Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,472,882 G→T 9.8% L225I (CTA→ATA)  nadD ← nicotinate‑nucleotide adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,472,8820GT 9.8% 153.2 / 6.5 51L225I (CTA→ATA) nadDnicotinate‑nucleotide adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base T (3/2);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAATATTTCCCGCCGGGCCGGACATG  >  NC_002947/5472735‑5473030
                                                                                                                                                   |                                                                                                                                                    
cTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGt                                                                                                                                                     >  5:41374/1‑149 (MQ=255)
   gATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGTGCGCACGATATAGTTCg                                                                                                                                                  >  7:300976/1‑149 (MQ=255)
   gATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGtt                                                                                                                                                    <  7:291529/147‑1 (MQ=255)
     tGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTg                                                                                                                                                <  1:38030/149‑1 (MQ=255)
       tCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTTCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTgc                                                                                                                                               <  7:168608/148‑1 (MQ=255)
           gggCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAATCTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTc                                                                                                                                          >  7:22724/1‑149 (MQ=255)
            ggCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCt                                                                                                                                           <  5:17639/147‑1 (MQ=255)
             gCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGAACTATATCGTGCGcccaac                                                                                                                                        <  3:255151/149‑5 (MQ=255)
              ccTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGat                                                                                                                                       <  2:283857/149‑1 (MQ=255)
              ccTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGat                                                                                                                                       <  4:10852/149‑1 (MQ=255)
               cTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGata                                                                                                                                      >  5:203168/1‑149 (MQ=255)
                ttGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTTGTGCGCCTCGata                                                                                                                                      >  6:341772/1‑148 (MQ=255)
                  gACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAg                                                                                                                                   >  6:177821/1‑149 (MQ=255)
                    cGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGc                                                                                                                                 <  6:203168/149‑1 (MQ=255)
                     gTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGc                                                                                                                                 >  1:43239/1‑148 (MQ=255)
                           cAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACg                                                                                                                          >  6:283813/1‑149 (MQ=255)
                                   ccTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCgg                                                                                                                  >  4:134596/1‑149 (MQ=255)
                                               cGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAAcc                                                                                                       <  6:41374/148‑1 (MQ=255)
                                               cGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCt                                                                                                      >  5:68149/1‑149 (MQ=255)
                                                gACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTc                                                                                                     <  6:68149/149‑1 (MQ=255)
                                                   cAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCAcc                                                                                                  <  2:43239/149‑1 (MQ=255)
                                                     gTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGa                                                                                                >  1:59263/1‑149 (MQ=255)
                                                         ttcGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTg                                                                                            >  3:191430/1‑149 (MQ=255)
                                                         ttcGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGAACTATATCGTgcgc                                                                                                                                             >  2:156006/1‑100 (MQ=255)
                                                         ttcGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGAACTATATCGTgcgc                                                                                                                                             <  1:156006/100‑1 (MQ=255)
                                                           cGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGc                                                                                           <  4:191430/148‑1 (MQ=255)
                                                            gCCGTAAATTTTCTGCTTGGTCATATAAAAATCGATCAACTCGTTGCATGAGGCGCCAGAGGCACCGTCAGATGGGCGCACGATATACTTCGTGCGCATCGATATAGGCCAGTACGGAGTCCGGCACCAGGAACCTCAACGAttttcag                                                                                         >  7:265559/1‑145 (MQ=255)
                                                              cGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCt                                                                                       <  5:283813/149‑1 (MQ=255)
                                                                tAAATTTTTTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGTTGAGGAGCCAGAGGCACAGTCATTTGGGCGGACGATATAGTTAGTGCGCCTCGATATATGCGAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTg                                                                                      <  8:265559/148‑1 (MQ=255)
                                                                  aaTTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGc                                                                                    <  5:177821/148‑1 (MQ=255)
                                                                    ttttCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCcag                                                                                 >  5:169389/1‑149 (MQ=255)
                                                                                tCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGa                                                                      >  6:148828/1‑148 (MQ=255)
                                                                                  atatAAATCTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCt                                                                   <  8:22724/149‑1 (MQ=255)
                                                                                    ataAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCtgt                                                                 >  1:90388/1‑149 (MQ=255)
                                                                                        atcTCGTTCAACTAGTTGGATTAGTCGCCAGAGGCACCGGCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAg                                                             <  8:300976/147‑1 (MQ=255)
                                                                                             gTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGa                                                         >  5:659/1‑148 (MQ=255)
                                                                                                 aaCTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCg                                                    >  7:154160/1‑149 (MQ=255)
                                                                                                       ttGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAgcg                                              >  2:65499/1‑149 (MQ=255)
                                                                                                               ggCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGc                                      >  5:5469/1‑149 (MQ=255)
                                                                                                                   ccAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGa                                  >  6:257750/1‑149 (MQ=255)
                                                                                                                   ccAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGa                                  <  2:59263/149‑1 (MQ=255)
                                                                                                                            ccGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGacgaac                             <  1:47666/145‑1 (MQ=255)
                                                                                                                            ccGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGacgaac                             >  2:47666/1‑145 (MQ=255)
                                                                                                                              gTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAata                       >  6:321051/1‑149 (MQ=255)
                                                                                                                                 aGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAATAtt                     >  4:160917/1‑148 (MQ=255)
                                                                                                                                  gTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAATATTTc                   >  8:237563/1‑149 (MQ=255)
                                                                                                                                     aggCGCACGAACTATATCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAATATTTCccg                >  4:19108/2‑149 (MQ=255)
                                                                                                                                        cgcACGAACTATATCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAATATTTCccgccg             >  5:268598/1‑149 (MQ=255)
                                                                                                                                         gcACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAAAGAAATATTTCCCgccgg            >  5:99815/1‑149 (MQ=255)
                                                                                                                                               tataGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAATATTTCCCGCCGGGCCGGa      <  5:148828/149‑1 (MQ=255)
                                                                                                                                                  aGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAATATTTCCCGACGGGCCGGACAt   >  3:231098/1‑149 (MQ=255)
                                                                                                                                                   gTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAATATTTCCCGCCGGGCCGGACATg  >  5:282769/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGGATGTCCTGGGCCTTGACGTCTTCCAGCGCTGCCTTGGTCACTGCGACCAGTTCTTCGCCGTAAATTTTCTGCTTGGTCATATAAAACTCGTTCAACTCGTTGGATGAGGCGCCAGAGGCACCGTCAGTTGGGCGCACGATATAGTTCGTGCGCCTCGATATAGGCCAGTACGGCGTCCGGCACCAGGAACCTCACCGATTTGCCGCTGGCCAGCAGCTGTCGGATCTGTGTAGCCGACACCGCAAGCGGCGTCTGCCAGACGAACGAAATATTTCCCGCCGGGCCGGACATG  >  NC_002947/5472735‑5473030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑