Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,490,656	C→G	6.1%	V43L (GTT→CTT)	PP_5714 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,490,656	0	C	G	6.1%	209.4 / 3.6	65	V43L (GTT→CTT)	PP_5714	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/36);  new base G (2/2);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

GCCTGATCGGCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGACTCCTGAGGTAGTGG  >  NC_002947/5490511‑5490803                                                                                                                                                  |                                                                                                                                                      gcCTGATCGGCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTg                                                                                                                                                    >  5:129703/1‑149 (MQ=255)   cTGATCGGCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTCGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑Ct                                                                                                                                                 <  4:111442/149‑1 (MQ=255)      aTCGGCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTggg                                                                                                                                              <  4:189012/149‑1 (MQ=255)      aTCGGCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTgg                                                                                                                                               <  1:91222/148‑1 (MQ=255)      aTCGGCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTg                                                                                                                                                <  1:249081/147‑1 (MQ=255)       tCGGCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGc                                                                                                                                             <  3:228346/149‑1 (MQ=255)         ggCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCaa                                                                                                                                           <  2:117285/149‑1 (MQ=255)         ggCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCa                                                                                                                                            >  5:14847/1‑148 (MQ=255)                   gCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGc                                                                                                                                 <  2:49432/149‑1 (MQ=255)                    cccTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGcc                                                                                                                                <  4:305140/149‑1 (MQ=255)                    cccTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGc                                                                                                                                 >  5:153911/1‑148 (MQ=255)                         tCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACa                                                                                                                            <  7:117488/148‑1 (MQ=255)                               cccGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATgtc                                                                                                                     >  6:311866/1‑149 (MQ=255)                               cccGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATgtc                                                                                                                     >  6:180497/1‑149 (MQ=255)                                    tCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCAc                                                                                                                <  5:145492/149‑1 (MQ=255)                                      gAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACgg                                                                                                              <  6:129703/149‑1 (MQ=255)                                      gAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACg                                                                                                               <  1:2205/148‑1 (MQ=255)                                          cGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCtt                                                                                                          >  2:25819/1‑149 (MQ=255)                                             cGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTcgc                                                                                                       <  8:40789/149‑1 (MQ=255)                                                        gCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCt                                                                                            >  6:139697/1‑149 (MQ=255)                                                            gCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTgg                                                                                          <  3:22765/147‑1 (MQ=255)                                                            gCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGc                                                                                         >  6:130618/1‑148 (MQ=255)                                                                tCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGaa                                                                                    >  5:192912/1‑149 (MQ=255)                                                                   aaGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGa                                                                                 <  5:311866/149‑1 (MQ=255)                                                                     gCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑ATGGGCAAACGCATTGGGCAAAAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGa                                                                                 >  7:176779/1‑147 (MQ=255)                                                                               gCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAAt                                                                                   >  7:53289/1‑135 (MQ=255)                                                                               gCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAAt                                                                                   <  8:53289/135‑1 (MQ=255)                                                                               gCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGgctgct                                                                     >  5:126776/1‑149 (MQ=255)                                                                               gCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGgctgct                                                                     >  8:283915/1‑149 (MQ=255)                                                                                   ggTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTgg                                                                 <  6:14847/149‑1 (MQ=255)                                                                                    gTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGa                                                                <  5:291615/149‑1 (MQ=255)                                                                                           cAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGttt                                                         <  5:46411/149‑1 (MQ=255)                                                                                                gtTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCAc                                                    >  8:124005/1‑149 (MQ=255)                                                                                                     tGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGAca                                                                               <  8:86883/117‑1 (MQ=255)                                                                                                     tGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGAca                                                                               >  7:86883/1‑117 (MQ=255)                                                                                                         ccAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCAc                                          <  6:153911/149‑1 (MQ=255)                                                                                                         ccAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCAc                                          <  6:192912/149‑1 (MQ=255)                                                                                                             tCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCtt                                       >  2:65064/1‑148 (MQ=255)                                                                                                             tCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCtt                                       >  5:317852/1‑148 (MQ=255)                                                                                                               cGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCttga                                     <  6:317852/148‑1 (MQ=255)                                                                                                                ggCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTGG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACCAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCttgatt                                   >  1:56975/1‑149 (MQ=255)                                                                                                                     cAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGaa                                <  7:283915/147‑1 (MQ=255)                                                                                                                       ggCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAactca                            >  2:309412/1‑149 (MQ=255)                                                                                                                        gCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAactcac                           <  2:67920/149‑1 (MQ=255)                                                                                                                        gCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAactcac                           >  3:99686/1‑149 (MQ=255)                                                                                                                          cTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCt                                        <  5:179493/134‑1 (MQ=255)                                                                                                                          cTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCt                                        >  6:179493/1‑134 (MQ=255)                                                                                                                              gTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCAttt                     <  2:117400/149‑1 (MQ=255)                                                                                                                                 gAACTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCATGCTGCTGTTTACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTc                   <  8:176779/148‑1 (MQ=255)                                                                                                                                  aaGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGa                 <  7:246872/149‑1 (MQ=255)                                                                                                                                  aaGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGa                 <  6:126776/149‑1 (MQ=255)                                                                                                                                   aGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGCGGGCACCTTGATTGAACTCACTCATTTTCGa                 <  1:25819/149‑1 (MQ=255)                                                                                                                                     tCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGACTc              <  1:309412/149‑1 (MQ=255)                                                                                                                                       cccccAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGACTCCt            <  5:139697/149‑1 (MQ=255)                                                                                                                                        ccccAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGACTCCt            >  5:311773/1‑148 (MQ=255)                                                                                                                                         gcccagCAAGTTGCCTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCt                                        <  2:254184/115‑1 (MQ=255)                                                                                                                                         gcccagCAAGTTGCCTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCt                                        >  1:254184/6‑120 (MQ=255)                                                                                                                                         cccAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGgc                                                                         <  6:210746/87‑1 (MQ=255)                                                                                                                                         cccAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGgc                                                                         >  5:210746/1‑87 (MQ=255)                                                                                                                                         cccAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGcctcct            >  3:224802/1‑147 (MQ=255)                                                                                                                                          cccagCAAGTTGCCTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTggc‑g                                              <  3:241920/109‑1 (MQ=255)                                                                                                                                          cccagCAAGTTGCCTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTggc‑g                                              >  4:241920/5‑113 (MQ=255)                                                                                                                                          ccAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGACTCCTGAg         <  6:43972/149‑1 (MQ=255)                                                                                                                                                aaCTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGACTCCTGAGGTAGTg   <  4:99686/149‑1 (MQ=255)                                                                                                                                                 aCTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGACTCCTGAGGTAGTgg  >  2:329409/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                      GCCTGATCGGCAAGGCTGGCCCTGTCCGGCCCCGGTCGAGTCGCCGTGGGCGCGAGCTTGCCATCAAAGCCCAGAAACGCAAGGTTACGCCAGTAGTTCCTGAACCAGTCCGGCTCCAGGCCTTCGTTGAAGTCCCCCCAGGCAACTTG‑CTGGGCAAACGCATTGGCCAACAGGATGTCGTCACGGGCTTCGCGACTAACACCTGGCAAGAATGACAGCAGGCTGCTGTGGACGAGTTTGCCACTGGC‑GGCACCTTGATTGAACTCACTCATTTTCGACTCCTGAGGTAGTGG  >  NC_002947/5490511‑5490803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑