGACACAGCCACCGGGGAGATAGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACCCCACGCCAGTTCAGCCTAAACACCAGCACCAGCAACAAGCTAc                                                                          >  5:87548/1‑149 (MQ=255)
                                                                          aaCACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACgg                                                                        <  2:269920/149‑1 (MQ=255)
                                                                           acacGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACgg                                                                        <  8:194720/148‑1 (MQ=255)
                                                                             acGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCt                                                                      >  5:161448/1‑148 (MQ=255)
                                                                               gACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCaa                                                                   <  6:43000/149‑1 (MQ=255)
                                                                               gACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCaa                                                                   <  8:209320/149‑1 (MQ=255)
                                                                                            acacAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGcc                                                      <  8:24308/149‑1 (MQ=255)
                                                                                                   cACCGGGGAGATAGTCTTTCCTCCGGGCACCGTCCACACTCTCCCGGCCAGCCAGCAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGccc                                               <  4:9227/149‑1 (MQ=255)
                                                                                                    aCCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCCGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCa                                              <  1:12238/149‑1 (MQ=255)
                                                                                                          gagaTTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCAcc                                        <  8:263113/149‑1 (MQ=255)
                                                                                                              ttGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTAt                                    <  6:93941/149‑1 (MQ=255)
                                                                                                                 tCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCgg                                 <  6:161448/149‑1 (MQ=255)
                                                                                                                   tttCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCaaaa                                                                 <  7:171222/115‑1 (MQ=255)
                                                                                                                   tttCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCaaaa                                                                 >  8:171222/1‑115 (MQ=255)
                                                                                                                     tcctccGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTc                              >  7:9120/1‑148 (MQ=255)
                                                                                                                       ctccGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTC‑AAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGACt                          >  6:292660/1‑149 (MQ=255)
                                                                                                                        tccGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGACt                          >  3:216926/1‑149 (MQ=255)
                                                                                                                        tccGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGACt                          >  2:14026/1‑149 (MQ=255)
                                                                                                                            ggCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTAc                                                                          <  3:140918/97‑1 (MQ=255)
                                                                                                                            ggCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTAc                                                                          >  4:140918/1‑97 (MQ=255)
                                                                                                                             gCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCTGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGACTTCa                       <  8:9120/147‑1 (MQ=255)
                                                                                                                                 cGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGCCTTCATCCTGc                 >  2:62133/1‑149 (MQ=255)
                                                                                                                                        acTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGACTTCATCCTGCTCGAaca          >  5:312015/1‑149 (MQ=255)
                                                                                                                                               gggCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGACTTCATCCTGCTCGAACACTtctc    >  2:295842/1‑148 (MQ=255)
                                                                                                                                                ggCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGACTTCATCCTGCTCGAACACTTCTCGa  >  2:97714/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TATCTCATCAGGCGCATCCTCAATGACGGGAGGTCAGCATAGCGGTAAAGCACCAGGACAGGCTGGTTTGCCTGAACACGACCTGTAATACGACACAGCCACCGGGGAGATTGTCTTTCCTCCGGGCACCGTCCACACTCTGCGGGCCAGCCAGGAAGCGGAGTTCAGAGTGCCCACGCCACGCCAGTTCAGCCAAAAGACCAGCACCAGCAACATGCTACGGCTCAAAACAAGCGGCGCCAATGCCCAGGCGCCCTATCGGGTCGACTTCATCCTGCTCGAACACTTCTCGA  >  NC_002947/3997146‑3997438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑