Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,489,782	G→T	6.0%	M88I (ATG→ATT) ‡	PP_4825 →	MarC family membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,489,782	0	G	T	6.0%	216.3 / 2.8	66	M88I (ATG→ATT) ‡	PP_4825	MarC family membrane protein
Reads supporting (aligned to +/- strand):  ref base G (33/29);  new base T (2/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

AACTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATGAAAAA  >  NC_002947/5489634‑5489926                                                                                                                                                      |                                                                                                                                                  aaCTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATg                                                                                                                                                   >  5:45951/1‑149 (MQ=255)  aCTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATgg                                                                                                                                                  <  1:104589/149‑1 (MQ=255)    tGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑cc                                                                                                                                               >  4:211747/1‑149 (MQ=255)       ccTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAg                                                                                                                                            <  1:305601/149‑1 (MQ=255)        gtGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAgg                                                                                                                                           >  5:193731/2‑149 (MQ=255)                 cgcgTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcgg                                                                                                                                  >  8:3350/1‑149 (MQ=255)                  gcgTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcggc                                                                                                                                 >  3:267239/1‑149 (MQ=255)                   cgTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcggcg                                                                                                                                <  7:311129/149‑1 (MQ=255)                    gTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcggcgg                                                                                                                               >  4:255526/1‑149 (MQ=255)                    gTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCcggcgg                                                                                                                               <  3:75772/149‑1 (MQ=255)                        gCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTa                                                                                                                             >  6:292586/1‑147 (MQ=255)                        gCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTAc                                                                                                                            <  3:255526/148‑1 (MQ=255)                         cAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAg                                                                                                                          <  5:90393/149‑1 (MQ=255)                           gCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGc                                                                                                                        <  3:211747/149‑1 (MQ=255)                                 gTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACaa                                                                                                                  >  2:265583/1‑149 (MQ=255)                                    gCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCAGTACAGGCAGACAACGt                                                                                                               >  5:280597/1‑149 (MQ=255)                                      cAGCGTGTTGCTGTCCCTGTTCGGTCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTAATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTc                                                                                                             <  6:238663/149‑1 (MQ=255)                                      cAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGtt                                                                                                              >  7:297167/1‑148 (MQ=255)                                      cAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTc                                                                                                             <  4:115410/149‑1 (MQ=255)                                          gtgtTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTcagca                                                                                                         <  8:166778/149‑1 (MQ=255)                                            gtTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTcagcag                                                                                                        >  8:211215/1‑148 (MQ=255)                                             ttGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGa                                                                                                      <  5:166976/149‑1 (MQ=255)                                               gCTGTACCTGTTCGGGCGGGCTATCTTCGTAATTTTCGCCATCACCGCCGATGCCTTCCGCATGGGTGCTGGCTGCGGGCTGTTCATTTCGGCCC‑TGGGCAGGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGAt                                                                                                     <  6:31689/148‑1 (MQ=255)                                                cTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGAtgt                                                                                                   <  1:214272/149‑1 (MQ=255)                                                   tACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGa                                                                                                 <  5:292586/148‑1 (MQ=255)                                                   tACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGAc                                                                                                <  7:31338/149‑1 (MQ=255)                                                   tACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGAc                                                                                                >  6:306512/1‑149 (MQ=255)                                                     ccTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCa                                                                                              >  3:221264/1‑149 (MQ=255)                                                      gttttggggcgggcGATTTGCTTATTTTGCGCCATCCCCCCGTTTGCCTGCCCCAGGGGGGGTGGCTGGGTG‑TGTTTATTTCCGCCCGTGGGCTGGG‑CCCTGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCAt                                                                                             <  6:45951/143‑1 (MQ=255)                                                         ttCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACa                                                                                                                           >  2:88088/1‑116 (MQ=255)                                                         ttCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACa                                                                                                                           <  1:88088/116‑1 (MQ=255)                                                         ttCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCg                                                                                           >  1:80506/1‑148 (MQ=255)                                                                  gcgATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGAc                                                                                 >  1:107817/1‑149 (MQ=255)                                                                  gcgATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTAATTTCGGCCC‑TGGGCATGG‑CCCTGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGAc                                                                                 <  8:147813/149‑1 (MQ=255)                                                                    gATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCa                                                                               >  6:172874/1‑149 (MQ=255)                                                                                 ttCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTCCCGCTGACCATTCCGCTGACCg                                                                   <  7:211215/148‑1 (MQ=255)                                                                                       aTCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCCATG‑CCCAggg                                                                                                                                          >  5:205132/1‑71 (MQ=39)                                                                                       aTCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCCATG‑CCCAggg                                                                                                                                          <  6:205132/71‑1 (MQ=39)                                                                                           ccgccgATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCgg                                                         >  7:252530/1‑148 (MQ=255)                                                                                               cgATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCa                                                    >  1:219408/1‑149 (MQ=255)                                                                                                      ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGAtgtg                                                                                                  >  7:307563/1‑96 (MQ=255)                                                                                                      ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGAtgtg                                                                                                  <  8:307563/96‑1 (MQ=255)                                                                                                      ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGc                                             >  6:130067/1‑149 (MQ=255)                                                                                                      ttCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGCCAAGCCGGCGGTACAGCCAGACAACGTACAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTAACCGTTGGCCCCGGCACCATCGGTGc                                             >  4:43324/1‑149 (MQ=255)                                                                                                         cGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCAGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCGATGGTGccgg                                          <  6:280597/149‑3 (MQ=255)                                                                                                           cATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCctgc                                        <  8:252530/149‑1 (MQ=255)                                                                                                            aTCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCCGCGGTAAAGGCAGACAAAGTTAAGCAGGATGTGACCATCGTAACGCTGACCAATCAGCTGACCGTTGGCCCCGGCACCAAAGGTgacaagct                                       >  2:338380/1‑142 (MQ=255)                                                                                                               ggTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTgg                                     <  1:265583/148‑1 (MQ=255)                                                                                                                 tGCTGGCAGGGGGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGGAAGCCGGCGGTTCAGGCATACAACCTTCAGCAGGATGTGACCATCGTTCCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGa                                  <  5:184007/149‑1 (MQ=255)                                                                                                                         gCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTg                          <  4:221264/149‑1 (MQ=255)                                                                                                                              cTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagcca                     <  2:219408/149‑1 (MQ=255)                                                                                                                              cTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagc                       >  2:100964/1‑147 (MQ=255)                                                                                                                              cTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagc                       >  6:276887/1‑147 (MQ=255)                                                                                                                                 ttCATTTCGGCCC‑TGGACATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagc                       <  4:45722/144‑1 (MQ=255)                                                                                                                                 ttCATTTCGGCCC‑TGGACATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGagc                       >  3:45722/1‑144 (MQ=255)                                                                                                                                  tCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCg                 >  8:215460/1‑149 (MQ=255)                                                                                                                                    aTTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCa               >  5:323938/1‑149 (MQ=255)                                                                                                                                     tttCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATgg                               <  8:273945/132‑1 (MQ=255)                                                                                                                                     tttCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATgg                               >  7:273945/1‑132 (MQ=255)                                                                                                                                     tttCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCAc              >  5:182221/1‑149 (MQ=255)                                                                                                                                         ggCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTgg           >  2:239966/1‑148 (MQ=255)                                                                                                                                          gCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGa         >  4:303029/1‑149 (MQ=255)                                                                                                                                           ccc‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCAt                                                   >  8:189052/1‑106 (MQ=255)                                                                                                                                           ccc‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCAt                                                   <  7:189052/106‑1 (MQ=255)                                                                                                                                           ccc‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATCGGGGTGAGCCAGCCGCACTGGGAt        >  4:130456/1‑149 (MQ=255)                                                                                                                                            cc‑TGGGCCATG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGa         >  8:103402/1‑147 (MQ=255)                                                                                                                                            cc‑TGGGCCATG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGa         <  7:103402/147‑1 (MQ=255)                                                                                                                                                   cATGGCCCCTGGCCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATGaaaaa  <  1:338380/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  AACTGGCCTGGAAGGTCGCGTTCGCAGCCTTGGTCGCCAGCGTGTTGCTGTACCTGTTCGGGCGGGCGATCTTCGGAATTTTCGGCATCACCGCCGATGCCTTCCGCATCGGTGCTGGCAGCGTGCTGTTCATTTCGGCCC‑TGGGCATGG‑CCCAGGGCAAGCCGGCGGTACAGGCAGACAACGTTCAGCAGGATGTGACCATCGTACCGCTGACCATTCCGCTGACCGTTGGCCCCGGCACCATCGGTGCCCTGCTGGTGATGGGGGTGAGCCAGCCGCACTGGGATGAAAAA  >  NC_002947/5489634‑5489926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑