Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,418,999 G→A 8.4% A24A (GCC→GCT)  PP_4758 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,418,9990GA 8.4% 131.5 / 2.6 49A24A (GCC→GCT) PP_4758MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (21/23);  new base A (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

ACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACA  >  NC_002947/5418858‑5419145
                                                                                                                                              |                                                                                                                                                  
aCCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTgg                                                                                                                                             <  5:1083/149‑1 (MQ=255)
 ccGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGc                                                                                                                                            <  7:76225/149‑1 (MQ=255)
    aaGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCaa                                                                                                                                         >  5:2319/1‑149 (MQ=255)
    aaGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCaa                                                                                                                                         <  8:204884/149‑1 (MQ=255)
     aGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCAGTGCCGTGGCCaag                                                                                                                                        <  2:174733/149‑2 (MQ=255)
        cATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAAcgcg                                                                                                                                     >  1:100682/1‑149 (MQ=255)
        cATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAAcgcg                                                                                                                                     >  8:244922/1‑149 (MQ=255)
         aTAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGt                                                                                                                                    >  8:234480/1‑149 (MQ=255)
             gCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGcc                                                                                                                                >  1:51223/1‑149 (MQ=255)
               cGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCtt                                                                                                                              <  2:141660/149‑1 (MQ=255)
                  aGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACAGCACTGGCCAACGCGTCGCCTTCAt                                                                                                                           >  2:128122/1‑149 (MQ=255)
                          tGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCa                                                                                                                   <  3:29734/149‑1 (MQ=255)
                            cccAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACAGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGa                                                                                                                 <  1:128122/149‑1 (MQ=255)
                                  tCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCg                                                                                                                                  >  7:312585/1‑126 (MQ=255)
                                  tCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCg                                                                                                                                  <  8:312585/126‑1 (MQ=255)
                                        tCCAGGTGCGGGCGGACGAAGCGAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAgg                                                                                                       <  3:321255/147‑1 (MQ=255)
                                        tCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAgg                                                                                                       <  6:2319/147‑1 (MQ=255)
                                           aGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTc                                                                                                  <  4:35277/149‑1 (MQ=255)
                                             gTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTGCACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCa                                                                                                                   <  1:71131/131‑1 (MQ=255)
                                             gTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCa                                                                                                                   >  2:71131/1‑130 (MQ=255)
                                             gTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGt                                                                                                >  4:319876/1‑149 (MQ=255)
                                                cgggcggACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCa                                                                                             >  3:96318/1‑149 (MQ=255)
                                                         gAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGAt                                                                                    >  5:255868/1‑149 (MQ=255)
                                                            gCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGAtctc                                                                                 <  4:96318/149‑1 (MQ=255)
                                                             ccAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTcc                                                                                <  6:54375/149‑1 (MQ=255)
                                                              cAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTcc                                                                                >  3:192923/1‑148 (MQ=255)
                                                                      aCGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTg                                                                       >  6:151578/1‑149 (MQ=255)
                                                                      aCGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACATGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTg                                                                       >  1:335295/1‑149 (MQ=255)
                                                                           gTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACAGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCa                                                                                             >  2:59744/1‑122 (MQ=255)
                                                                           gTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACAGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCa                                                                                             <  1:59744/122‑1 (MQ=255)
                                                                            tCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTtg                                                                 <  7:151186/149‑1 (MQ=255)
                                                                                    gTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGcgggtc                                                         >  3:323064/1‑149 (MQ=255)
                                                                                        aCGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGcgggtcgggt                                                     >  4:83612/1‑149 (MQ=255)
                                                                                         cGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGcgggtcgg                                                       <  7:94241/146‑1 (MQ=255)
                                                                                         cGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGcgggtcgg                                                       >  8:94241/1‑146 (MQ=255)
                                                                                                   gatgaCGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGgtgt                                                                                                   <  3:291998/92‑1 (MQ=255)
                                                                                                   gatgaCGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGgtgt                                                                                                   >  4:291998/1‑92 (MQ=255)
                                                                                                   gatgaCGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATg                                          >  4:285466/1‑149 (MQ=255)
                                                                                                    atgaCGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATg                                          <  2:100682/148‑1 (MQ=255)
                                                                                                      gaCGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGGGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATgg                                         >  5:254302/1‑147 (MQ=255)
                                                                                                         gAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCTCTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTg                                    <  4:265086/149‑1 (MQ=255)
                                                                                                             aGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTggg                                  >  1:322659/1‑147 (MQ=255)
                                                                                                                  cAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGGCGGTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGc                           <  7:244922/149‑1 (MQ=255)
                                                                                                                     gACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTa                        <  2:51223/149‑1 (MQ=255)
                                                                                                                           gcgcTTG‑ACCTTGGCCACGGCACTTGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCa                  <  6:255868/149‑1 (MQ=255)
                                                                                                                                    ccTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGtt          <  2:335295/149‑1 (MQ=255)
                                                                                                                                          ccACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGaa             >  7:73304/1‑140 (MQ=255)
                                                                                                                                          ccACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGaa             <  8:73304/140‑1 (MQ=255)
                                                                                                                                            aCGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACa  <  5:151578/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
ACCGAAGCCATAGGCCGCAGCGCTGATGCCCAGGTCGCTTTCCAGGTGCGGGCGGACGAAGCCAATGTTGACGCGGTCGAGGTAGTTGACGATGAACATGATGACGAACAGCGGCAGGACGTGGCGCTTG‑ACCTTGGCCACGGCACTGGCCAACGCGTCGCCTTCATGCCGGGCAGACGGAGCGAGGGTGTCGTTCACTTGCGGATCTCCCACTAATTGTTTTTGTGCGGGTCGGGTTGTCTGACATGGCAGTGGGGAGGAGCTTATTCGCAGTGAAGTTGTACGACA  >  NC_002947/5418858‑5419145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑