Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,497,336:1 +G 7.7% coding (261/765 nt) PP_5562 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,497,3361.G 7.7% 198.3 / 11.4 52coding (261/765 nt)PP_5562hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (26/22);  new base G (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

CGAAGGCGTGGAAGCCCACCACCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGCGGACGGATCAGCGGTGCCACGGTG  >  NC_002947/3497191‑3497483
                                                                                                                                                  |                                                                                                                                                   
cGAAGGCGTGGAAGCCCACCACCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGc                                                                                                                                                  >  3:9915/1‑149 (MQ=255)
 gAAGGCGTGGAAGCCCACCACCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGc                                                                                                                                                  >  3:57771/1‑148 (MQ=255)
 gAAGGCGTGGAAGCCCACCACCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGc                                                                                                                                                  <  4:57771/148‑1 (MQ=255)
     gCGTGGAAGCCCACCACCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGaa                                                                                                                                             >  8:220727/1‑149 (MQ=255)
           aaGCCCAGCACCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGgcgc                                                                                                                                       <  4:292889/149‑1 (MQ=255)
           aaGCCCACCACCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGgcgc                                                                                                                                       <  2:181981/149‑1 (MQ=255)
            aGCCCACCACCCTGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGcgcg                                                                                                                                      <  8:147438/149‑1 (MQ=255)
              cccACCACCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGgc                                                                                                                                    <  3:9126/149‑1 (MQ=255)
               ccaccaCCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGgcc                                                                                                                                   >  4:41456/1‑149 (MQ=255)
               ccaccaCCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGgcc                                                                                                                                   >  4:305986/1‑149 (MQ=255)
                caccacCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCT‑GGTCACCGAGCTgg                                                                                                                                               <  4:245177/136‑1 (MQ=255)
                caccacCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCT‑GGTCACCGAGCTgg                                                                                                                                               >  3:245177/1‑136 (MQ=255)
                       cAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGGGGGAACAGTTCGGGGGTGGCGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGAt                                                                                                                           <  6:70904/149‑1 (MQ=255)
                            ggTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGtt                                                                                                                      >  4:160392/1‑149 (MQ=255)
                                  gCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTa                                                                                                                  >  1:329278/1‑147 (MQ=255)
                                             ggATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAgg                                                                                                     <  7:37503/149‑1 (MQ=255)
                                                tGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGTGAACAGTTCGGGTGTGGTGCTGATCAGGTTCAGCGTTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGcc                                                                                                  <  4:186639/149‑1 (MQ=255)
                                                   ccTGGCCGCTTTGTTCGAGTGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTgg                                                                                               <  3:68830/149‑1 (MQ=255)
                                                     tGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTgg                                                                                               >  1:285314/1‑147 (MQ=255)
                                                       gCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGt                                                                                           >  3:234355/1‑149 (MQ=255)
                                                         cGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTgg                                                                                         >  7:290286/1‑149 (MQ=255)
                                                         cGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTgg                                                                                         >  5:159949/1‑149 (MQ=255)
                                                              tgtTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTg                                                                                    <  7:220727/149‑1 (MQ=255)
                                                                ttCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTggg                                                                                  >  6:218038/1‑149 (MQ=255)
                                                                ttCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTggg                                                                                  >  2:309850/1‑149 (MQ=255)
                                                                ttCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTgg                                                                                   <  4:9915/148‑1 (MQ=255)
                                                                        tCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGAGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCgcgc                                                                          >  5:211986/1‑149 (MQ=255)
                                                                            gTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTg                                                                                                >  6:99619/1‑123 (MQ=255)
                                                                            gTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTg                                                                                                <  5:99619/123‑1 (MQ=255)
                                                                             tCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGACCGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCgct                                                                     >  8:81210/1‑149 (MQ=255)
                                                                               gTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTcgc                                                                            <  3:266668/140‑1 (MQ=255)
                                                                               gTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTcgc                                                                            >  4:266668/1‑140 (MQ=255)
                                                                                    ccAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTgcg                                                               <  3:321754/148‑1 (MQ=255)
                                                                                      aGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCtg                                                            >  8:54442/1‑149 (MQ=255)
                                                                                         gggAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTgc                                                         >  1:28150/1‑149 (MQ=255)
                                                                                            aaCAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGcc                                                      <  1:309850/149‑1 (MQ=255)
                                                                                               aGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTg                                                   <  2:285314/149‑1 (MQ=255)
                                                                                                gTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTg                                                   >  1:39537/1‑148 (MQ=255)
                                                                                                gTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGc                                                  <  8:290286/149‑1 (MQ=255)
                                                                                                gTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGc                                                  <  3:305986/149‑1 (MQ=255)
                                                                                                 ttCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCa                                                 <  3:41456/149‑1 (MQ=255)
                                                                                                  tCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAg                                                <  4:234355/149‑1 (MQ=255)
                                                                                                   cGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAg                                                <  7:219643/148‑1 (MQ=255)
                                                                                                             gCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCa                                      >  6:102218/1‑148 (MQ=255)
                                                                                                                       ttCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTg                           >  6:52404/1‑149 (MQ=255)
                                                                                                                           gCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGcgga                       >  7:26271/1‑149 (MQ=255)
                                                                                                                            cGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGcggac                      <  8:26271/149‑1 (MQ=255)
                                                                                                                                 cTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGCGGACGGAt                  >  2:339964/1‑148 (MQ=255)
                                                                                                                                  ttCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGCGGACGGAt                  >  3:201020/1‑147 (MQ=255)
                                                                                                                                  ttCCAGCT‑GGTCACCGAGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGcg                         >  3:234659/1‑140 (MQ=255)
                                                                                                                                  ttCCAGCT‑GGTCACCGAGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGcg                         <  4:234659/140‑1 (MQ=255)
                                                                                                                                               aCC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGCGGACGGATCAGCGGTGCCACGGt   >  3:5062/1‑149 (MQ=255)
                                                                                                                                                cc‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGCGGACGGATCAGCGGTGCCACGGTg  <  6:159949/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGAAGGCGTGGAAGCCCACCACCCAGCGGGTCGGGCTGGTGATGCGGATGACCTGGCCGCTGTGTTCCAGGGTCTTGTCGTATTCCAGCGGGAACAGTTCGGGGGTGGTGCTGATCAGGTTCAGCGGTACTTCCAGCTCGGTGACC‑AGCTGGAACGGCGCGGCCGAGCCGATACGTTCGTAGAGTTCGACCAGGGCCTGGAAGTGGCCCTGGGTTTCGCGCCCGCTGCTGCGCTGTGCGCCTTGCAGGTATTCGCCAAACAGGGTTTGCGGACGGATCAGCGGTGCCACGGTG  >  NC_002947/3497191‑3497483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑