Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,289,242 Δ1 bp 8.0% coding (3345/3468 nt) PP_3761 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,289,2420G. 8.0% 156.9 / 7.5 50coding (3345/3468 nt)PP_3761two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base G (25/21);  new base . (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGTGTTGCGTTCGCTAGTCAAATTCGCTCCAGTTGCGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATG  >  NC_002947/4289102‑4289389
                                                                                                                                            |                                                                                                                                                    
ggTGTTGCGTTCGCTAGTCAAATTCGCTCCAGTTGCGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTgg                                                                                                                                               <  3:49517/148‑1 (MQ=255)
 gtgtTGCGTTCGCTAGTCAAATTCGCTCCAGTTGCGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCg                                                                                                                                             >  8:280060/1‑149 (MQ=255)
 gtgtTGCGTTCGCTAGTCAAATTCGCTCCAGTTGCGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCAT‑GCCTTGGCgg                                                                                                                                            >  8:188249/1‑149 (MQ=255)
 gtgtTGCGTTCGCTAGTCAAATTCGCTCCAGTTGCGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCAT‑GCCTTGCCgg                                                                                                                                            >  4:102216/1‑149 (MQ=255)
    ttGCGTTCGCTAGTCAAATTCGCTCCAGTTGCGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTc                                                                                                                                          <  5:161457/149‑1 (MQ=255)
                gTCAAATTCGCTCCAGTTGCGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATc                                                                                                                              <  1:262575/149‑1 (MQ=255)
                    aaTTCGCTCCAGTTGCGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCgg                                                                                                                            >  3:196899/1‑147 (MQ=255)
                                   cGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑cgcg                                                                                                          <  5:147315/149‑1 (MQ=255)
                                   cGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑cgcg                                                                                                          >  8:52113/1‑149 (MQ=255)
                                    ggCATCCAGAAACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACTCTGCTGGTCATCCTTCATGGCCTTGTCGGTCACGGCGATGATCGGCAGTTTGCTCCAG‑ctcg                                                                                                          <  1:260831/148‑1 (MQ=255)
                                      cAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGtt                                                                                                       <  7:280060/149‑1 (MQ=255)
                                                               aGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCgg                                                                                      >  2:153401/1‑141 (MQ=255)
                                                               aGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCgg                                                                                      <  1:153401/141‑1 (MQ=255)
                                                                ggCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTc                                                                              >  4:244874/1‑148 (MQ=255)
                                                                  cGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGaa                                                                           <  8:83383/149‑1 (MQ=255)
                                                                    gTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGCCGGATCAGGCGGGTGGCCTCGAAGc                                                                         >  8:5652/1‑149 (MQ=255)
                                                                      ccAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCTGGACGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGcc                                                                        >  1:283799/1‑149 (MQ=255)
                                                                      ccAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCg                                                                       >  2:182865/1‑149 (MQ=255)
                                                                       cAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGt                                                                      <  1:182865/149‑1 (MQ=255)
                                                                             gATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAg                                                                          <  2:31951/139‑1 (MQ=255)
                                                                             gATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAg                                                                          >  1:31951/1‑139 (MQ=255)
                                                                              aTCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCAtctc                                                               >  2:80672/1‑149 (MQ=255)
                                                                                 ggCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCg                                                             <  7:52113/148‑1 (MQ=255)
                                                                                   cTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGca                                                          >  8:19563/1‑149 (MQ=255)
                                                                                      ggCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGcatc                                                        <  7:19563/148‑1 (MQ=255)
                                                                                      ggCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGcat                                                         <  3:244874/147‑1 (MQ=255)
                                                                                        ccAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTTCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCatcatc                                                     <  5:107172/149‑1 (MQ=255)
                                                                                        ccAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGcatcat                                                      >  2:11261/1‑148 (MQ=255)
                                                                                        ccAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCatcatc                                                     <  4:196899/149‑1 (MQ=255)
                                                                                          aGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCAc                                                   >  4:45322/1‑149 (MQ=255)
                                                                                             tAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTc                                                >  6:223199/1‑149 (MQ=255)
                                                                                               aTCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGaa                                                                           <  7:11490/120‑1 (MQ=255)
                                                                                               aTCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGaa                                                                           >  8:11490/1‑120 (MQ=255)
                                                                                               aTCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTcc                                               >  6:229438/1‑148 (MQ=255)
                                                                                                tCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCAt                                             >  5:58709/1‑149 (MQ=255)
                                                                                                tCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCAt                                             <  5:223199/149‑1 (MQ=255)
                                                                                                  aTTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCa                                           <  6:58709/149‑1 (MQ=255)
                                                                                                   ttGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCAtcatca                                                    <  1:248867/139‑1 (MQ=255)
                                                                                                   ttGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCAtcatca                                                    >  2:248867/1‑139 (MQ=255)
                                                                                                      gCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCAT‑GCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCAc                                       <  7:188249/148‑1 (MQ=255)
                                                                                                      gCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCAT‑GCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCAc                                       <  3:102216/148‑1 (MQ=255)
                                                                                                       cACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAT‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCAcc                                      <  7:5652/149‑1 (MQ=255)
                                                                                                        aCCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCAc                                       >  1:189080/1‑147 (MQ=255)
                                                                                                              ttGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGa                               >  5:104444/1‑149 (MQ=255)
                                                                                                                     cAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATAtcgtc                        <  3:45322/149‑1 (MQ=255)
                                                                                                                                gTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTc               >  5:269789/1‑147 (MQ=255)
                                                                                                                                     ccTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGgcgc        >  6:282983/1‑149 (MQ=255)
                                                                                                                                      cTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGGGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCCCCAGGTCGATATCGTCGTGCTGCTCCAGGCGCt       >  2:195023/1‑149 (MQ=255)
                                                                                                                                       ttCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTc      >  5:255654/1‑149 (MQ=255)
                                                                                                                                           tGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATg  >  6:23926/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
GGTGTTGCGTTCGCTAGTCAAATTCGCTCCAGTTGCGGCAGCCAGACACGGATCAACGAGAACAGGCGGTCCAGGTCGATCGGCTTGGCCAGGTAATCATTGGCACCGGCTTGCAGGCAACGCTGCTGGTCATCCTTCATGGCCTTGGCGGTCACGGCGATGATCGGCAGTTTGCGCCAG‑CGCGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATG  >  NC_002947/4289102‑4289389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑