Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,491,492 C→G 8.1% intergenic (‑28/+24) PP_5715 ← / ← PP_5716 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,491,4920CG 8.1% 189.5 / 5.5 61intergenic (‑28/+24)PP_5715/PP_5716hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (28/28);  new base G (2/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

AGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAG  >  NC_002947/5491345‑5491639
                                                                                                                                                   |                                                                                                                                                   
aGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGcc                                                                                                                                                     <  4:216072/148‑1 (MQ=255)
   ggTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCgtgt                                                                                                                                                 <  5:149784/149‑1 (MQ=255)
       aGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGa                                                                                                                                              >  8:13813/1‑148 (MQ=255)
        ggTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                            <  5:151955/149‑1 (MQ=255)
          tAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAgg                                                                                                                                          <  2:19916/149‑1 (MQ=255)
            ggCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTGGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGc                                                                                                                                         <  5:185674/148‑1 (MQ=255)
             gCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcct                                                                                                                                       <  3:102839/149‑2 (MQ=255)
                 ccAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCTGTCGACACGGCGTCGACGGGcctgga                                                                                                                                    <  4:261284/148‑5 (MQ=255)
                     cTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                               <  5:49248/149‑1 (MQ=255)
                     cTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                               >  6:49248/1‑149 (MQ=255)
                         aCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAg                                                                                                                                           <  3:230416/133‑1 (MQ=255)
                         aCATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAg                                                                                                                                           >  4:230416/1‑133 (MQ=255)
                          atctcAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCag                                                                                                                          <  7:204823/145‑1 (MQ=255)
                            tCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCagag                                                                                                                        <  7:13813/149‑1 (MQ=255)
                               aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCag                                                                                                                          >  8:204823/1‑144 (MQ=255)
                               aaCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTc                                                                                                                     >  6:276221/1‑149 (MQ=255)
                                aCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                            <  6:12958/125‑1 (MQ=255)
                                aCAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACa                                                                                                                                            >  5:12958/1‑125 (MQ=255)
                                       ttCTGCTCGTGAAGCACTGACATTGTTGTTGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                 >  8:290366/1‑145 (MQ=255)
                                       ttCTGCTCGTGAAGCACTGACATTGTTGTTGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                 <  7:290366/145‑1 (MQ=255)
                                       ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATg                                                                                                                               >  8:12627/1‑131 (MQ=255)
                                       ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                 <  1:115608/145‑1 (MQ=255)
                                       ttCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGt                                                                                                                 >  2:115608/1‑145 (MQ=255)
                                               gTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGAc                                                                                                      <  5:276221/148‑1 (MQ=255)
                                                  aaGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                                   >  6:21187/1‑148 (MQ=255)
                                                  aaGCACTGAAATTGTTGTCGATCAATACAAGGCTATTGCCGTTCAACCACAAAGAGTAATAGGCTAGCATAAATATTCACTACAAGACCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTg                                                                                                   >  2:198725/1‑148 (MQ=255)
                                                     cACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCcagcatatcatg                                                                                                   >  5:11276/1‑138 (MQ=255)
                                                            aTTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgt                                                                                        >  1:47974/1‑149 (MQ=255)
                                                             ttgttgTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTgtc                                                                                       <  6:168584/149‑1 (MQ=255)
                                                                      aTCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGttt                                                                              <  8:18831/149‑1 (MQ=255)
                                                                       tCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTg                                                                             <  6:120988/149‑1 (MQ=255)
                                                                        cAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTgg                                                                            >  8:116814/1‑149 (MQ=255)
                                                                             ccAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCt                                                                       <  2:47974/149‑1 (MQ=255)
                                                                                  cTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAAt                                                                  <  2:338496/149‑1 (MQ=255)
                                                                                    aTTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATgg                                                                >  2:307283/1‑149 (MQ=255)
                                                                                              aaCCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                      <  5:21187/149‑1 (MQ=255)
                                                                                                ccACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGt                                                    >  3:92927/1‑149 (MQ=255)
                                                                                                      aGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATc                                              >  2:311236/1‑149 (MQ=255)
                                                                                                        tgtAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                      <  4:170841/139‑1 (MQ=255)
                                                                                                        tgtAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACttt                                                      >  3:170841/1‑139 (MQ=255)
                                                                                                          tAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAATTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATcggct                                          >  8:45548/1‑148 (MQ=255)
                                                                                                            aTTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATcggcgg                                         <  1:311236/148‑1 (MQ=255)
                                                                                                            aTTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCggcggc                                        >  7:55058/1‑149 (MQ=255)
                                                                                                                  ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                                                                                >  7:98160/1‑87 (MQ=255)
                                                                                                                  ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAg                                                                                                <  8:98160/87‑1 (MQ=255)
                                                                                                                  ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCg                                   >  7:173396/1‑148 (MQ=255)
                                                                                                                  ttGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCg                                   >  8:308558/1‑148 (MQ=255)
                                                                                                                      aTCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                                                                               <  7:299430/100‑1 (MQ=255)
                                                                                                                      aTCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGtt                                                                               >  8:299430/1‑100 (MQ=255)
                                                                                                                       tCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTc                             <  7:116814/149‑1 (MQ=255)
                                                                                                                         aGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCaa                           <  2:308365/149‑1 (MQ=255)
                                                                                                                          gTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGaa                                                          >  1:222641/1‑117 (MQ=255)
                                                                                                                          gTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGaa                                                          <  2:222641/117‑1 (MQ=255)
                                                                                                                                     aGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGcacca                 >  2:178127/1‑147 (MQ=255)
                                                                                                                                       gCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg             >  7:161459/1‑149 (MQ=255)
                                                                                                                                       gCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg             >  4:192106/1‑149 (MQ=255)
                                                                                                                                        cctgTCGACACGGCGTCGACGGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAATTTCAACACCGCACCACAgg             >  7:150934/4‑148 (MQ=255)
                                                                                                                                        cctgTCGACACGGCGTCGACGGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAgg             >  2:242033/4‑148 (MQ=255)
                                                                                                                                         ccGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCa           <  3:192106/149‑1 (MQ=255)
                                                                                                                                                cGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTgg    <  7:308558/149‑1 (MQ=255)
                                                                                                                                                 gCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa   <  8:161459/149‑1 (MQ=255)
                                                                                                                                                 gCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGa   <  4:92927/149‑1 (MQ=255)
                                                                                                                                                  ccGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAg  <  3:288942/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGCGGTCAGGTAGGCTGCCAGCTGAACATCCAACAGTGCTTCTGCTCGTGAAGCACTGACATTGTTGTCGATCAATACCAGGCTATTGCCGTTCAACCACAAAGTGTAATTGGCTTGCATCAGTATGCCCTCCAGGCCCGTCGACGCCGTGTCGACAGGCCAACTTCATGGTCAGAGTTCCAGTGCCAGCAGGACCTGCAGGGCATTGTCGTCAAGTTTGGTTTCTACAATGGCGCGAACTTTGTCGTATCGGCGGCTATCGAAATTCAACACCGCACCACAGGCAAAATTGGAG  >  NC_002947/5491345‑5491639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑