Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,491,490 G→A 8.2% intergenic (‑26/+26) PP_5715 ← / ← PP_5716 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,491,4900GA 8.2% 172.3 / 5.0 61intergenic (‑26/+26)PP_5715/PP_5716hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/28);  new base A (2/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

TTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGA  >  NC_002947/5491343‑5491638
                                                                                                                                                   |                                                                                                                                                    
ttAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGc                                                                                                                                                     <  7:101488/149‑1 (MQ=255)
  aGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGcc                                                                                                                                                    <  4:216072/148‑1 (MQ=255)
     ggTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCgtgt                                                                                                                                                <  5:149784/149‑1 (MQ=255)
         aGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGa                                                                                                                                             >  8:13813/1‑148 (MQ=255)
          ggTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                           <  5:151955/149‑1 (MQ=255)
            tAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAgg                                                                                                                                         <  2:19916/149‑1 (MQ=255)
              ggCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTGGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGc                                                                                                                                        <  5:185674/148‑1 (MQ=255)
               gCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcct                                                                                                                                      <  3:102839/149‑2 (MQ=255)
                   ccAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcctgga                                                                                                                                   <  4:261284/148‑5 (MQ=255)
                       cTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                              >  6:49248/1‑149 (MQ=255)
                       cTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                              <  5:49248/149‑1 (MQ=255)
                           aCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAg                                                                                                                                          >  4:230416/1‑133 (MQ=255)
                           aCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAg                                                                                                                                          <  3:230416/133‑1 (MQ=255)
                            atctcAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCag                                                                                                                         <  7:204823/145‑1 (MQ=255)
                              tCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCagag                                                                                                                       <  7:13813/149‑1 (MQ=255)
                                 aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCag                                                                                                                         >  8:204823/1‑144 (MQ=255)
                                 aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTc                                                                                                                    >  6:276221/1‑149 (MQ=255)
                                  aCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                           <  6:12958/125‑1 (MQ=255)
                                  aCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                           >  5:12958/1‑125 (MQ=255)
                                         ttCTGCTCGTGAAGCACTGACATTGTTGTTGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                <  7:290366/145‑1 (MQ=255)
                                         ttCTGCTCGTGAAGCACTGACATTGTTGTTGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                >  8:290366/1‑145 (MQ=255)
                                         ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                              >  8:12627/1‑131 (MQ=255)
                                         ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                <  1:115608/145‑1 (MQ=255)
                                         ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                >  2:115608/1‑145 (MQ=255)
                                                 gTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGAc                                                                                                     <  5:276221/148‑1 (MQ=255)
                                                    aaGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                                  >  6:21187/1‑148 (MQ=255)
                                                    aaGCACTGAAATTGTTGTCGATCAATACAAGGCTATTGCCGTTCAACCACAAAGAGTAATAGGCTAGCATAAATATTCACTACAAGACCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                                  >  2:198725/1‑148 (MQ=255)
                                                       cACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCcagcatatcatg                                                                                                  >  5:11276/1‑138 (MQ=255)
                                                              aTTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgt                                                                                       >  1:47974/1‑149 (MQ=255)
                                                               ttgttgTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtc                                                                                      <  6:168584/149‑1 (MQ=255)
                                                                        aTCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGttt                                                                             <  8:18831/149‑1 (MQ=255)
                                                                         tCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTg                                                                            <  6:120988/149‑1 (MQ=255)
                                                                          cAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTgg                                                                           >  8:116814/1‑149 (MQ=255)
                                                                               ccAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCt                                                                      <  2:47974/149‑1 (MQ=255)
                                                                                    cTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAAt                                                                 <  2:338496/149‑1 (MQ=255)
                                                                                      aTTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATgg                                                               >  2:307283/1‑149 (MQ=255)
                                                                                                aaCCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                     <  5:21187/149‑1 (MQ=255)
                                                                                                  ccACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGt                                                   >  3:92927/1‑149 (MQ=255)
                                                                                                        aGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATc                                             >  2:311236/1‑149 (MQ=255)
                                                                                                          tgtAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                     <  4:170841/139‑1 (MQ=255)
                                                                                                          tgtAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                     >  3:170841/1‑139 (MQ=255)
                                                                                                            tAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAATTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATcggct                                         >  8:45548/1‑148 (MQ=255)
                                                                                                              aTTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATcggcgg                                        <  1:311236/148‑1 (MQ=255)
                                                                                                              aTTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCggcggc                                       >  7:55058/1‑149 (MQ=255)
                                                                                                                    ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                                                                               >  7:98160/1‑87 (MQ=255)
                                                                                                                    ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                                                                               <  8:98160/87‑1 (MQ=255)
                                                                                                                    ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCg                                  >  7:173396/1‑148 (MQ=255)
                                                                                                                    ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCg                                  >  8:308558/1‑148 (MQ=255)
                                                                                                                        aTCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                                                                              <  7:299430/100‑1 (MQ=255)
                                                                                                                        aTCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                                                                              >  8:299430/1‑100 (MQ=255)
                                                                                                                         tCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTc                            <  7:116814/149‑1 (MQ=255)
                                                                                                                           aGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCaa                          <  2:308365/149‑1 (MQ=255)
                                                                                                                            gTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGaa                                                         >  1:222641/1‑117 (MQ=255)
                                                                                                                            gTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGaa                                                         <  2:222641/117‑1 (MQ=255)
                                                                                                                                       aGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGcacca                >  2:178127/1‑147 (MQ=255)
                                                                                                                                         gCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg            >  7:161459/1‑149 (MQ=255)
                                                                                                                                         gCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg            >  4:192106/1‑149 (MQ=255)
                                                                                                                                          cctgTCGACACGGCGTCGACGGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAATTTCAACACCGCACCACAgg            >  7:150934/4‑148 (MQ=255)
                                                                                                                                          cctgTCGACACGGCGTCGACGGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg            >  2:242033/4‑148 (MQ=255)
                                                                                                                                           ccGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCa          <  3:192106/149‑1 (MQ=255)
                                                                                                                                                  cGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTgg   <  7:308558/149‑1 (MQ=255)
                                                                                                                                                   gCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa  <  8:161459/149‑1 (MQ=255)
                                                                                                                                                   gCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa  <  4:92927/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTAGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGA  >  NC_002947/5491343‑5491638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑