Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,356,515 T→G 7.5% E749A (GAG→GCG)  infB ← translation initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,356,5150TG 7.5% 204.5 / 7.5 67E749A (GAG→GCG) infBtranslation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (29/33);  new base G (3/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

GCCTTCGAAGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGTTGAAGCCGAACAG  >  NC_002947/5356370‑5356663
                                                                                                                                                 |                                                                                                                                                    
gCCTTCGAAGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTcgc                                                                                                                                                   <  6:80107/149‑1 (MQ=255)
gCCTTCGAAGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTcgc                                                                                                                                                   <  2:226599/149‑1 (MQ=255)
   ttCGAAGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGaa                                                                                                                                                >  4:46042/1‑149 (MQ=255)
   ttCGAAGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGaa                                                                                                                                                >  8:5392/1‑149 (MQ=255)
   ttCGAAGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGa                                                                                                                                                 >  3:202488/1‑148 (MQ=255)
     cGAAGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGaa                                                                                                                                                <  5:102526/147‑1 (MQ=255)
      gAAGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACAt                                                                                                                                             <  5:36905/149‑1 (MQ=255)
        aGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCg                                                                                                                                           <  4:270413/149‑1 (MQ=255)
           tCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTg                                                                                                                                        <  4:199476/149‑1 (MQ=255)
              caACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGcc                                                                                                                                      >  6:165563/1‑148 (MQ=255)
               aaCGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGa                                                                                                                                    <  3:46042/149‑1 (MQ=255)
               aaCGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGa                                                                                                                                    <  3:310651/149‑1 (MQ=255)
               aaCGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGa                                                                                                                                    <  3:254994/149‑1 (MQ=255)
               aaCGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGa                                                                                                                                    <  4:202488/149‑1 (MQ=255)
                aCGTCATCACGCAGTACGCGAATCGGCCGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAAAGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAg                                                                                                                                   <  8:70030/149‑1 (MQ=255)
                   tcatcaCGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCAt                                                                                                                                <  3:251665/149‑1 (MQ=255)
                     atcaCGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGc                                                                                                                              >  5:320598/1‑149 (MQ=255)
                        aCGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCgg                                                                                                                           >  1:209920/1‑149 (MQ=255)
                        aCGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCgg                                                                                                                           >  1:214645/1‑149 (MQ=255)
                                   gAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCcttc                                                                                                                <  6:284792/149‑1 (MQ=255)
                                     aTCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATCCCGGTCAGGGCcttctt                                                                                                              <  8:116339/149‑1 (MQ=255)
                                                  ggTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCgatg                                                                                                 <  6:320598/149‑1 (MQ=255)
                                                  ggTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCgat                                                                                                  >  1:266133/1‑148 (MQ=255)
                                                  ggTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCgat                                                                                                  >  5:305434/1‑148 (MQ=255)
                                                   gTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCGCGAGAACATcctg                                                                                                                                         >  5:109395/1‑105 (MQ=255)
                                                   gTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCGCGAGAACATcctg                                                                                                                                         >  5:104564/1‑105 (MQ=255)
                                                   gTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCGCGAGAACATcctg                                                                                                                                         <  6:104564/108‑4 (MQ=255)
                                                   gTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCGCGAGAACATcctg                                                                                                                                         <  6:109395/108‑4 (MQ=255)
                                                         ggTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTACGGCGACACCCAGGATGTTCTCGCGAACATCCCTGCCGAGCATGCCGGTCACGGCCTTCTTGACGTCTTCGATGATGTCg                                                                                           >  8:283102/1‑148 (MQ=255)
                                                           tACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCg                                                                                           >  3:22284/1‑146 (MQ=255)
                                                                        cATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCgatgat                                                                                               >  6:181677/1‑129 (MQ=255)
                                                                        cATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCgatgat                                                                                               <  5:181677/129‑1 (MQ=255)
                                                                           aCAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAAc                                                                        >  3:120809/1‑149 (MQ=255)
                                                                            cagccagcGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAAc                                                                        <  7:141369/148‑1 (MQ=255)
                                                                                  gcGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATc                                                                 <  6:305434/149‑1 (MQ=255)
                                                                                       ttcgcCGAAGTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGAc                                                            >  3:217848/3‑149 (MQ=255)
                                                                                        gcgcCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGAc                                                            <  5:165563/148‑1 (MQ=255)
                                                                                             gAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCt                                                      <  7:5392/149‑1 (MQ=255)
                                                                                               aCTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGc                                                    <  2:266133/149‑1 (MQ=255)
                                                                                                 ttCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAAc                                                                        >  1:16213/1‑127 (MQ=255)
                                                                                                 ttCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAAc                                                                        <  2:16213/127‑1 (MQ=255)
                                                                                                 ttCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTc                                                  >  7:99810/1‑149 (MQ=255)
                                                                                                      cGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTcgacg                                              >  8:132590/1‑148 (MQ=255)
                                                                                                       gAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGAt                                            <  4:120809/149‑1 (MQ=255)
                                                                                                          cGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCtt                                         <  7:146657/149‑1 (MQ=255)
                                                                                                          cGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCtt                                         <  8:99810/149‑1 (MQ=255)
                                                                                                          cGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCATGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCtt                                         >  7:235971/1‑149 (MQ=255)
                                                                                                           ggAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTg                                        <  4:22284/149‑1 (MQ=255)
                                                                                                            gAACACGTCACGTACTTCGGCGACACCCATGATGTTCTAGCGAACATCTCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTgc                                       <  2:214645/149‑1 (MQ=255)
                                                                                                            gAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATACCGCATATCCAGACCTTCCTGCTCGACGATCTTgc                                       <  2:209920/149‑1 (MQ=255)
                                                                                                            gAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTgc                                       >  4:298720/1‑149 (MQ=255)
                                                                                                             aaCACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTgcg                                      <  1:171270/149‑1 (MQ=255)
                                                                                                                     acgtACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCg                               <  4:217848/148‑1 (MQ=255)
                                                                                                                       gtACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCa                            >  4:182253/1‑149 (MQ=255)
                                                                                                                       gtACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCa                            >  2:265674/1‑149 (MQ=255)
                                                                                                                         aCTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATc                          >  8:258922/1‑149 (MQ=255)
                                                                                                                          cTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCg                         >  5:212343/1‑149 (MQ=255)
                                                                                                                           ttCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCgg                        >  8:66412/1‑149 (MQ=255)
                                                                                                                               tcGACACCCATGATGTTCTCGCTAACATCGCTGCCGAGAATGCCGGTCAGGGCCTTCATGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGgcacg                    <  8:235971/148‑1 (MQ=255)
                                                                                                                                    acCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGt               >  6:5287/1‑149 (MQ=255)
                                                                                                                                      ccAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGtt              >  5:213361/1‑148 (MQ=255)
                                                                                                                                      ccAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGtt              >  1:277972/1‑148 (MQ=255)
                                                                                                                                      ccAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGtt              >  5:38964/1‑148 (MQ=255)
                                                                                                                                      cagcgATGTTCGCGAGAACATC‑CTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGTTg             >  4:325490/5‑148 (MQ=255)
                                                                                                                                       cAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGTTg             >  2:196539/1‑148 (MQ=255)
                                                                                                                                       cAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGTTGa            <  6:212343/149‑1 (MQ=255)
                                                                                                                                              ttCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGTTGAAGCCGaa     <  3:298720/149‑1 (MQ=255)
                                                                                                                                                 tcGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGTTGAAGCCGAACAg  >  1:296631/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCCTTCGAAGATCACAACGTCATCACGCAGTACGCGAATCGGACGGTTGCGGTACACGGTACCCTCGATGACCATACAGCCAGCGATGGCGCCGAACTTCGGCGAACGGAACACGTCACGTACTTCGGCGACACCCAGGATGTTCTCGCGAACATCGCTGCCGAGCATGCCGGTCAGGGCCTTCTTGACGTCTTCGATGATGTCGTAGATCACGTTGTAATAACGCATATCCAGACCTTCCTGCTCGACGATCTTGCGCGCACCGGCATCGGCACGCACGTTGAAGCCGAACAG  >  NC_002947/5356370‑5356663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑