Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	307,865:1	+C	100%	intergenic (+880/‑989)	hslO → / → PP_0254	molecular chaperone HslO/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	307,865	1	.	C	100.0%	201.0 / NA	58	intergenic (+880/‑989)	hslO/PP_0254	molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (30/28);  total (30/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAG  >  NC_002947/307723‑308004                                                                                                                                                |                                                                                                                                              aTGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgtta                                                                                                                                         <  8:255212/149‑6 (MQ=255) aTGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgtta                                                                                                                                         >  4:317393/1‑144 (MQ=255)         ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCg                                                                                                                                  <  3:317393/149‑1 (MQ=255)            gCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGTGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGt                                                                                                                                >  6:294552/1‑148 (MQ=255)            gCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccg                                                                                                                                            <  3:15412/135‑3 (MQ=255)            gCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccg                                                                                                                                            >  4:15412/1‑133 (MQ=255)             cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑c                                                                                                                                              <  3:58137/132‑1 (MQ=255)             cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑c                                                                                                                                              >  4:58137/1‑132 (MQ=255)                         ctgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCAc                                                                                                                    <  7:104253/147‑1 (MQ=255)                            ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTg                                                                                                               <  3:273957/149‑1 (MQ=255)                              ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTggg                                                                                                             >  4:90211/1‑149 (MQ=255)                              ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTggg                                                                                                             >  4:94985/1‑149 (MQ=255)                               gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                            <  3:18497/149‑1 (MQ=255)                                ctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGc                                                                                                           <  5:164180/149‑1 (MQ=255)                                 tgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGc                                                                                                           >  1:70491/1‑148 (MQ=255)                                 tgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGc                                                                                                           >  1:65626/1‑148 (MQ=255)                                   ccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCg                                                                                                          >  4:80018/1‑147 (MQ=255)                                     gATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAgg                                                                                                      >  5:136584/1‑149 (MQ=255)                                       tGTACTGCCTATGCACTGCGCGGCCAACATCGGTGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCg                                                                                                    <  5:294552/149‑1 (MQ=255)                                         tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGt‑t                                                                                                 <  2:143822/149‑1 (MQ=255)                                         tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGt‑t                                                                                                 <  2:199792/149‑1 (MQ=255)                                           cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑Tg                                                                                                >  2:241875/1‑148 (MQ=255)                                              ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCt                                                                                             >  6:188643/1‑148 (MQ=255)                                                     aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATg                                                                                     <  3:80018/149‑1 (MQ=255)                                                     aCTGCGCGGCAAACATCTGCTAAGCAGGCTATGTTACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTTATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TTTCTTCAACATg                                                                                     <  3:210572/149‑1 (MQ=255)                                                             gCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAg‑gcgg                                                                            <  6:136584/149‑1 (MQ=255)                                                              ccAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAg‑gcggc                                                                           <  3:103574/149‑1 (MQ=255)                                                                 aCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg                                                                                                       >  7:179909/1‑120 (MQ=255)                                                                 aCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAg                                                                                                       <  8:179909/120‑1 (MQ=255)                                                                 aCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGgctgc                                                                        <  5:188643/149‑1 (MQ=255)                                                                  cATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGa                                                                                                         <  3:70939/117‑1 (MQ=255)                                                                  cATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGa                                                                                                         >  4:70939/1‑117 (MQ=255)                                                                               ggCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCAt                                                          >  6:142773/1‑149 (MQ=255)                                                                                   aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGAc                                                      <  3:90211/149‑1 (MQ=255)                                                                                   aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGAc                                                      <  3:94985/149‑1 (MQ=255)                                                                                      tgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGgctgc                                                                        <  6:266088/128‑1 (MQ=255)                                                                                      tgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATAGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGgctgc                                                                        >  5:266088/1‑128 (MQ=255)                                                                                        aCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTc                                                 >  6:16074/1‑149 (MQ=255)                                                                                          cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                >  3:87266/1‑148 (MQ=255)                                                                                              ttcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaa                                           >  5:99045/1‑149 (MQ=255)                                                                                                 ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCgaga                                            <  7:240967/145‑1 (MQ=255)                                                                                                 ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCgaga                                            >  8:240967/1‑145 (MQ=255)                                                                                                       cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAGCTCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGc                                   <  1:241875/149‑1 (MQ=255)                                                                                                       cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGAAGACGAGCACGGTTGGGGCGAAGGCGTCCTTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGc                                   <  1:267492/149‑1 (MQ=255)                                                                                                          tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                 <  5:16074/147‑1 (MQ=255)                                                                                                          tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                 <  2:70491/147‑1 (MQ=255)                                                                                                          tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCg                                 <  2:65626/147‑1 (MQ=255)                                                                                                            cGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCa                             >  2:41706/1‑149 (MQ=255)                                                                                                              gCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATc                           >  7:170830/1‑149 (MQ=255)                                                                                                                        gACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCa                 >  5:324245/1‑149 (MQ=255)                                                                                                                        gACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCa                 >  8:251839/1‑149 (MQ=255)                                                                                                                         aCTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                <  7:251839/149‑1 (MQ=255)                                                                                                                          cTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAg‑gc                                                                              >  8:315584/1‑86 (MQ=255)                                                                                                                          cTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAg‑gc                                                                              <  7:315584/86‑1 (MQ=255)                                                                                                                          cTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                >  2:332154/1‑148 (MQ=255)                                                                                                                           tACCCTTACGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc               >  4:99139/1‑148 (MQ=255)                                                                                                                            aCCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc               >  3:231059/1‑147 (MQ=255)                                                                                                                             cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg            >  2:175514/1‑149 (MQ=255)                                                                                                                              ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAg            >  8:137816/1‑148 (MQ=255)                                                                                                                                  tCGGCTGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACg        <  1:41706/148‑1 (MQ=255)                                                                                                                                       tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg  >  3:212208/1‑149 (MQ=255)                                                                                                                                       tGACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg  >  2:111366/1‑149 (MQ=255)                                                                                                                                        gACGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGCGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg  >  5:256043/1‑148 (MQ=255)                                                                                                                                         aCGAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg  <  4:231059/147‑1 (MQ=255)                                                                                                                                                |                                                                                                                                              ATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGT‑TGTCTTCAACATGGAAG‑GCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAG  >  NC_002947/307723‑308004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑