Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,489,781	T→A	5.9%	M88K (ATG→AAG) ‡	PP_4825 →	MarC family membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,489,781	0	T	A	5.9%	194.0 / 2.9	68	M88K (ATG→AAG) ‡	PP_4825	MarC family membrane protein
Reads supporting (aligned to +/- strand):  ref base T (34/28);  new base A (2/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

CAACTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATGAAAAA  >  NC_002947/5489633‑5489926                                                                                                                                                      |                                                                                                                                                   caaCTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCAt                                                                                                                                                    <  6:272302/149‑1 (MQ=255)  aaCTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATg                                                                                                                                                   >  5:45951/1‑149 (MQ=255)   aCTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATgg                                                                                                                                                  <  1:104589/149‑1 (MQ=255)     tGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑cc                                                                                                                                               >  4:211747/1‑149 (MQ=255)        ccTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAg                                                                                                                                            <  1:305601/149‑1 (MQ=255)         gtGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAgg                                                                                                                                           >  5:193731/2‑149 (MQ=255)                  cgcgTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcgg                                                                                                                                  >  8:3350/1‑149 (MQ=255)                   gcgTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcggc                                                                                                                                 >  3:267239/1‑149 (MQ=255)                    cgTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcggcg                                                                                                                                <  7:311129/149‑1 (MQ=255)                     gTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcggcgg                                                                                                                               >  4:255526/1‑149 (MQ=255)                     gTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcggcgg                                                                                                                               <  3:75772/149‑1 (MQ=255)                         gCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTa                                                                                                                             >  6:292586/1‑147 (MQ=255)                         gCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTAc                                                                                                                            <  3:255526/148‑1 (MQ=255)                          cAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAg                                                                                                                          <  5:90393/149‑1 (MQ=255)                            gCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGc                                                                                                                        <  3:211747/149‑1 (MQ=255)                                  gTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACaa                                                                                                                  >  2:265583/1‑149 (MQ=255)                                     gCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCAGTACAGGCAGACAACGt                                                                                                               >  5:280597/1‑149 (MQ=255)                                       cAGCGTGTTGCTGTCCCTGTTCGGTCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTAATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTc                                                                                                             <  6:238663/149‑1 (MQ=255)                                       cAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGtt                                                                                                              >  7:297167/1‑148 (MQ=255)                                       cAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTc                                                                                                             <  4:115410/149‑1 (MQ=255)                                           gtgtTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTcagca                                                                                                         <  8:166778/149‑1 (MQ=255)                                             gtTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTcagcag                                                                                                        >  8:211215/1‑148 (MQ=255)                                              ttGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGa                                                                                                      <  5:166976/149‑1 (MQ=255)                                                gCTGTACCTGTTCGGGCGGGCTATCTTCGTAATTTTCGCCATCACCGCCGATGCCTTCCGCATGGGTGCTGGCTGCGGGCTGTTCATTTCGGCCC‑TGGGCAGGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGAt                                                                                                     <  6:31689/148‑1 (MQ=255)                                                 cTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGAtgt                                                                                                   <  1:214272/149‑1 (MQ=255)                                                    tACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGa                                                                                                 <  5:292586/148‑1 (MQ=255)                                                    tACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGAc                                                                                                <  7:31338/149‑1 (MQ=255)                                                    tACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGAc                                                                                                >  6:306512/1‑149 (MQ=255)                                                      ccTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCa                                                                                              >  3:221264/1‑149 (MQ=255)                                                       gttttggggcgggcGATTTGCTTATTTTGCGCCATCCCCCCGTTTGCCTGCCCCAGGGGGGGTGGCTGGGTG‑TGTTTATTTCCGCCCGTGGGCTGGG‑CCCTGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCAt                                                                                             <  6:45951/143‑1 (MQ=255)                                                          ttCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACa                                                                                                                           <  1:88088/116‑1 (MQ=255)                                                          ttCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACa                                                                                                                           >  2:88088/1‑116 (MQ=255)                                                          ttCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCg                                                                                           >  1:80506/1‑148 (MQ=255)                                                                   gcgATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGAc                                                                                 >  1:107817/1‑149 (MQ=255)                                                                   gcgATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTAATTTCGGCCC‑TGGGCATGG‑CCCTGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGAc                                                                                 <  8:147813/149‑1 (MQ=255)                                                                     gATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCa                                                                               >  6:172874/1‑149 (MQ=255)                                                                                  ttCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTCCCGCTGACCATTCCGCTGACCg                                                                   <  7:211215/148‑1 (MQ=255)                                                                                        aTCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCCATG‑CCCAggg                                                                                                                                          >  5:205132/1‑71 (MQ=39)                                                                                        aTCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCCATG‑CCCAggg                                                                                                                                          <  6:205132/71‑1 (MQ=39)                                                                                            ccgccgATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCgg                                                         >  7:252530/1‑148 (MQ=255)                                                                                                cgATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCa                                                    >  1:219408/1‑149 (MQ=255)                                                                                                       ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGAtgtg                                                                                                  >  7:307563/1‑96 (MQ=255)                                                                                                       ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGAtgtg                                                                                                  <  8:307563/96‑1 (MQ=255)                                                                                                       ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGc                                             >  6:130067/1‑149 (MQ=255)                                                                                                       ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGCCAAGCCGGCGGTACAGCCAGACAACGTACAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTAACCGTTGGCCCCGGCACCATCGGTGc                                             >  4:43324/1‑149 (MQ=255)                                                                                                          cGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCAGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCGATGGTGccgg                                          <  6:280597/149‑3 (MQ=255)                                                                                                            cATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCctgc                                        <  8:252530/149‑1 (MQ=255)                                                                                                             aTCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCCGCGGTAAAGGCAGACAAAGTTAAGCAGGATGTGACCATCGTAACGCTGACCAATCAGCTGACCGTTGGCCCCGGCACCAAAGGTgacaagct                                       >  2:338380/1‑142 (MQ=255)                                                                                                                ggTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTgg                                     <  1:265583/148‑1 (MQ=255)                                                                                                                  tGCTGGCAGGGGGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGGAAGCCGGCGGTTCAGGCATACAACCTTCAGCAGGATGTGACCATCGTTCCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGa                                  <  5:184007/149‑1 (MQ=255)                                                                                                                          gCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTg                          <  4:221264/149‑1 (MQ=255)                                                                                                                               cTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagcca                     <  2:219408/149‑1 (MQ=255)                                                                                                                               cTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagc                       >  6:276887/1‑147 (MQ=255)                                                                                                                               cTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagc                       >  2:100964/1‑147 (MQ=255)                                                                                                                                  ttCATTTCGGCCC‑TGGACATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagc                       >  3:45722/1‑144 (MQ=255)                                                                                                                                  ttCATTTCGGCCC‑TGGACATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagc                       <  4:45722/144‑1 (MQ=255)                                                                                                                                   tCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCg                 >  8:215460/1‑149 (MQ=255)                                                                                                                                     aTTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCa               >  5:323938/1‑149 (MQ=255)                                                                                                                                      tttCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATgg                               >  7:273945/1‑132 (MQ=255)                                                                                                                                      tttCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATgg                               <  8:273945/132‑1 (MQ=255)                                                                                                                                      tttCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCAc              >  5:182221/1‑149 (MQ=255)                                                                                                                                          ggCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTgg           >  2:239966/1‑148 (MQ=255)                                                                                                                                           gCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGa         >  4:303029/1‑149 (MQ=255)                                                                                                                                            ccc‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCAt                                                   <  7:189052/106‑1 (MQ=255)                                                                                                                                            ccc‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCAt                                                   >  8:189052/1‑106 (MQ=255)                                                                                                                                            ccc‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATCGGGGTGAGCCAGCCGCACTGGGAt        >  4:130456/1‑149 (MQ=255)                                                                                                                                             cc‑TGGGCCATG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGa         <  7:103402/147‑1 (MQ=255)                                                                                                                                             cc‑TGGGCCATG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGa         >  8:103402/1‑147 (MQ=255)                                                                                                                                                    cATGGCCCCTGGCCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATGaaaaa  <  1:338380/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   CAACTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATGAAAAA  >  NC_002947/5489633‑5489926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑