Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,468,073:1 +A 9.1% coding (819/1047 nt) mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,468,0731.A 9.1% 201.9 / 16.7 55coding (819/1047 nt)mltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base . (23/27);  new base A (2/3);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

TGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGA  >  NC_002947/5467928‑5468218
                                                                                                                                                   |                                                                                                                                                 
tGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGCACCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑Tc                                                                                                                                                 >  1:85165/1‑149 (MQ=255)
          gAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtga                                                                                                                                      <  2:43024/149‑2 (MQ=255)
           aaGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGACACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATCAACCGACCATCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCggtcgt                                                                                                                                     >  3:182391/1‑149 (MQ=255)
             gTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCggtggt                                                                                                                                     >  2:69798/1‑147 (MQ=255)
              ttcttcAGGCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAg                                                                                                                                  <  2:23034/149‑1 (MQ=255)
                   cAGGCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGc                                                                                                                                <  7:73806/146‑1 (MQ=255)
                    aGGCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtc                                                                                                                            <  5:285075/149‑1 (MQ=255)
                      gCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtc                                                                                                                            <  3:8299/147‑1 (MQ=255)
                      gCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtc                                                                                                                            >  4:8299/1‑147 (MQ=255)
                      gCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGA‑ACCATGCCCGTGGACACGCCTtcgt                                                                                                                          >  8:146770/1‑149 (MQ=255)
                        ccATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtcgtcg                                                                                                                        >  8:171554/1‑149 (MQ=255)
                        ccATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTtcgtcg                                                                                                                        <  4:317295/149‑1 (MQ=255)
                          aTCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgc                                                                                                                      >  6:40174/1‑149 (MQ=255)
                           tCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                   >  4:89930/1‑131 (MQ=255)
                           tCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                   >  4:218717/1‑131 (MQ=255)
                           tCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                   <  3:89930/135‑5 (MQ=255)
                           tCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGCATCGA‑GCCggtgaaaa                                                                                                                                   <  3:218717/135‑5 (MQ=255)
                           tCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcg                                                                                                                     <  6:277058/149‑1 (MQ=255)
                             cAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcgcg                                                                                                                   >  6:218327/1‑149 (MQ=255)
                              aGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTAACGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGgcgcgc                                                                                                                  >  5:299334/1‑149 (MQ=255)
                                      gggCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGc                                                                                                                                            <  1:139626/115‑1 (MQ=255)
                                      gggCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGc                                                                                                                                            >  2:139626/1‑115 (MQ=255)
                                       ggCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACTGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTcc                                                                                                         <  1:69798/149‑1 (MQ=255)
                                         ccGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCAc                                                                                                       <  6:289366/149‑1 (MQ=255)
                                                    ccTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGa                                                                                             >  6:46584/1‑149 (MQ=255)
                                                        gagaCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACt                                                                                           <  8:86036/147‑1 (MQ=255)
                                                         agaCGGAAGGCGGTAACCGGCAGATCATCGGGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGAc                                                                                        <  7:146770/149‑1 (MQ=255)
                                                           aCGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACca                                                                                      >  2:100534/1‑149 (MQ=255)
                                                              gAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCacac                                                                                   >  1:196755/1‑149 (MQ=255)
                                                                 ggCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCt                                                                                >  2:258261/1‑149 (MQ=255)
                                                                          cGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCTCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCa                                                                       <  2:85165/149‑1 (MQ=255)
                                                                            gCAGATCATCTCGCATCGAATCATGAACCGACCAGCCAATCCTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATCCCGGTGGTCAGGCCTTCCTCGGAGCGCTCACCTTACACCAATGCTCGAATAACCACACCCTCGCCGGCCAcc                                                                     >  8:305881/1‑149 (MQ=255)
                                                                              aGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCCCCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCa                                                                   >  6:256762/1‑149 (MQ=255)
                                                                                    tcGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTg                                                             >  2:77407/1‑149 (MQ=255)
                                                                                             gAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGaa                                                    <  1:100534/149‑1 (MQ=255)
                                                                                             gAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGaa                                                    <  7:171554/149‑1 (MQ=255)
                                                                                                tCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCATGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTa                                                 <  4:182391/149‑1 (MQ=255)
                                                                                                  aTGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAg                                                <  5:40174/148‑1 (MQ=255)
                                                                                                     aaCCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTg                                             <  6:299334/148‑1 (MQ=255)
                                                                                                              gCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc                                   <  3:180507/149‑1 (MQ=255)
                                                                                                               ccAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGcc                                  >  7:243435/1‑149 (MQ=255)
                                                                                                                 aaGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGa                                >  8:77047/1‑149 (MQ=255)
                                                                                                                  aGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGAt                               <  7:77047/149‑1 (MQ=255)
                                                                                                                    cGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCGGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATgg                             <  5:218327/149‑1 (MQ=255)
                                                                                                                          cAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGt                       <  8:97489/149‑1 (MQ=255)
                                                                                                                           aaCTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCCGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATg                              >  7:314921/1‑141 (MQ=255)
                                                                                                                           aaCTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCCGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATg                              <  8:314921/141‑1 (MQ=255)
                                                                                                                                cccTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc                                   >  8:102580/1‑131 (MQ=255)
                                                                                                                                cccTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGc                                   <  7:102580/131‑1 (MQ=255)
                                                                                                                                 ccTACCGTTTTCACCGGC‑TCGATGACGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTCACCACACCCTCGCCGCCCACCCAGCCGTGGCGAATGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGCgtt                >  2:30880/1‑149 (MQ=255)
                                                                                                                                  cTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGgttg               >  1:339631/1‑149 (MQ=255)
                                                                                                                                       gTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCa          <  2:196755/149‑1 (MQ=255)
                                                                                                                                             aCCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATAtt    <  2:339631/149‑1 (MQ=255)
                                                                                                                                              ccGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTg   <  1:77407/149‑1 (MQ=255)
                                                                                                                                               cGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACTCCCTCGCCGCCCACCCAGCCGTGGCGGTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGa  <  1:30880/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGATCGCGTAGAAGTTCTTCAGGCCCATCCAGTACTCGGGGCCGCTCTCGC‑CCTCGAGACGGAAGGCGGTAACCGGCAGATCATCGCGCAGCGAATCATGAACCGACCAGCCAAGCGTACGCAACTCCCCTACCGTTTTCACCGGC‑TCGATGCCGGTGGTCAGGCCTTCGTCGGCGCGCTCACCTTCCACCCAGGCGCGACTGACCACACCCTCGCCGGCCACCCAGCCGTGGCGCTTGAAGTAGCTGGCCACGCTGCCGATGGCATCGTCAGGGTTGTTCCAGATATTGA  >  NC_002947/5467928‑5468218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑